This is a brief presentation about Hydrolyzed Collagen, which summarizes studies on Joint Health, Gastrointestinal Health and the incidence of ulcers in horses.

1. Horse Health – Hydrolyzed Collagen
What is Collagen? What is Rousselot ASF?
• Collagen is the most abundant protein
• Rousselot ASF is Hydrolyzed Collagen
in the body of mammals (25 – 30%).
(Hydrolyzed Gelatin).
• It is the main structural component of
connective tissue (skin, cartilage, • Rousselot ASF contains collagen peptides of
ligaments, tendons and bones). low molecular weight (2 – 5,000 Da).
• It provides connective tissue with its • Rousselot ASF is easily digestible and
ability to withstand great force and metabolizable.
stress without breaking down or
tearing. • Rousselot ASF has been shown to have a
unique and varied role in maintaining the
• As people and animals age, the ability health of connective tissue.
of the body to produce and maintain
collagen progressively declines shortly
after reaching adulthood.
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2. How is Hydrolyzed Collagen used?
Performance Nutrition – Connective Tissue
• Collagen Peptides Preferentially Accumulate in Cartilage
• Collagen Peptides Induce Production of Type II Collagen
• Collagen Peptides Reduce the Production of Catabolic Enzyme
• Collagen Peptides Reduce Catabolic Activity in Cartilage
• Osteoarthritis Study with Dogs
• Summary
Hydrolyzed Collagen is
used in joint, ligament,
tendon, ulcer, GI Health and
performance recovery
products.
Gastrointestinal Health
• Frequency of Ulcers in Horses
• Recreational Horse Study
• Effect on Gastric Lesions
• Reduction in Platelet Aggregation
• Chemotactic Attraction of Fibroblasts
• Summary
2

3. Hydrolyzed Collagen – Performance Nutrition
Contains Amino Acids
critical to maintaining and
repairing connective
tissue.
3

4. How does Hydrolyzed Collagen Work?
Contains Amino Acids
critical to maintaining and
Amino Acids % Predigested Collagen
repairing connective
Di- and Tri-Peptides
of Protein tissue.
(2,000 – 5,000 Dalton)
Glycine 20
Hydroxyproline 12
Proline 13
Rapidly absorbed into
bloodstream
Preferentially accumulates in
Chemotactic Effect
connective tissue for
on collagen
extended period of time.
producing cells
80
fibroblasts/20 OIF
Chemotactic activity,
60
40
20
0
Control Test
4

5. How is Hydrolyzed Collagen used?
Performance Nutrition – Connective Tissue
• Collagen Peptides Preferentially Accumulate in Cartilage
• Collagen Peptides Induce Production of Type II Collagen
• Collagen Peptides Reduce the Production of Catabolic Enzyme
• Collagen Peptides Reduce Catabolic Activity in Cartilage
• Osteoarthritis Study with Dogs
• How Tendons Work
Hydrolyzed Collagen is
used in
joint, ligament, tendon, ulcer
, GI Health and performance
recovery products.
Gastrointestinal Health
• Frequency of Ulcers in Horses
• Recreational Horse Study
• Effect on Gastric Lesions
• Reduction in Platelet Aggregation
• Chemotactic Attraction of Fibroblasts
• Summary
5

6. Hydrolyzed Collagen – Joint Health
Collagen Peptides “Preferentially Accumulate” in
Cartilage
A study by Oesser showed that more than 95% of C-
labeled collagen peptides taken orally are absorbed
and accumulated in the cartilage of mice as early as 12
hours after ingestion. The radioactivity was twice as
high in the group receiving hydrolyzed collagen vs. the
control group, demonstrating that collagen peptides
“preferentially accumulate” in cartilage.
Collagen Peptides Induce Production of Type II
Collagen (Cartilage)
Another study showed that applying collagen
peptides to the cartilage producing cells
(chondrocytes) lead to a dose-dependent
production of Type II Collagen, reinforcing collagen’s
role in the maintenance of connective tissue.
6

7. Hydrolyzed Collagen – Joint Health
40
Collagen Peptides Reduce Blood Concentrations of 35
ng/ml of MMP-3
Catabolic Enzyme 30
25
In a study by Weide (2004) where hydrolyzed collagen 20
Before
15
was fed to dogs, there were corresponding increases
10 After
in the blood concentration of glycine, proline and 5
hydroxyproline, as well as decreases in the enzyme 0
MMP-3, which is believed to be the key enzyme
involved in cartilage matrix degradation. Dogs w/ Joint Problems
Healthy Dogs
250
Collagen Peptides Reduce Catabolic Activity in Cartilage
200
µmol/dl of Keratan Sulphate
In a study by Appelt 2005 hydrolyzed collagen was
150
Fed Hydrolyzed Gelatin
fed to horses over a period of 10 weeks, with
Keratan Sulphate being periodically measured during
100 Control Group
the study. Sulphate is released during the
breakdown of proteoglycans in cartilage, and are a
50 marker of catabolic activity in cartilage.
0
Week 0 Week 5 Week 10
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8. Hydrolyzed Collagen – Osteoarthritis Study
Collagen Peptides Improve Mobility in Osteoarthritic Dogs
In 2009 Beynen carried out a double blind placebo-
controlled trial with privately owned osteoarthritic dogs
(N=30). During 8 weeks the dogs daily received either
Rousselot ASF or a placebo (Soy Protein Hydrolysate) as a
top dressing over their meal. Dog owners were asked to
score their dogs every 8 weeks on a scale of 0 to 10 for
different criteria. Dogs receiving Rousselot ASF showed
significant improvements in activity, stiffness and lamenees.
Potential Mechanisms for Supporting Joint Health
• Preferential Accumulation in Cartilage
• Collagen Peptides Stimulate Type II Collagen Growth
• Reduce Blood Concentrations of Catabolic Enzyme
• Reduce Catabolic Activity in Cartilage
8

9. How is Hydrolyzed Collagen used?
Performance Nutrition – Connective Tissue
• Collagen Peptides Preferentially Accumulate in Cartilage
• Collagen Peptides Induce Production of Type II Collagen
• Collagen Peptides Reduce the Production of Catabolic Enzyme
• Collagen Peptides Reduce Catabolic Activity in Cartilage
• Osteoarthritis Study with Dogs
• How Tendons Work
• Tendon & Ligament Injuries and Healing Process
• Summary
Hydrolyzed Collagen is
used in
joint, ligament, tendon, ulce
r, GI Health and
performance recovery
products.
Gastrointestinal Health
• Frequency of Ulcers in Horses
• Recreational Horse Study
• Effect on Gastric Lesions
• Reduction in Platelet Aggregation
• Chemotactic Attraction of Fibroblasts
• Summary
9

10. Hydrolyzed Collagen – Gastric Ulcers
Frequency of Gastric Ulcers in Horses
% of Horses with Ulcer
90
80
In 2005 Pellegrini did a large-scale necroscopic study of
70
both recreational and performance horses. The results
60
were published in the Journal of Equine Veterinary
50
Science. There were 365 horses in the recreational
40
group and 180 horses in the performance group. 55% of
30
the recreational horses and 87% of the performance
20
horses were found to have gastric ulcers.
10
0
Recreational Performance
Recreational Horse Study
7
In 2005 McClure did a study observing the the effect that
# of Horses with Ulcer
6 weekend show travel can have on a horse’s gastro-intestinal
5 health. There were 10 horses in both the control and test
4 group. Endoscopic analysis was used to determine that all
horses in the study had no gastric ulcers at the beginning of
3
the study. The control group remained on-site, while the test
2 group was transported 4 hours away by trailer on Day 1, then
1 returned back to original site on Day 4.
0
Control Test
10

11. Hydrolyzed Collagen – Gastric Ulcers
30
Effect of Hydrolyzed Collagen on Gastric Lesions 25
Damage area, mm2
20
In a study by Samonina in 2000, Hydrolyzed Collagen’s effect on gastric
15
lesions was measured. Gastric mucosal damage was induced in live rats by
10
oral administration of ethanol. For seven days the rats were fed a
supplement containing Hydrolyzed Collagen, while the control group was 5
fed their usual diet. At the end of the study, the gastric lesions of all the 0
mice were measured. Control Test
Test group given Hydrolyzed Collagen for 7 days
10
Hydrolyzed Collagen – Platelet Aggregation
8
Platelet Aggregation
6 In the same study by Samonina, platelet aggregation was measured
Index
in the blood plasma. Platelet aggregation is the clumping together of
4 platelets in the blood, and is part of the sequence leading up to the
2 formation of clots in the blood. Reduced platelet aggregation is one
of the factors which improves microcirculation, which improves
0 gastric mucosal blood flow.
Control Test
Test group given Hydrolyzed Collagen for 10 days
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12. Hydrolyzed Collagen – Gastric Ulcers
70
Fibroblast Chemotactic Response to Hydrolyzed 60
OIF
activity, fibroblasts/20
Chemotactic
Collagen 50
40
In a study by Postlethwaite in 1976, collagen producing cells
30
(fibroblasts) were shown to have a chemotactic response to
hydrolyzed collagen. Chemotaxis is when organisms direct their 20
movement according to certain chemicals in their environment. In 10
the experiment, fibroblast migration was measured within in vitro
0
chemotaxis chambers.
Control Test
Fibroblast activity measured after 150 minutes of
incubation in the chamber
Mechanisms for Supporting Gastric Mucosal Health
• Essential building blocks for maintenance and repair of connective tissue.
• Reduces blood plasma levels of Gastrin (Castro 2010). Gastrin is the peptide hormone which
stimulates acid secretion in the stomach.
• Reduces blood plasma platelet aggregation, improving microcirculation, improving
gastric mucosal blood flow.
• Induces chemotactic response on collagen producing cells (fibroblasts).
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