1.
T Cell Activation assay
https://www.creative-biolabs.com/car-t/

2.
As an excellent service leader in the field of antibody development and
immunotherapy, Creative Biolabs now offers one-stop CAR-T therapy
development service which has become a promising approach to antitumor
therapy. Antigen specific T cell activation assay is one of the technologies
we can provide during the one-stop service which can activate CAR-T cells
for further downstream research or clinical trials.

3.
Background
T cell activation requires at least two signals to be fully activated. The first
signal is provided by the interaction of T cell antigen-specific receptor and
the antigen-major histocompatibility complex (MHC). The second signal is
provided by co-stimulatory receptors like CD28. In engineered CAR-T cell,
CAR consists of specific scFv and signaling endodomains of CD3ζ, CD28,
and 4-1BB. Creative Biolabs offers one-stop CAR-T service from basic
research to clinical trials and beyond, and antigen specific T cell activation
assay has been a vital technology in evaluating the activation activities of
different CAR-T cell products at preclinical stage.

4.
Assay of Antigen Specific T Cell Activation
CAR-modified T cells can recognize tumor cells via binding of the CAR to
its tumor-associated antigen (TAA) independent of TCR-MHC/peptide
interactions, and the engineered signaling endodomains can help T cells be
activated and persistent. As a result, T cells are activated and thus they can
efficiently eliminate tumor cells by the secretion of perforin and granzymes
as well as the expression of FasL and tumor necrosis factor-related
apoptosis inducing ligand (TRAIL). In addition, other tumor-infiltrating
immune cells can be activated by the secretion of various cytokines.

5.
Creative Biolabs offers this assay to detect the following downstream
activities to reflect the tumor antigen specific T cell activation.
➢ T Cell Expansion
➢ Upregulation of T Cell Surface Activation Markers
➢ Differentiation Into Effector Cells
➢ Cytokine Production or Cytotoxicity
➢ Induction of Apoptosis

6.
Figure 1 Antitumor effects mediated by activated CAR-T cells.
(Cartellieri, et.al. 2010)

7.
Highlight Features
➢ Senior Ph.D. scientists and extensive experience
➢ One-stop service
➢ Superior quality
➢ Timely troubleshooting
➢ Customized assay design

8.
With years of extensive experience in the field of immunotherapy, Creative
Biolabs brings our clients a suite of relevant immune-oncology assays for a
wide range of projects. We aim to work closely with our clients to tailor
each assay to facilitate the projects and help answer their interesting
scientific questions. Creative Biolabs offers not only highly advanced
assays but also customized assays. Please don’t hesitate to let us know your
requirement, and we will be pleased to serve you wholeheartedly.

9.
About Creative Biolabs
 With the exponentially increased demand in modern novel medicine,
the CAR and TCR immune cell therapy has been one of the most
promising approaches for target-specific cancer treatment. With
integrated global resources in all aspects, Creative Biolabs provides
one-stop full CAR T/NK Cell therapy development service, including
early non-clinical research, process development, pre-clinical animal
model testing, cGMP manufacturing, and phase I-III clinical trials.

10.
Contact
 45-1 Ramsey Road, Shirley, NY 11967, USA
 Email: marketing@creative-biolabs.com
 www.creative-biolabs.com/car-t

11.
Thank You