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Endophytes Identification: morphological methods

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Titulo Ponencia: Endophytes Identification: morphological methods

Autor: Dr. Gary Strobel

Evento TF Innova:

Workshop Biotechnology "Isolation and identification of endophytic fungi from vascular plants"

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Endophytes Identification: morphological methods

  1. 1. Montana State University
  2. 2. Pestalotiopsis in the World’s Rainforests
  3. 3. Pestalotiopsis microspora an Endophytic Fungus 1. One of the most frequently observed endophytes. 2. Found in all rainforests and most plant species. 3. It has hundreds of synonyms. 4. Exists as many biotypes and makes hundreds of natural products. 5. Some biotypes are easily genetically transformed.
  4. 4. Pestalotiopsis microspora (culture and spores)
  5. 5. The Acervulus and Conidia of Pestalotiopsis microspora
  6. 6. Pestalosphaeria hansenii on Barley
  7. 7. The Perithecium and Ascopsores of Pestalosphaeria hansensii, Perfect Stage of Pestalotiopsis microspora
  8. 8. Picking the Spines of Cholla Cactus To Support the Bioassay Chemicals
  9. 9. Developing a Bioassay for Sex Inducing Chemicals found in Plants
  10. 10. Ability of Various Fractions of T cuspidata to Induce the formation of fruitng structures
  11. 11. The Induced Perithecia of Pestalosphaeria
  12. 12. Protoplasts of P. microspora Ready for Genetic Transformation
  13. 13. Selection of Hygromycin Resistant Isolates
  14. 14. Methylene chloride extracts of P. microspora isolates from around the world
  15. 15. Sepik River Area of Papua New Guinea
  16. 16. Sepik River Area of Papua New Guinea
  17. 17. Crystals of Pestacin
  18. 18. Pestacin-a Powerful Antioxidant
  19. 19. Isopestacin- an antioxidant
  20. 20. Sampling trees for Endophytic Fungi in the Highlands of Papua New Guinea
  21. 21. Pestalotiopsis jesterii, a fungal endophyte
  22. 22. Conidiospores of P. jesterii
  23. 23. Test Fungus Natural Synthetic MIC MIC P. Ultimum 25 25 R. Solani 25 50 G. Candidum 100 100 P. oryzae 25 25 S. sclerotiorum 100 100
  24. 24. Huli Wigmen of Papua New Guinea
  25. 25. Pestalotiopsis microspora as an endophytic rainforest fungus has made the “big time” as a producer of new natural products
  26. 26. The Orchid -Dendrochilum sp.
  27. 27. Solid State NMR of Ambuic Acid
  28. 28. Ambuic Acid is produced by various isolates of Pestalotiopsis microspora obtained in various rainforest locations around the world
  29. 29. Torreya taxifolia and torreyanic acid
  30. 30. Torreyanic acid Anticancer agent
  31. 31. Pestaloside