1. Made by :
Group# C1
Fasahat Ahmed Butt (36)

2.  A culture medium is a compound , which has
constituents that are essential for life ,
multiplication and growth of micro-organisms.
1) Nutrients
2) Mineral salts
3) suitable pH
4)Accessory growth factors

4. (A) LIQUID MEDIUM:
1) Peptone water
2) Nutrient Broth
(B) SOLID MEDIUM:
1) Nutrient agar

5.  Simple media
 Enriched media
 Differential media
 Selective media
 Transport media

6.  Consists of only basic substances such as,
nitrogen , carbon , minerals.
 EXAMPLES:
 Nutrient broth
 Nutrient agar
 Peptone water

7.  Some enriched material like blood and serum is
added to the medium , required for proper
growth of some bacteria
 EXAMPLES:
 Blood agar
 Chocolate agar

8.  This type of media is used to differentiate
between two groups of bacteria .
 EXAMPLES:
 MacConkey’s medium

9.  An inhibitory substance is added to the media
which prevents growth of all organisms except
the one for which it is designed.
 EXAMPLES:
 Loweistein jensen’s
medium

10.  A media used to transport specimen to keep
the organism alive
 EXAMPLES:
 AMIE’s medium

11. HOW TO IDENTIFY
BACTERIA’S USING
CULTURE MEDIA ????

12.  MICRO-ORGANISMS CULTURED:
 Staph.aureus golden yellow colonies
 Staph.citrus lemon yellow colonies
 Pseudomonas green colonies

13.  MICRO-ORGANISMS CULTURED:
 Complete (beta) hemolysis:
 Strep.pyogenes
 In complete (alpha) hemolysis:
 Streptococcus pnuemonia
 Non-hemolytic :
staph.aureus and staph.citrus

14.  MICRO-ORGANISMS CULTURED:
1)H.influenza
2)Neisseria.meningitidis

15.  MICRO-ORGANISMS CULTURED:
 1) lactose fermenters (pink colonies):
E.coli.
 2) non-lactose fermenters (colour less colonies):
shigella

16.  MICRO-ORGANISMS CULTURED:
 Mycobacterium tuberculosis