2008 APS meeting, Phytophthora Workshop

1. Two simple inoculation
methods for Phytophthora
blight and root rot studies
Mike Benson Steve Tjosvold
NC State University University of California
Cooperative Extension,
Raleigh
Watsonville
NC STATE Ornamental Pathology

2. Trial
Hinodegiri azalea

3. Phytophthora inoculum
• Rice grain method
– 25 g long grain rice
– 18 ml deionized water
– Autoclave 40 min
Holmes, KA and Benson, DM. 1994
Plant Disease 78:193-199.

4. Next day
• ‘Stir up’ rice mat with bent rod

5. Loosen mat separate grains
• Autoclave 2nd time: 40 min