Presentation slides of Shojinmeat Project at New Harvest 2017 conference on Oct.11,12 of 2017 at New York City

2. 2
Yuki Hanyu
New Harvest 2017
11.Oct.2017, New York City
Shojinmeat Project -
Citizen Science for Cellular Agriculture

3. Citizen Science movement for cellular agriculture
#Food Security
#Food Miles
#Regulations
#Cooking
#Culture & thoughts
#History
#Food safety
#Ethical studies
#Animal welfare
#Regenerative medicine
#Tissue engineering
#Bioreactor
#Culture medium
#Commercialization
#LCA
#R&D
#Soc.&Econ.
Shojinmeat Project
3
#Global collaboration
#Space
#Art
The first startup
coming out

4. Overview - latest developments
4
Still self-funded and independent - not affiliated to university or corporation

5. 5
Sustainability
Dreams for
the future
Motivations
Pure fun

6. 6
Technological Social
Need bigger and cheaper
⇒Let’s biohack!
Need to be understood
⇒Let’s talk!
The hurdles

7. Regional Perspectives
7
Zen Buddhist
view on clean
meat?
Joint-sessions
with Kyoto Uni.

8. 8
Biohacking / DIY-bio?
Accessible, transparent, low-cost
“It’s all on the web!”
“You can do it too!” “We don’t need that high grade”

9. Where do we do experiments?
Leave-a-Nest teaching lab
Open Bio Club, FabCafe Tokyo
Homes and kitchens
Tissue and organ factory, TWIns lab

10. Sports drink culture media
90% DAKARA
80%
70%
60%
0%
(DMEM only)
50%
Days
Celldivisions
Proliferation of mouse L6 in DMEM/Dakara 10% FBS
#pH and osmolality of Dakara adjusted by NaHCO3(s) and 2M NaOH
Fluid name osmolality pH
DMEM(-, hi glu) 345 7.4
Pocari 338 3.4
Aquarius 291 3.37
Amino-Value 4000 289 3.63
AminoVital Gold 186 3.33
Vitamin Water 302 3.3
Green DAKARA 322 3.28
Amiiru Water 249 3.4
Mamoru Chikara 546 3.58
10

11. Yeast extract as serum alternative?
11
Commercially available:
“Yeast extract for animal cell culture”
(Sheffield Bioscience Ltd.)
Dried yeast
(dog food)
Phosphate buffer & salt
Papain, 65℃, stir, ~24h
to “digest” yeast
Filtered before
use in cell culture
pH7.4
No bacterial contamination
Preparation cost: 10¢/L

12. The limits
12
Species Cell identity sYE conc. Result
Human HeLa Human cervical cancer 10％ Successful (but for how long?)
Human Human Embryonic Kidney 293T 10％ Successful (same as above)
Mouse Germ cell 10％ Unsuccessful
Mouse Ovarian somatic cell, primary 10％ Successful (but for how long?)
Mouse Hepatocyte, primary 10％ Successful (same as above)
Mouse Fetal cell, primary 10％ Successful (same as above)
Mouse Myoblast, primary 10％ Initially successful, but cell
division halts after ~3gen.
Mouse Embryonic stem cells 10％ Unsuccessful
Yeast extracts do have limits.
Some growth factors are missing.

13. 13
Networked cell culture setup “Culnet System” supplies the
ingredients missing from yeast extract
Ohlsson C et al., 2009, Endocr Rev
Francis GL, 2010, Cytotechnology
HGF
EGF
Albumin
Transferrin
Adrenalin
“Culnet System” prototype
PCT/JP2016/067599
Japan pat. 6111510
Breaking the limits

14. 14
Clean Foie Gras
Grow
cells
Fat-load
cells
Assemble
cells

15. $50,000?
“Conventional”
$600
$6
$4000
$200
DMEM
FBS
Growth
factors
sYE
“Difficult cells”
“Easy cells”
So, where are we for 100g of meat?
Co-culture Use yeast
extract
Use food grade
DMEM/‘Gatorade’
Use food grade
yeast extract
Commercial
Culnet System
$0.6

16. Go-to-Market Roadmap
16
$300,000/kg
Foie Gras
$40/kg
“Designer
Meat”
$20/kg
i.e.
“fish fat beef”
“beef/pork cellular mix”
“DHA/Omega3 plus meat”
Meat.
$6/kg
Supplements
$3000/kg
Integriculture takes care
of those business things
Expected break-
even cost

17. 17
Designer meat prototype
“Superfood” algae-loaded designer meat
“Green
meat”

18. 18
“Steak” research
“Tissue and organ factory”
Joint
project
@
TWIns
Lab

19. Demonstration status
19
$300,000/kg
Foie Gras “Designer
Meat”
i.e.
“fish fat beef”
“beef/pork cellular mix”
“DHA/Omega3 plus meat”
Meat.
Supplements
Gaining traction
Prototype demonstrated
In progress at TWIns lab
Seed-round
fundraising

20. Seafood brewery
20

21. Meat brewery
21

22. Farm high-rise
22

23. Mars Colony
23

24. Orbital Zero-G Farm
24

25. Shojinmeat Project and Integriculture Inc.
25
How to make ⇒ Open
How to scale ⇒ Proprietary
※We need legal assistance for opensourcing
“how to make”- will Creative Commons work?
Numberindemand
Level of personalization

26. “Democratized Cellular Agriculture”
26
Academia hints
the way
Citizens act and
set the direction
Businesses scale
and deliver

27. A direction being set - “teens can do it”
27
“Growing meat at home”,
televised nationwide
2017.08.21

28. Takeaways and acknowledgements
28
Clean meat needs to be cheap
and understood.
“DIY bio” based research is
finding the way. (“Culnet System”)
Integriculture Inc. aims to
scale this technology.
Citizens set the direction and
businesses scale it.
And Everyone in Shojinmeat Project!!