1. Introduction to histology
C. Jayawardena, Basic Sciences,
08/09/2010

2. I want you to understand ....I want you to understand ....
 What is histology?
 Why do we learn histology?
 What is the structural organization of the body?
 What do we learn in histology?
 How are we tested for histology?
 How do we learn histology?
 Microscopes
 References

3. Histology/ Microscopic anatomyHistology/ Microscopic anatomy
 Gross anatomy
 Macroscopic anatomy
 Developmental anatomy
 Clinical anatomy
 Surgical anatomy
 Pathological anatomy

4. Why do you learn histologyWhy do you learn histology

5. The Hierarchy of Structural OrganizationThe Hierarchy of Structural Organization
C
r
e
a
t
u
r
e
Atoms
Organelles
Epithelium
Connective
tissue
Muscles
Nerves
Bones
Epithelium
Connective
tissue
Muscles
Nerves
Bones
Heart
Lungs
Liver
Stomach
Skin
Heart
Lungs
Liver
Stomach
Skin
CVS
RS
GIS
GUS
NS
IS
CVS
RS
GIS
GUS
NS
IS

6. The Hierarchy of Structural OrganizationThe Hierarchy of Structural Organization
A group of
cells
performing a
common
function
A group of
cells
performing a
common
function
A discrete
structure
made up of
more than
one tissue
A discrete
structure
made up of
more than
one tissue
Organs
working
together for
a common
purpose
Organs
working
together for
a common
purpose
Cells and
their
functional
subunits
Cells and
their
functional
subunits
MoleculesMolecules
Copyright @ Perason Education

7. Normal structure Normal functions
Healthy life

8. Abnormal structure
No functions
Diseased

9. To Objectives
To understand
 How cells and tissues are arranged in the normal
organ system of the body,
and
 How these cells and tissues are specialized to
perform the functions (structure-function
relationship) most effectively.
Why do you learn histologyWhy do you learn histology

10. What do you learn in histologyWhat do you learn in histology

11. Learn the (microscopic) structure whileLearn the (microscopic) structure while
correlating the structure with functionscorrelating the structure with functions
Physiological
Biochemical
Pathological / diseased
Treatment
Prevention

12. How are you tested for histologyHow are you tested for histology
 Theory -
o MCQ
o SAQ
 Practical –
o Spots (OSPE)

13. How are you tested for histologyHow are you tested for histology
BS 1103 – Tissues of the body
BS 1105 –Teeth and supporting
BS 1106 – Cardiovascular
BS 1107 – Respiratory
BS 1108 – Gastrointestinal
--- 11 / 18

15. 1.1 Identify precisely
1.2 Give named example for 1.1 in the body
1.3 Identify the structure indicated by the pointer
1.4 Give two reasons for your answer in 1.3

16. How do you learn histologyHow do you learn histology

17. How do you learn histologyHow do you learn histology
 Practical
 Come up with idea what you are looking for today
 e.g What do you expect to see in the stratified
squamous keratinizing epithelium
 See those features in the sections by your-self
 Find answers for the questions
 Actively involve in the discussion

18. http://www.lab.anhb.uwa.edu.au/mb140/

19. Practical
Examination
 What do you expect to see in the
particular tissue or organ (Preparation)
 See those features in the sections by
your-self (Practical)
 How do they appear
 Recall
 See features seen in the section
 From the features, make a identification

20. MicroscopesMicroscopes
 Light microscopy
 Polarized microscopy
 Confocal microscopy
 Fluorescence microscopy
 Electron microscopy
 TEM – Transmitted EM
 SEM – Scanning EM

21. Light microscopyLight microscopy
Gartner and Hiatt. Color Textbook of Histology. 1997.
Maximal resolving power:
(The smallest distance between 2
points at which they can be seen as
separate spots)
LM; 0.2µm

22. Electron microscopyElectron microscopy
Gartner and Hiatt. Color Textbook of Histology. 1997. Figure 1.1.

23. TEM LMTEM LM
Gartner and Hiatt. Color Textbook of Histology. 1997. Figure 1.1.

24. ReferencesReferences
http://www.lab.anhb.uwa.edu.au/mb140/