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Ajuga bracteosa L., and Otostegia limbata L., are two plant species of family Lamiaceae. The crude methanol leaves extracts of these two medicinal plants were examined for their antibacterial, antifungal and antioxidant (radical scavenging) activity using 1,1-diphenyl-2- picrylhydrazyl (DPPH) free radical against clinically important species of bacteria and fungi were examined. Leaves extracts of these two plants were screened against six strains of bacteria (two were gram positive i.e. Bacillus subtilis and Staphylococcus aureus and four were gram negative i.e. Vibrio cholerae, Enterobacter aerogenes, Escherichia coli and Klebsiella pneumonia) and two strains of fungi (Aspergillus niger and Aspergillus fumigatus). Eight concentrations (15 mg/ml, 12.5 mg/ml, 10 mg/ml, 7.5 mg/ml, 5 mg/ml, 3 mg/ml, 2 mg/ml and 1 mg/ml) were used to check the antimicrobial activity of plant extracts. Maximum inhibitory zone 30 mm was observed in Otostegia limbata and 25 mm in Ajuga bracteosa at 15 mg/ml mm for antibacterial activity. Ajuga bracteosa and Otostegia limbata gave response against both Aspergillus niger and Aspergillus fumigatus. This study establishes the effective ethnomedicinal use of these plants in the treatment of various infectious diseases. There is high potential for the exploitation of the plants for development of novel antimicrobial agents.
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