Advantages of biostimulants of National Academy of ciences of Ukraine and the Ministry of Education and Science of Ukraine.

1. Biostimulants application on
cereals, vegetables
and other crops
Dr. Serhiy Ponomarenko
Interdepartmental Science & Technology Center
“AGROBIOTECH” NAS and MES of Ukraine, Kyiv
February, 2015

2. 2
Interdepartmental Science & Technology Center "Agrobiotech“ NAS
and MES of Ukraine was founded in 2000 for the purpose of
strengthening of state regulation in the field of development of
ecologically safe plant growth regulators, acceleration their
introduction in agriculture of Ukraine and export abroad.

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BIOSTIMULANTS OF NATURAL ORIGIN
BIOLAN, EMISTIM C, REGOPLANT:
WIDE SPECTRUM OF USING ON 25 AGRICULTURAL CROPS
Biotechnology of micromyces cultivation from root system of ginseng
5 patents
Ginseng root
Micromyces
BRIEF TECHNOLOGY DESCRIPTION
Scientific plant

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Streptomyces
avermitiles
“Agrobiotech” produces new biostimulants
with bioprotective effects

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16.11.2012
First step in plant biotechnologyFirst step in plant biotechnology

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Our company “Agrobiotech” is able to produceOur company “Agrobiotech” is able to produce
biostimulants in large volumesbiostimulants in large volumes.. For each month we canFor each month we can
help for using our technologies up to 1 000 000 hectars.help for using our technologies up to 1 000 000 hectars.

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NE ISTC Agrobiotech has license of the Ministry of
Industrial Policy of Ukraine for manufacture of 15
biostimulants (certified as PGRs).
Products are protected by 32 patents of Ukraine, 4
patents of the Russian Federation.
They are certified for use in:
 Ukraine (15)
 Germany (1)
 Belarus (4+2 in 2014)
 Kazakhstan (4)
 China (1+1 in 2015)
 Russia (2)
 Canada (Biolan in 2014)
Certification and ProtectionCertification and Protection

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Advantages: on mining of Chinese farmers our
PGRs are working as “Tears of Angels” – in such
small quantities and with high efficacy.
 PGRs are characterized by high efficiency, low doses
of using (20-30 ml per 1 ton of seed or 1 hectare of
crops) and ecological safety (1 m2
gets 1*10-9
g of
active substance). It is nanobio-ecotechnology of XXI
centure.
 The technologies of their
application coincide with
existing agromethods.

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Organic certification issued by the “Bureau of
international certification SIC 01-10. Regulations
of organic production”

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ACHIEVEMENTS OF NOBEL LAUREATES AREACHIEVEMENTS OF NOBEL LAUREATES ARE
IMPLEMENTED IN UKRAINE (2013-2014)IMPLEMENTED IN UKRAINE (2013-2014)
The Nobel Prize in Physiology or Medicine 2006 was awarded jointly to
Andrew Z. Fire and Craig C. Mello "for their discovery of RNA interference -
gene silencing by double-stranded RNA" .
Andrew Fire Craig Mello

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ACHIEVEMENTS OF NOBEL LAUREATES AREACHIEVEMENTS OF NOBEL LAUREATES ARE
IMPLEMENTED IN UKRAINE (2013-2014)IMPLEMENTED IN UKRAINE (2013-2014)

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The mechanism of the physiological
action at the cellular level
1.1. Over the past 15 years, muchOver the past 15 years, much
attention is paid to the isolation ofattention is paid to the isolation of
eukaryotic from cells andeukaryotic from cells and
determination of the biological role ofdetermination of the biological role of
small regulatory RNAs (smallsmall regulatory RNAs (small
regulatory RNA) in RNAi (RNAregulatory RNA) in RNAi (RNA
interference), which is calledinterference), which is called
posttranscriptional gene silencingposttranscriptional gene silencing
(PTGS) in plants, animals and fungi(PTGS) in plants, animals and fungi..

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2. Gene silencing is a process which
results in either degradation or blocking
of the translation of mRNA target
molecules, it is of great importance in
the adaptive virus resistance in
protecting of the genome against
transpositions of mobile DNA -
elements and ontogenetic regulation of
gene expression.
2 types of small regulatory RNA –
miRNA (microRNA) and siRNA (short
interfering RNA) have a dominant role
in silencing.
The mechanism of the physiological
action at the cellular level

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The mechanism of the physiological
action at the cellular level
3. Si/miRNA are similar in structure
and function (characterized by
antisense structure complementary to
mRNA) and play a dual role in plants:
1) jointly with site - specific
multisubunit endo- and exonucleases,
which are components of RISC
complex (RNA - induced silencing
complex), si/mi RNA determined the
life time of each mRNA molecules.
They primarily destroy by degradation
(cleavage) or blocking (silencing) of
translation the aberrant and imperfect
on the structure molecules mRNA,
which may appear incorrectly in cells,
2) serve protective (antipatogenic and
antiparasitic) function.

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The mechanism of the physiological
action at the cellular level
4. In both cases, these
biological effects are achieved
by binding si/mi RNA with
complementary polynucleotide
link of mRNA of own cells, or
pathogenic viruses, or harmful
organisms such as nematodes.

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The mechanism of the physiological
action at the cellular level
5. Scientists propose two
approaches to increase the number
of si/mi RNA in response to
pathogenesis:
1) by introducing into cells of
additional number of gene copies of
si/mi RNA by genetic transformation,
and
2) activation of expression of own
cellular genes of si/mi RNA synthesis
by some specific inducers.

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MicroRNAs as regulators
in plant metal toxicity response
Ana B. Mendoza-Soto,
Federico Sánchez and Georgina Hernández
Schematic representation of the mode of
action of miR319, miR390, miR393, and
miR398 in response to metaltoxicity.
(A) Regulation in leaves.
(B) Regulation in roots. miRNAs are
in rectangles while their targets are in
diamonds. Arrows indicate up-regulation
and blunted lines indicated own
regulation.

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The mechanism of the physiological
action at the cellular level
The ecological and economic effectiveness of joint
use of plant growth regulators with modern
herbicides was shown for the reduction of
pesticides rates.
6. During 12 years the
Uman State Agrarian
University led by prof.
Hrytsayenko Z.M. has
carried out research
"Development of eco-
friendly production
technologies with a
minimal pesticide load
in crop rotations."

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Model of CO2 transfer from air into chloroplast
and into carbohydrates

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Biolan on winter wheat (Uman, 2011)

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 Bayer CropScience, Germany
(Grodil Maxi OD, Puma Super)
 DuPont International Operations Sarl, Switzerland
(Harmony 75, Basis 75, Calibre 75, Granstar 75)
 Nufarm GmbH & Co KG , Austria
(Dicopur F600)
 Dow AgroSciences GmbH , Austria
(Prima, Esteron 600, Lontrel 300, Lancelot 450 WG)
 Syngenta International AG, Switzerland
(Lintur 70WG, Dual Gold 960, Fusilade Forte 150)
Herbicides of 14 leading world companies (apart and
jointly with bioregulators) were used in 12-years
research work of Biology Department of the Uman
State Agrarian Institute of Horticulture:

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Effect of different rates of the herbicide Caliber and
Biolan on the weed infestation of winter wheat
crops, 2012

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Biological features of herbicide Prima (Dow
AgroSciences GmbH , Austria) application
separately and jointly with Biolan, impact on the
productivity of winter triticale (2005-2007,
Forest-steppe, Ukraine.

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2175
2982
2829
2436
2576
2345
3051
2914
2901
2000
2200
2400
2600
2800
3000
3200
1 2 3 4 5 6 7 8 9
Bacteria, HIP05 = 61-70
Explanatory notes
1. No treatment
2. No treatment + hand
weedings
3. Emistim C 10 ml/ha
4. Lintur 70 WG 120 g/ha
(67% of the norm)
5. Lintur 70 WG 150 g/ha
(83 % of the norm)
6. Lintur 70 WG 180 g/ha (the
norm)
7. Lintur 70 WG 120 g/ha +
Emistim C 10 ml
8. Lintur 70 WG 150 g/ha +
Emistim C 10 ml
9. Lintur 70 WG 180 g/ha +
Emistim C 10 ml
The results of experiments during 3 years.
The complex of soil bacteria population was increased by 40%
The total quantity of bacteria in the rhizosphere
of spring wheat plants after 25 days after
herbicide Lintur 70 WG (Syngenta) and
bioregulator Emistim C application. Uman
National University of Horticulture

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The total quantity of Micromycetes in spring
wheat plant rhizosphere after 25 days after
herbicide Lintur 70 WG and bioregulator
Emistim C application. Uman National
University of Horticulture
255
326
304
269
287
266
336
311
297
250
270
290
310
330
350
1 2 3 4 5 6 7 8 9
Micromycetes, HIP05 = 12-24
The results of experiments during 3 years.
The complex of micromecete complex was increased by 32%
Explanatory notes
1. No treatment
2. No treatment + hand
weedings
3. Emistim C 10 ml/ha
4. Lintur 70 WG 120 g/ha
(67% of the norm)
5. Lintur 70 WG 150 g/ha
(83 % of the norm)
6. Lintur 70 WG 180 g/ha (the
norm)
7. Lintur 70 WG 120 g/ha +
Emistim C 10 ml
8. Lintur 70 WG 150 g/ha +
Emistim C 10 ml
9. Lintur 70 WG 180 g/ha +
Emistim C 10 ml

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Effect of biostimulants on germination, yield
and the rate of plant infestation.
Winter wheat Oksana on the infected background,
Institute of Breeding (Odesa), 2010-2013

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Biostimulants` efficiency in comparison with traditional
chemical products – seed protectants against wheat
nematode, ground beetle, turnip moth, Oscinella (2011)
1 – amount of infected caryopsides;
2 – biological efficiency %;
*- Yunta Quadro – fungicide “Bayer Crop Science”
** - Selest Top – insecticide “Syngenta”
*** - Alpha-cypermethrin – insecticide “Bayer Crop
Science”

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Efficiency of new biostimulants in comparison
with other products – seed protectants against
agents of mold and blight of soybean (2011)
1 – amount of infected caryopsides;
2 – biological efficiency %;
* – Lamardor – fungicide “Bayer Crop Science”;
** – Yunta quattro – fungicide “Bayer Crop Science”;
*** – fungi Mucor spp., Rhizopus spp., Aspergillus spp., Penicilium spp., Tricholhecium
roseum.

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Efficiency of biostimulants in comparison with
other products – seed protectants against agents
of mold and blight of corn (2011)
1 – amount of infected caryopsides;
2 – biological efficiency %;
* – Lamardor – fungicide “Bayer Crop Science”;
** – Yunta quattro – fungicide “Bayer Crop Science”;
*** – fungi Mucor spp., Rhizopus spp., Aspergillus spp., Penicilium spp., Tricholhecium
roseum.

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It is proved that these growth inducers have unique complex
of properties: promote the harvest and its quality,
release genetic potential of variety.
Moreover, grain got in variants with application
of growth inducers , does not contain mycotoxins
and residual quantities of pesticides and
pretend to ecologically safe product.
Results for Yield of Back-to-back
testing (Odessa, 2011)

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Influence of PGRs in compositions with bacterial
products on winter wheat productivity. Chernihiv
Institute APP
37,0
40,0
38,6
37,8
43,6
44,5
30,0
32,0
34,0
36,0
38,0
40,0
42,0
44,0
46,0
1 2 3 4 5 6
Yield
Variant, % to Control:
1. Control
2. Biolan, 25 ml/t + 8,1%
3. Diasophyt, 400 ml/t + 4,3%
4. Polymixobacterin +2,1%
5. Biolan + diasophyt + 17,8%
6. Biolan + polymixobacterin + 20,2%
Bioregulators of the ISTC "Agrobiotech" enhance the development of microflora as
against a pesticide action. This is shown when used jointly Biolan with
Polymixobacterin and Diasophyt
LSD 1,5 c/ha

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Biostimulant Biolan is new
biological fertilizer?!
The diagram of
results of Biolan’s
use, its efficiency at
various levels of a
mineral food in
winter wheat
cultivation
(2008-2013)

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Soil Improvement under Biolan action
ControlOGIs
Germany

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Nematode resistance on sugar beet
with Regoplant
With biostimulants Without biostimulants
Control group seeds germinated but perished on the 5th
day,
while group with biostimulants dynamically developed.

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Biostimulants` Test for Sugar beet:
Yield and Nematicidic Efficiency*
*Tests in the Institute of Sugar Beet, Ukraine, 2010
**Number of eggs and larvae per 100 cm3
of soil

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Regoplant's influence on vegetable productivity and quality
Place of the tests: Scientific-research farm “Velykosnitynske”, Kyiv region. 2012
1. Increase of leaf surface by 15-47 cm2
/plant.
2. Increased plant resistance to diseases and pests
(aphids, Colorado beetle, powdery mildew, cercosporosis, Phytophtora infestans, Alternaria blight.
P-490

37. 37
Note: * - dry matter (soluble).
** - Total sugars, g/dm3
1. Increase of leaf surface by 15-47 cm2
/plant.
2. Increased plant resistance to diseases and pests
(aphids, Colorado beetle, powdery mildew, cercosporosis, Phytophtora infestans, Alternaria blight.
Stimpo's influence on vegetable productivity and quality
Place of the tests: Scientific-research farm “Velykosnitynske”, Kyiv region. 2012
P-490

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Regoplant influence on the productivity and
quality of vegetables
23
29
18
21,2
29
35,2
20
25,3
27,3
34,4
0
10
20
30
cucumber tomato red beet carrot white
cabbage
Yield, t/ha, Control Regoplant 50 ml/ha
Increase, %
Cucumber +26,6%
Tomato +17,7%
Red beet +21,3%
Carrot +26,5%
Cabbage +26,0%
* the quantity of diseases and pests reduced by 54%
** reduced nitrates content
*** increased content of sugars and vitamins

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Stimpo's and Regoplant's influence
on strawberry productivity and quality. 2012
P-490

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Influence of Regoplant on yield and quality of
grapes. NSC VE Tairova, 2013-2014

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Potato (Sluch and Kimeria) yield after use of
Cruiser 350 g/l (Syngenta, Switzerland) and
reduced by 20 and 40% doses with biostimulants
Biolan, Stimpo and Regoplant. 2013-2014

42. 42
Cost-effectiveness of
bioregulators on potato
Treasury crops in Euro
This technology allowed a 60% reduction in the quantity of
wireworms in the soil. Institute of Potato, 2012-2014.

43. 43
Biolan Control
Biolan on sunflowers in Drabiv fields,
Ukraine

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Biostimulants during 10 years
in Germany

45. 45
Example of Effectiveness

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The 1st
World Congress on the use of
Biostimulants in Agriculture. 2012. Strasbourg,
France

47. 47
The 1st World Congress on the use of Biostimulants in
Agriculture. 26-29 November, 2012. Strasbourg, France
More than 650 delegates representing 450 companies and organizations
from 55 countries have took part in the Congress.
The 2nd World Congress on te use of Biostimulants in Agriculture
will be in Florence, Italy, in 16-19 Nov., 2015 www.biostimulants2015.com

48. 48
“We are confident in the imminence of the time when growth regulators
will be studied to the degree where they can be ranked as the gold
reserve of the agrochemical methods invariably increasing a harvest
yield, and can be recommended for universal application”
L. A. Khristeva and coauthors, 1966
“Мы уверены в неизбежности того времени, когда регуляторы роста будут
изучены на таком уровне, что они будут названы золотым запасом агрохимических
методов, неизменно увеличивая урожайность, и могут быть рекомендованы для
универсального применения”.
Л. А. Христева и соавторы, 1966
Lastly

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CONTACT INFORMATION
Dr. Serhiy Ponomarenko
Phone: +(380-44) 559-66-17
E-mail: sponom@ukr.net
www.agrobiotech.com.ua
Interdepartmental Science & Technology Center
“AGROBIOTECH” NAS and MES of Ukraine, Kyiv