2. The aim of 3D bioprinting is to construct, in vitro,
tissues, organs and other biological systems that
mimic their native counterparts
The materials used consist of natural and synthetic
polymers, living cells, drugs, growth factors
3D bioprinting is an interdisciplinary field,
requiring knowledge from developmental biology,
stem cell science, chemistry, computer science, and
16. Tissue engineering and regenerative
Transplantation and clinical
Drug testing and high-throughput
Cancer research 16
17. 7.1 Pluronic F127 hydrogel characterization and biofabrication in
cellularized constructs for tissue engineering applications
Emilia Gioffredi et. al. developed a method for printing cellularised
scaffolds from thermosensitive hydrogels.
Figure : A, B) FEG-SEM micrographs of a four-layered hydrogel
scaffold (needle Ø 200 μm, fiber spacing 600 μm); C, D) transversal
sections of the fabricated fibers.
18. 7.2 Tailoring mechanical properties of decellularized
extracellular matrix bioink by vitamin B2-induced photo-
Jinah Jang et. al. developed two-step process to solidified bio-
Figure : Schematic illustration of a two-step crosslinking mechanism that
applies concurrent crosslinking of vitamin-B2-induced covalent crosslinking
and thermal crosslinking
19. Bioprinting is one of the promising technology
expanding its horizon
Integration of bioprinting techniques to enhance the
printing process is a good strategy
Bio-inks are core of bio-printing
Different bio-inks with different bio-printing qualities
thus we need to choose one according to our need
There is no idle bio-ink for printing thus each bio-ink
can be functionalized for increasing its property
according to need of technique
Synchronization of bioprinting and bioinks can
definitely aid researchers to develop new applications
of it 19
20. Gudapati H, Dey M, Ozbolat I., 2016, “A comprehensive review on
droplet-based bioprinting: Past, present and future”, Volume 102,
Ji Eun Kim, Soo Hyun Kim, Youngmee Jung, 2016, “Current
status of three-dimensional printing inks for soft tissue
regeneration”, Volume 13, Pages 636-646.
Jia Min Lee and Wai Yee Yeong, 2016, “Design and Printing
Strategies in 3D Bioprinting of Cell-Hydrogels: A Review”, Adv.
Healthcare Mater., Volume 5, Pages 2556-2565.
Ilze Donderwinkel, Jan C. M. van Hest and Neil R. Cameron,
2017, “Bio-inks for 3D bioprinting: recent advances and future
prospects”, Polym. Chem., Volume 8, Pages 4451-4471.
Ozbolat IT, Yu Y., 2013, “Bioprinting toward organ fabrication:
challenges and future trends”, IEEE Trans Biomed Eng, Volume
60, Pages 691-699.