The document discusses the Lottia gigantea genome project. It summarizes that L. gigantea was selected out of six mollusk species for genome sequencing. The L. gigantea genome was sequenced by the DOE JGI. The developmental lifecycle of L. gigantea is described including fertilization through to the adult stage. While aquaculture of L. gigantea has had mixed success, studies on GABA neurons and metamorphosis are discussed.

1. Molluscan genomics and the Lottia gigantea genome project 2010 Bay Area Malacologists Meeting Eric E. Gonzales University of California at Berkeley Rokhsar Laboratory

2. Six species were assessed in selecting L.gigantea for sequencing. scabra scutum digitalis pelta limatula gigantea California coast Lottia gigantea

3. The L.gigantea genome was sequenced by the DOE JGI. Collection Libraries Sequencing Assembly Annotation Release 1 2 3 4 5 6

4. L.gigantea genome tinyurl.com/LgiGenome LgiEngrailed1

5. L.gigantea developmental lifecycle Fertilization Cleavage Organizer Trochophore Veliger Metamorphosis Adult

6. Aquaculture of L.gigantea has met with mixed success. Gaba Percent Metamorphosis Competent larva GABAnergic neurons Laboratory aquaculture

7. SEM and confocal datasets are central to establishing a developmental digital atlas. Ventral Dorsal SEM CSP

8. A 96-well in situ protocol detects new expression patterns for low-abundance genes. Ventral Posterior Lateral Lgi-Bra 15 hours

9. Organizer loss and duplication transforms the body plan. Anti-MAPK Light Body plan Control Radial Twin

10. Isolated trochoblasts undergo normal development. 88 cells Trochophore Embryo Cultured Isolation