Race-typing in North American Rust Laboratories & Their Roles in the Global Cereal Rust Monitoring System
1. Race-typing in North American Rust Laboratories
& Their Roles in the Global Cereal Rust Monitoring System
Yue Jin
Cereal Disease Laboratory (Cereal Rust Lab)
US Department of Agriculture-Agricultural Research Service
St. Paul, Minnesota, USA
Tom Fetch, Jr.
Cereal Research Centre (The Dominion Rust Research Lab)
Agriculture and Agri-Food Canada
Winnipeg, Manitoba, Canada
2. Functions of the NA Rust Labs
Pathogen biology, genetics, disease epidemiology:
• Puccinia graminis
• Puccinia triticina
• Puccinia coronata
• Puccinia hordei
• Fusarium graminearum
Host resistance:
• Sources and genetics of rust resistance
• Developing new germplasm
• Rust resistance breeding
Training:
• In collaborations with universities
3. Functions of the NA Rust Labs
Systematic surveys of cereal rusts since 1920s
• New virulences & implications to crop production
• Race dynamics & causes
• Rust epidemiology: inoculum, movement, environments
• Genetic resources: population genetics, genomics
isolates for germplasm screening
9. Rust Pathogen Deposits at CDL
30,800 accessions in liquid nitrogen storage, representing:
19 different genera
69 different species
53 different countries
Collections dating back to 1956
~1,000 accessions in temporary storage
10. Facilities & Operating Seasons
Laboratory Race-typing season for foreign rust collections
ARS CDL: December to February
ARS MD: Year-round, receiving and reviving samples
UMN-BLS3 November to March-mostly Ug99 screening
Morden Lab October to April
Ottawa Lab: October to April (with year-round potential)
18. Future Work at CDL
• Monitoring/typing Pgt races of international nurseries
-- Njoro, Debre Zeit
• Confirming critical Pgt samples/isolates
-- national surveys, special studies, etc
• Working with samples/isolates associated with alternate hosts
-- in conjunction with barberry surveys
• Continuing the analysis of global Pgt collections using molecular
diagnostic assay
• Identifying rusts on barberry
-- Eastern Africa, South Asia, Central and South America
• Developing/distributing genetic stocks
-- stem and leaf rust differentials (ARS Genebank, Aberdeen)
-- ID series, UR series (in progress)
-- stocks of new resistance genes (everyone’s responsibility)
19. Future work at AAFC
• Conduct new international stem rust virulence survey as
last one was in early 70’s (Luig)
• Interested collaborators to contact Dr. Fetch
• Countries with capacity do the rust race typing
• Countries without capacity send isolates to Dr. Fetch for
race typing
• Results to be presented in booklet form and put onto
RUSTSPORE webpage
• Representative rust isolates also will be typed on
molecular basis and compared to phenotype (Szabo, Park,
Hambleton labs)
20. Set Line Sr gene
1 ISr5Ra 5
T.m.deri. 21
Vernstein 9e
ISr7bRa 7b
2 ISr11Ra 11
ISr6Ra 6
ISr8Ra 8a
CnsSr9g 9g
3 W2691SrTt-1 36
W2691Sr9b 9b
BtSr30Wst 30
Comb. VII 17+13
4 ISr9aRa 9a
ISr9dRa 9d
W2691Sr10 10
CnsSrTmp Tmp
Ug99 Reaction on NA Differentials
22. Ug99 Reaction on Differentials
Set Line Sr gene 04KEN156
1 ISr5Ra 5 4
T.m.deri. 21 3+ T
Vernstein 9e 4
ISr7bRa 7b 3
2 ISr11Ra 11 4
ISr6Ra 6 4 T
ISr8Ra 8a 4
CnsSr9g 9g 4
3 W2691SrTt-1 36 0
W2691Sr9b 9b 4 K
BtSr30Wst 30 4
Comb. VII 17+13 2++
4 ISr9aRa 9a 4
ISr9dRa 9d 4 S
W2691Sr10 10 4
CnsSrTmp Tmp 2+
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