Paper Chromatography

What is Chromatography? Chromatography is a technique for separating mixtures into their components in order to analyze, identify, purify, and/or quantify the mixture or components. Separate ,[object Object],[object Object],[object Object],[object Object],Components Mixture

Uses for Chromatography ,[object Object],[object Object],[object Object],[object Object],[object Object]

Uses for Chromatography ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Definition of Chromatography ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Illustration of Chromatography Mixture Components Separation Stationary Phase Mobile Phase Components Affinity to Stationary Phase Affinity to Mobile Phase Blue ---------------- Insoluble in Mobile Phase Black         Red        Yellow          

Types of Chromatography ,[object Object],[object Object],[object Object],[object Object],Types of Chromatography

(A) uses charge, (B) uses pores, and (C) uses covalent bonds to create the differential affinities among the mixture components for the stationary phase.

Principles of Paper Chromatography ,[object Object],[object Object],[object Object]

Paper Chromatography Experiment What Color is that Sharpie?

Overview of the Experiment ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Materials List

Preparing the Isopropanol Solutions ,[object Object],[object Object]

Preparing the Chromatography Strips ,[object Object],[object Object],[object Object],[object Object]

Developing the Chromatograms ,[object Object],[object Object],[object Object],[object Object],[object Object]

Observing the Chromatograms Concentration of Isopropanol 0% 20% 50% 70% 100%

Black Dye ,[object Object],[object Object],[object Object],Concentration of Isopropanol 0% 20% 50% 70% 100%

Blue Dye Concentration of Isopropanol 0% 20% 50% 70% 100% 1. Dye separated – blue 2. Not very soluble in low concentrations of isopropanol 3. Completely soluble in high concentrations of isopropanol

Green Dye Concentration of Isopropanol 0% 20% 50% 70% 100% 1. Dye separated – blue and yellow 2. Blue – Soluble in concentrations of isopropanol >20% 3. Yellow – Soluble in concentrations of isopropanol >0%

Red Dye ,[object Object],[object Object],[object Object],Concentration of Isopropanol 0% 20% 50% 70% 100% 3. Red – slightly soluble in low concentrations of isopropanol, and more soluble in concentrations of isopropanol >20%

Paper Chromatography

Paper Chromatography

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Paper Chromatography