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Epi-fluorescent Illumination Alignment Tool / Objective Lens
Mercury and xenon arc lamps are now widely utilized as illumination sources for a
large number of investigations in wide field fluorescencemicroscopy. This tool
helps you aligning and focusing the arc lamp in a Mercury or Xenon Burner and
adjusting the lamp in a fluorescencemicroscope.
Bioimager offers an optional alignment accessory to facilitate the centering of the
image of the lamp arc to the rear aperture of the objective. At its upper end, this
accessory either has the standard Royal MicroscopicalSociety (RMS) thread or 25-
millimeter threads to accommodate newer nosepieces, and can be screwed into
the nosepiece in place of an objective. In order to use the accessory, itis first
placed in the nosepiece and then rotated into the light path. The lower face of the
accessory has a frosted, orange-colored glass window with an inscribed crosshair.
Light passing through the microscopedichromatic mirror strikes the built-in
reflector of the centering screen and is reflected onto the crosshairs. As thelamp
condenser knob and the centering screws on the lamp socketare manipulated,
the image can be observed and translated so that it is centered to the crosshairs.
The size of the image of the arc can be made bigger or smaller by manipulating
the collector lens focus position. After the arc is aligned, the centering accessory
can be replaced by a regular objective.
https://www.bioimager.net/index.php/fluorescence/bim500-fl-l-l.html

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Epi fluorescent illumination alignment tool objective lens

  • 1. Epi-fluorescent Illumination Alignment Tool / Objective Lens Mercury and xenon arc lamps are now widely utilized as illumination sources for a large number of investigations in wide field fluorescencemicroscopy. This tool helps you aligning and focusing the arc lamp in a Mercury or Xenon Burner and adjusting the lamp in a fluorescencemicroscope. Bioimager offers an optional alignment accessory to facilitate the centering of the image of the lamp arc to the rear aperture of the objective. At its upper end, this accessory either has the standard Royal MicroscopicalSociety (RMS) thread or 25- millimeter threads to accommodate newer nosepieces, and can be screwed into the nosepiece in place of an objective. In order to use the accessory, itis first placed in the nosepiece and then rotated into the light path. The lower face of the accessory has a frosted, orange-colored glass window with an inscribed crosshair. Light passing through the microscopedichromatic mirror strikes the built-in
  • 2. reflector of the centering screen and is reflected onto the crosshairs. As thelamp condenser knob and the centering screws on the lamp socketare manipulated, the image can be observed and translated so that it is centered to the crosshairs. The size of the image of the arc can be made bigger or smaller by manipulating the collector lens focus position. After the arc is aligned, the centering accessory can be replaced by a regular objective. https://www.bioimager.net/index.php/fluorescence/bim500-fl-l-l.html