Custom Builder enables you to create the arrays/plates or assays/tubes you need for your research. Simply provide QIAGEN with a list of identifiers for your genes or proteins (name, RefSeq ID, catalog number, genomic coordinates, etc.), decide your plate's layout (for arrays) and QIAGEN will create a product customized just for you! With Custom Builder, you can create customized products for mRNA expression, NGS targeted sequencing, ELISA protein analysis and analysis of siRNA and miRNA mimics and inhibitors. Start building your custom product today!

1. Sample to Insight
Building a Custom RT2 Profiler PCR Array in GeneGlobe
Samuel Rulli
Global Product Manager – RT2 Profiler PCR Arrays
Samuel.rulli@qiagen.com
Title, Location, Date 1

2. Sample to Insight
How to build a custom array for RNA-seq validation
Title, Location, Date 2
Gene Transcript Fold Change p-val q-val
IL12B NM_002187 Infinity 4.97E-04 1.20E-03
TNFSF14 NM_172014 129 8.99E-55 1.49E-52
CXCL2 NM_002089 110 3.26E-30 1.08E-28
IL1RN NM_173843 102 7.30E-51 6.06E-49
C3 NM_000064 82.4 1.66E-18 2.13E-17
CXCL9 NM_002416 47.1 5.51E-39 3.05E-37
FASLG NM_000639 35.1 2.33E-22 3.86E-21
CCL11 NM_002986 35 4.86E-11 2.99E-10
SELE NM_000450 26.5 1.22E-27 2.52E-26
TNFSF10 NM_003810 23.3 2.24E-29 5.31E-28
CD27 NM_001242 19.5 1.09E-17 1.21E-16
CCL5 NM_002985 18.6 2.53E-27 4.67E-26
SELP NM_003005 17.3 6.77E-18 8.02E-17
CD80 NM_005191 12.7 2.28E-17 2.36E-16
CASP8 NM_033355 11.3 2.76E-17 2.69E-16
IFNG NM_000619 10.8 8.81E-07 3.18E-06
IL1R1 NM_000877 9.9 1.64E-16 1.43E-15
CXCL10 NM_001565 9 4.80E-15 3.46E-14
BCL3 NM_005178 8.9 1.33E-19 1.84E-18
CCL22 NM_002990 8 3.69E-04 9.01E-04
CCNB2 NM_004701 7.7 2.10E-12 1.40E-11
MYD88 NM_002468 7.7 3.00E-17 2.77E-16
IL2 NM_000586 7.3 3.24E-03 6.47E-03
HMOX1 NM_002133 6.8 2.86E-15 2.16E-14
TNFRSF10B NM_147187 6.5 1.93E-11 1.23E-10
LTBR NM_002342 6.2 1.02E-13 7.09E-13
VCAM1 NM_001199834 5.6 2.66E-10 1.52E-09
CD69 NM_001781 5.3 1.88E-07 7.24E-07
TLR9 NM_017442 -5.00 4.57E-08 1.95E-07
CD83 NM_004233 -13.33 1.61E-21 2.44E-20
CCNE1 NM_001238 -14.93 5.97E-16 4.96E-15
CCNG2 NM_004354 -15.15 7.87E-31 3.27E-29
CCND2 NM_001759 -17.54 8.22E-30 2.27E-28
IFNA1 NM_024013 -128.21 2.76E-15 2.16E-14
52 samples with 48 genes
34 genes +/- 5 fold change
5 normalization genes
Controls for RT, PCR and gDNA
contamination
n=1 qPCR assays per sample
Biorad CFX96 instrument
26 total plates

3. Sample to Insight
How to Find the Custom RT2 PCR Array builder
Title, Location, Date 3
1.) www.qiagen.com/MyPCRArray (capitalization not important)
2.) Navigate from QIAGEN.com homepage

4. Sample to Insight
Genes to upload
Title, Location, Date 4
IL12B,TNFSF14,CXCL2,IL1RN,C3,CXCL9,FASLG,CCL11,SELE,TNFSF10,CD27,C
CL5,SELP,CD80,CASP8,IFNG,IL1R1,CXCL10,BCL3,CCL22,CCNB2,MYD88,IL2,H
MOX1,TNFRSF10B,LTBR,VCAM1,CD69,TLR9,CD83,CCNE1,CCNG2,CCND2,IFN
A1
AIRN,BANCR,FAS-AS1,PCAT1,TUG1,XIST

5. Sample to Insight
Title, Location, Date 5

6. Sample to Insight
Title, Location, Date 6

7. Sample to Insight
Title, Location, Date 7
Type in NCBI GeneSymbol,
Entrez GeneIDs, NCBI
Transcript Refseq IDs.
SangerIDs, Sanger Accessions
GeneGlobe Catalog Numbers

8. Sample to Insight
Title, Location, Date 8

9. Sample to Insight
Confirm gene list
Title, Location, Date 9
Confirm gene list

10. Sample to Insight
Title, Location, Date 10
Configure Product
Select instrument
Choose plate

11. Sample to Insight
Add controls and normalization genes
Title, Location, Date 11
! Select RT2 for only mRNA
applications. Select RT2 lncRNA for
lncRNA applications. Select both to
have option of mRNA or lncRNA from
same gene locus.

12. Sample to Insight
Configure Plate (add, move or delete genes)
Title, Location, Date 12

13. Sample to Insight
I added some controls and normalization genes
Title, Location, Date 13
Price is for minimal # plates
Add to cart to assign
Custom catalog
number

14. Sample to Insight
How many plates do I need? 15 (and I can order exactly 15!)
Title, Location, Date 14
Material # 330171 Material # 330181

15. Sample to Insight
My shopping cart. Order online or call customer care.
Title, Location, Date 15
To order online: press
“Checkout” and follow
ordering process (credit
card or PO)
To order using customer
care, call and mention
product 330171.
Quantity 1. CLAH20268.
12 plates
Product 330181.
Quantity 3. CLAH20268
Forward cart to procurement

16. Sample to Insight
Ordering process for 330171/330181
Title, Location, Date 16
Ordering online:
Customer or procurement can use credit card or PO. Once order is placed it
automatically enters SAP and once order has cleared SAP it enters production.
Calling customer care:
Customer must supply custom array number and total number of plates.
Custom Array numbers
Will start with CLA[SPECIES] ##### (5 unique numbers).
One gene list to one unique catalog number
Any changes to gene list generates new unique catalog number
Customer’s orders and gene lists will be saved online for future re-orders