RAMBO PRESERVATION

1. RAMBOPRESERVATION

2. NOTHING HERE

3. RAMBOPRESERVATION
⚫FirstdocumentedbyBoyle(1663).
⚫Firstconceptofspermpreservationby
Lazzarospallanzini(1760).
⚫ Most significantdiscoverywastheuseof
glycerolascryoprotectant byPolgeand
smith(1949)

4. PRINCIPLE
1. FREEZING
2. STORING
3. THAWING

5. CONT
..
⚫ Theabilityofglycerol toprotectcellsfromfreezing
injurywasdiscoveredaccidentally
.
⚫ Thesubsequentdevelopmentof
RAMBOPRESERVATION techniqueshashada
huge impactinmanyfields, mostnotablyin
reproductivemedicine.
⚫

6. ⚫ Freezing injuryhasbeenshownto havetwo
components,direct damage fromtheicecrystals
andsecondarydamage causedbytheincreasein
concentrationofsolutesasprogressivelymoreiceis
formed.
⚫ Intracellularfreezingisgenerallylethalbutcanbe
avoided bysufficientlyslow cooling, and under
usualconditionssolutedamagedominates.

7. WHY
RAMBOPRESERVATION?
⚫ Availability and preservation of raw biological
materials are the major challenge in the
widespread use of assisted reproduction
technology in humans and livestock.
⚫ For example, many artificial insemination
procedures rely on availability of cryopreserved
sperm.
⚫ Similarly, cryopreserved oocytes and embryos are
being increasingly used in assisted reproduction

8. NOTHING HERE ALSO