You performed a wheat germ acid phosphatase enzyme assay at .pdf
You performed a wheat germ acid phosphatase enzyme assay at 37C for 15 minutes using Citrate buffer, pH4.8. The data obtained is given in the Table 2 below. 1. Complete the Table 2 . 2. Plot a double reciprocal plot (Lineweaver Burke) and a Michaelis-Menten graph. Remember to label axis and include a title and appropriate units. 3. Calculate apparent Km ( y axis) and Vmax using both plots. Show Km and Vmax values on each of the graph. 4. Attach both Michaelis-Menten and Lineweaver-Burke plot along with Standard curve to the assignment. 5. Explain how are the Km and Vmax values calculated from Michaelis Menten plot different from Lineweaver-Burke plot? Which of the two is more accurate in your opinion and why? 6. Show work for full points. Table 1: pNP Std. Curve Table 2: Wheat germ acid phosphatase assay.
You performed a wheat germ acid phosphatase enzyme assay at .pdf
You performed a wheat germ acid phosphatase enzyme assay at 37C for 15 minutes using Citrate buffer, pH4.8. The data obtained is given in the Table 2 below. 1. Complete the Table 2 . 2. Plot a double reciprocal plot (Lineweaver Burke) and a Michaelis-Menten graph. Remember to label axis and include a title and appropriate units. 3. Calculate apparent Km ( y axis) and Vmax using both plots. Show Km and Vmax values on each of the graph. 4. Attach both Michaelis-Menten and Lineweaver-Burke plot along with Standard curve to the assignment. 5. Explain how are the Km and Vmax values calculated from Michaelis Menten plot different from Lineweaver-Burke plot? Which of the two is more accurate in your opinion and why? 6. Show work for full points. Table 1: pNP Std. Curve Table 2: Wheat germ acid phosphatase assay.
Más contenido relacionado
Más de adnankhan605720
Más de adnankhan605720(20)
Último
Último(20)
You performed a wheat germ acid phosphatase enzyme assay at .pdf
- 1. You performed a wheat germ acid phosphatase enzyme assay at 37C for 15 minutes using Citrate buffer, pH4.8. The data obtained is given in the Table 2 below. 1. Complete the Table 2 . 2. Plot a double reciprocal plot (Lineweaver Burke) and a Michaelis-Menten graph. Remember to label axis and include a title and appropriate units. 3. Calculate apparent Km ( y axis) and Vmax using both plots. Show Km and Vmax values on each of the graph. 4. Attach both Michaelis-Menten and Lineweaver-Burke plot along with Standard curve to the assignment. 5. Explain how are the Km and Vmax values calculated from Michaelis Menten plot different from Lineweaver-Burke plot? Which of the two is more accurate in your opinion and why? 6. Show work for full points. Table 1: pNP Std. Curve Table 2: Wheat germ acid phosphatase assay