SlideShare una empresa de Scribd logo
1 de 16
Descargar para leer sin conexión
trp OPERON
Amal george
MSc Biochemistry
TRAP
OPERON
In genetics, an operon is a functioning unit of genomic
DNA containing a cluster of genes under the control of a
single promoter
Promoter sequences - DNA sequences that define where
transcription of a gene by RNA polymerase begins
Operator - a segment of DNA to which a
transcription factor binds to regulate gene
expression by repressing it
Structural genes - genes that codes for any
RNA or protein product other than a regulatory
factor
HISTORY
Discovered in 1953 by Jacques Monod and
colleagues
The trp operon in E. coli was the first
repressible operon to be discovered
Jacques Lucien Monod
Negative regulatory operon
trp OPERON
• group of genes that transcribed, together&codes
for the components for production of tryptophan
trp OPERON - structure
trp Operon Gene Gene Function
P/O Promoter; operator sequence is found in the promoter
trp L
Leader sequence; attenuator (A) sequence is found in
the leader
trp E Gene for anthranilate synthetase subunit1
trp D Gene for anthranilate synthetase subunit2
trp C Gene for glycerolphosphate synthetase
trp B Gene for tryptophan synthetase subunit1
trp A Gene for tryptophan synthetase subunit
1
2
3
trp-repressible operon
The binding of the effector molecule to the repressor greatly
increases the affinity of repressor for the operator and the
repressor binds and stops transcription.
A regulator gene, regulator, or regulatory gene is a gene encodes repressor
protein
Corepressor
Corepressor is a substance that inhibits the expression of
genes
Repressor proteins can be DNA- or RNA-binding: DNA-binding repressors - block
the binding of RNA polymerase to the promoter
Inducible operon(lac)
repressible operon(trp)
Regulation – trp operon
The trp operon is controlled by both
* repressor protein binding to the operator region
*translation-induced transcriptional attenuation
Repression
tryptophan is present in the environment
tryptophan is absent in the environment
1
2
Attenuation
Transcriptional attenuation is a regulatory mechanism that causes
premature termination of transcription under certain conditions
21
Attenuation loops
anti-termination hairpin
termination hairpin
TRAP
tryptophan mediates transcription and
translation of the genes responsible for its
biosynthesis. Tryptophan accomplishes this
through interactions with a regulatory protein
known as tryptophan-activated RNA binding
attenuation protein (TRAP).
Thank you.
Trp -repressor binding to operator DNATrp-repressor dimer

Más contenido relacionado

La actualidad más candente (20)

DNA footprinting
DNA footprintingDNA footprinting
DNA footprinting
 
Complementary DNA (cDNA) Libraries
Complementary DNA 	(cDNA) LibrariesComplementary DNA 	(cDNA) Libraries
Complementary DNA (cDNA) Libraries
 
TRANSPOSABLE ELEMENTS
TRANSPOSABLE ELEMENTSTRANSPOSABLE ELEMENTS
TRANSPOSABLE ELEMENTS
 
RNA editing
RNA editingRNA editing
RNA editing
 
Transcription in prokaryotes
Transcription in prokaryotesTranscription in prokaryotes
Transcription in prokaryotes
 
DNA Supercoiling
DNA Supercoiling DNA Supercoiling
DNA Supercoiling
 
Promoters
PromotersPromoters
Promoters
 
Transcription in prokaryotes
Transcription in prokaryotesTranscription in prokaryotes
Transcription in prokaryotes
 
Eukaryotic DNA replication
Eukaryotic DNA replicationEukaryotic DNA replication
Eukaryotic DNA replication
 
Regulation of gene expression in eukaryotes
Regulation of gene expression in eukaryotesRegulation of gene expression in eukaryotes
Regulation of gene expression in eukaryotes
 
repetitive and non repetitive dna.pptx
repetitive and non repetitive dna.pptxrepetitive and non repetitive dna.pptx
repetitive and non repetitive dna.pptx
 
Trp operon
Trp operonTrp operon
Trp operon
 
Transposones
TransposonesTransposones
Transposones
 
operon concept
 operon concept operon concept
operon concept
 
cDNA Library
cDNA LibrarycDNA Library
cDNA Library
 
Translation in eukaryotes
Translation in eukaryotesTranslation in eukaryotes
Translation in eukaryotes
 
Transcription in eukaryotes
Transcription in eukaryotesTranscription in eukaryotes
Transcription in eukaryotes
 
Site directed mutagenesis
Site directed mutagenesisSite directed mutagenesis
Site directed mutagenesis
 
Mismatch Repair Mechanism
Mismatch Repair MechanismMismatch Repair Mechanism
Mismatch Repair Mechanism
 
Eukaryotic transcription
Eukaryotic transcriptionEukaryotic transcription
Eukaryotic transcription
 

Similar a Trp operon (20)

Trp operon
Trp operonTrp operon
Trp operon
 
Trp operon
Trp operonTrp operon
Trp operon
 
Trp operon
Trp operon Trp operon
Trp operon
 
Lac operon sohil
Lac operon sohilLac operon sohil
Lac operon sohil
 
Trp operon
Trp operonTrp operon
Trp operon
 
Trp operon
Trp operonTrp operon
Trp operon
 
Gene regulation prokaryote spptx
Gene regulation prokaryote spptxGene regulation prokaryote spptx
Gene regulation prokaryote spptx
 
Lac Operon
Lac OperonLac Operon
Lac Operon
 
Ambe 101 @ lec 9-trp operon
Ambe 101 @ lec 9-trp operonAmbe 101 @ lec 9-trp operon
Ambe 101 @ lec 9-trp operon
 
2. Tryptophan operon.ppt
2. Tryptophan operon.ppt2. Tryptophan operon.ppt
2. Tryptophan operon.ppt
 
Tryptophan
TryptophanTryptophan
Tryptophan
 
Gene regulation and operon concept2.pptx
Gene regulation and operon concept2.pptxGene regulation and operon concept2.pptx
Gene regulation and operon concept2.pptx
 
Gene regulation
Gene regulationGene regulation
Gene regulation
 
Gene expression
Gene expressionGene expression
Gene expression
 
Transcription dna2011
Transcription dna2011Transcription dna2011
Transcription dna2011
 
Tryptophan Operon GP 102.pptx
Tryptophan Operon GP 102.pptxTryptophan Operon GP 102.pptx
Tryptophan Operon GP 102.pptx
 
Trp Operon.pptx
Trp Operon.pptxTrp Operon.pptx
Trp Operon.pptx
 
Tryptophan Operon
Tryptophan OperonTryptophan Operon
Tryptophan Operon
 
biochemistry Regulation of gene expression
biochemistry Regulation of gene expressionbiochemistry Regulation of gene expression
biochemistry Regulation of gene expression
 
Gene expression and regulation
Gene expression and regulationGene expression and regulation
Gene expression and regulation
 

Más de SKYFALL

Structure of amino acids
Structure of amino acidsStructure of amino acids
Structure of amino acidsSKYFALL
 
ALL ABOUT VITAMINS VITAMIN B6, B7, B12 AND FOLIC ACID
ALL ABOUT VITAMINS VITAMIN B6, B7, B12 AND FOLIC ACIDALL ABOUT VITAMINS VITAMIN B6, B7, B12 AND FOLIC ACID
ALL ABOUT VITAMINS VITAMIN B6, B7, B12 AND FOLIC ACIDSKYFALL
 
Bacteriophages
Bacteriophages  Bacteriophages
Bacteriophages SKYFALL
 
Quality control
Quality controlQuality control
Quality controlSKYFALL
 
Nutrition in pregnancy and lactation
Nutrition in pregnancy and lactation Nutrition in pregnancy and lactation
Nutrition in pregnancy and lactation SKYFALL
 
RIA, IMMUNOFLURESCENCE ASSAY
RIA, IMMUNOFLURESCENCE ASSAYRIA, IMMUNOFLURESCENCE ASSAY
RIA, IMMUNOFLURESCENCE ASSAYSKYFALL
 
Structural aberrations of chromosomes
Structural aberrations of chromosomesStructural aberrations of chromosomes
Structural aberrations of chromosomesSKYFALL
 
The citric acid cycle
The citric acid cycleThe citric acid cycle
The citric acid cycleSKYFALL
 
Regulation of glycolysis and gluconeogenesis
Regulation of glycolysis and gluconeogenesisRegulation of glycolysis and gluconeogenesis
Regulation of glycolysis and gluconeogenesisSKYFALL
 
Rna pol ii
Rna pol iiRna pol ii
Rna pol iiSKYFALL
 
Complement fixation
Complement fixationComplement fixation
Complement fixationSKYFALL
 
Cerebrospinal fluid
Cerebrospinal fluidCerebrospinal fluid
Cerebrospinal fluidSKYFALL
 
Density gradient centrifugation
Density gradient centrifugationDensity gradient centrifugation
Density gradient centrifugationSKYFALL
 
Thyroid hormone disorders
Thyroid hormone disordersThyroid hormone disorders
Thyroid hormone disordersSKYFALL
 

Más de SKYFALL (15)

Structure of amino acids
Structure of amino acidsStructure of amino acids
Structure of amino acids
 
ALL ABOUT VITAMINS VITAMIN B6, B7, B12 AND FOLIC ACID
ALL ABOUT VITAMINS VITAMIN B6, B7, B12 AND FOLIC ACIDALL ABOUT VITAMINS VITAMIN B6, B7, B12 AND FOLIC ACID
ALL ABOUT VITAMINS VITAMIN B6, B7, B12 AND FOLIC ACID
 
Bacteriophages
Bacteriophages  Bacteriophages
Bacteriophages
 
Quality control
Quality controlQuality control
Quality control
 
Nutrition in pregnancy and lactation
Nutrition in pregnancy and lactation Nutrition in pregnancy and lactation
Nutrition in pregnancy and lactation
 
RIA, IMMUNOFLURESCENCE ASSAY
RIA, IMMUNOFLURESCENCE ASSAYRIA, IMMUNOFLURESCENCE ASSAY
RIA, IMMUNOFLURESCENCE ASSAY
 
GLP
GLPGLP
GLP
 
Structural aberrations of chromosomes
Structural aberrations of chromosomesStructural aberrations of chromosomes
Structural aberrations of chromosomes
 
The citric acid cycle
The citric acid cycleThe citric acid cycle
The citric acid cycle
 
Regulation of glycolysis and gluconeogenesis
Regulation of glycolysis and gluconeogenesisRegulation of glycolysis and gluconeogenesis
Regulation of glycolysis and gluconeogenesis
 
Rna pol ii
Rna pol iiRna pol ii
Rna pol ii
 
Complement fixation
Complement fixationComplement fixation
Complement fixation
 
Cerebrospinal fluid
Cerebrospinal fluidCerebrospinal fluid
Cerebrospinal fluid
 
Density gradient centrifugation
Density gradient centrifugationDensity gradient centrifugation
Density gradient centrifugation
 
Thyroid hormone disorders
Thyroid hormone disordersThyroid hormone disorders
Thyroid hormone disorders
 

Último

Isabelle Diacaire - From Ariadnas to Industry R&D in optics and photonics
Isabelle Diacaire - From Ariadnas to Industry R&D in optics and photonicsIsabelle Diacaire - From Ariadnas to Industry R&D in optics and photonics
Isabelle Diacaire - From Ariadnas to Industry R&D in optics and photonicsAdvanced-Concepts-Team
 
World Water Day 22 March 2024 - kiyorndlab
World Water Day 22 March 2024 - kiyorndlabWorld Water Day 22 March 2024 - kiyorndlab
World Water Day 22 March 2024 - kiyorndlabkiyorndlab
 
TORSION IN GASTROPODS- Anatomical event (Zoology)
TORSION IN GASTROPODS- Anatomical event (Zoology)TORSION IN GASTROPODS- Anatomical event (Zoology)
TORSION IN GASTROPODS- Anatomical event (Zoology)chatterjeesoumili50
 
Breast Cancer Pharmacology Presentation - Louis Pearce.pptx
Breast Cancer Pharmacology Presentation - Louis Pearce.pptxBreast Cancer Pharmacology Presentation - Louis Pearce.pptx
Breast Cancer Pharmacology Presentation - Louis Pearce.pptxLouisPearce2
 
Physics Serway Jewett 6th edition for Scientists and Engineers
Physics Serway Jewett 6th edition for Scientists and EngineersPhysics Serway Jewett 6th edition for Scientists and Engineers
Physics Serway Jewett 6th edition for Scientists and EngineersAndreaLucarelli
 
SUKDANAN DIAGNOSTIC TEST IN PHYSICAL SCIENCE ANSWER KEYY.pdf
SUKDANAN DIAGNOSTIC TEST IN PHYSICAL SCIENCE ANSWER KEYY.pdfSUKDANAN DIAGNOSTIC TEST IN PHYSICAL SCIENCE ANSWER KEYY.pdf
SUKDANAN DIAGNOSTIC TEST IN PHYSICAL SCIENCE ANSWER KEYY.pdfsantiagojoderickdoma
 
RCPE terms and cycles scenarios as of March 2024
RCPE terms and cycles scenarios as of March 2024RCPE terms and cycles scenarios as of March 2024
RCPE terms and cycles scenarios as of March 2024suelcarter1
 
SCIENCE 6 QUARTER 3 REVIEWER(FRICTION, GRAVITY, ENERGY AND SPEED).pptx
SCIENCE 6 QUARTER 3 REVIEWER(FRICTION, GRAVITY, ENERGY AND SPEED).pptxSCIENCE 6 QUARTER 3 REVIEWER(FRICTION, GRAVITY, ENERGY AND SPEED).pptx
SCIENCE 6 QUARTER 3 REVIEWER(FRICTION, GRAVITY, ENERGY AND SPEED).pptxROVELYNEDELUNA3
 
Human brain.. It's parts and function.
Human brain.. It's parts and function. Human brain.. It's parts and function.
Human brain.. It's parts and function. MUKTA MANJARI SAHOO
 
M.Pharm - Question Bank - Drug Delivery Systems
M.Pharm - Question Bank - Drug Delivery SystemsM.Pharm - Question Bank - Drug Delivery Systems
M.Pharm - Question Bank - Drug Delivery SystemsSumathi Arumugam
 
3.2 Pests of Sorghum_Identification, Symptoms and nature of damage, Binomics,...
3.2 Pests of Sorghum_Identification, Symptoms and nature of damage, Binomics,...3.2 Pests of Sorghum_Identification, Symptoms and nature of damage, Binomics,...
3.2 Pests of Sorghum_Identification, Symptoms and nature of damage, Binomics,...PirithiRaju
 
Substances in Common Use for Shahu College Screening Test
Substances in Common Use for Shahu College Screening TestSubstances in Common Use for Shahu College Screening Test
Substances in Common Use for Shahu College Screening TestAkashDTejwani
 
Digitized Continuous Magnetic Recordings for the August/September 1859 Storms...
Digitized Continuous Magnetic Recordings for the August/September 1859 Storms...Digitized Continuous Magnetic Recordings for the August/September 1859 Storms...
Digitized Continuous Magnetic Recordings for the August/September 1859 Storms...Sérgio Sacani
 
Pests of Redgram_Identification, Binomics_Dr.UPR
Pests of Redgram_Identification, Binomics_Dr.UPRPests of Redgram_Identification, Binomics_Dr.UPR
Pests of Redgram_Identification, Binomics_Dr.UPRPirithiRaju
 
Alternative system of medicine herbal drug technology syllabus
Alternative system of medicine herbal drug technology syllabusAlternative system of medicine herbal drug technology syllabus
Alternative system of medicine herbal drug technology syllabusPradnya Wadekar
 
Q3W4part1-SSSSSSSSSSSSSSSSSSSSSSSSCI.pptx
Q3W4part1-SSSSSSSSSSSSSSSSSSSSSSSSCI.pptxQ3W4part1-SSSSSSSSSSSSSSSSSSSSSSSSCI.pptx
Q3W4part1-SSSSSSSSSSSSSSSSSSSSSSSSCI.pptxArdeniel
 
Pests of ragi_Identification, Binomics_Dr.UPR
Pests of ragi_Identification, Binomics_Dr.UPRPests of ragi_Identification, Binomics_Dr.UPR
Pests of ragi_Identification, Binomics_Dr.UPRPirithiRaju
 
Minimal Residual Disease in leukaemia andhematological malignancies
Minimal Residual Disease in leukaemia andhematological malignanciesMinimal Residual Disease in leukaemia andhematological malignancies
Minimal Residual Disease in leukaemia andhematological malignanciessadiya97
 

Último (20)

Isabelle Diacaire - From Ariadnas to Industry R&D in optics and photonics
Isabelle Diacaire - From Ariadnas to Industry R&D in optics and photonicsIsabelle Diacaire - From Ariadnas to Industry R&D in optics and photonics
Isabelle Diacaire - From Ariadnas to Industry R&D in optics and photonics
 
World Water Day 22 March 2024 - kiyorndlab
World Water Day 22 March 2024 - kiyorndlabWorld Water Day 22 March 2024 - kiyorndlab
World Water Day 22 March 2024 - kiyorndlab
 
TORSION IN GASTROPODS- Anatomical event (Zoology)
TORSION IN GASTROPODS- Anatomical event (Zoology)TORSION IN GASTROPODS- Anatomical event (Zoology)
TORSION IN GASTROPODS- Anatomical event (Zoology)
 
Breast Cancer Pharmacology Presentation - Louis Pearce.pptx
Breast Cancer Pharmacology Presentation - Louis Pearce.pptxBreast Cancer Pharmacology Presentation - Louis Pearce.pptx
Breast Cancer Pharmacology Presentation - Louis Pearce.pptx
 
Physics Serway Jewett 6th edition for Scientists and Engineers
Physics Serway Jewett 6th edition for Scientists and EngineersPhysics Serway Jewett 6th edition for Scientists and Engineers
Physics Serway Jewett 6th edition for Scientists and Engineers
 
SUKDANAN DIAGNOSTIC TEST IN PHYSICAL SCIENCE ANSWER KEYY.pdf
SUKDANAN DIAGNOSTIC TEST IN PHYSICAL SCIENCE ANSWER KEYY.pdfSUKDANAN DIAGNOSTIC TEST IN PHYSICAL SCIENCE ANSWER KEYY.pdf
SUKDANAN DIAGNOSTIC TEST IN PHYSICAL SCIENCE ANSWER KEYY.pdf
 
Applying Cheminformatics to Develop a Structure Searchable Database of Analyt...
Applying Cheminformatics to Develop a Structure Searchable Database of Analyt...Applying Cheminformatics to Develop a Structure Searchable Database of Analyt...
Applying Cheminformatics to Develop a Structure Searchable Database of Analyt...
 
RCPE terms and cycles scenarios as of March 2024
RCPE terms and cycles scenarios as of March 2024RCPE terms and cycles scenarios as of March 2024
RCPE terms and cycles scenarios as of March 2024
 
SCIENCE 6 QUARTER 3 REVIEWER(FRICTION, GRAVITY, ENERGY AND SPEED).pptx
SCIENCE 6 QUARTER 3 REVIEWER(FRICTION, GRAVITY, ENERGY AND SPEED).pptxSCIENCE 6 QUARTER 3 REVIEWER(FRICTION, GRAVITY, ENERGY AND SPEED).pptx
SCIENCE 6 QUARTER 3 REVIEWER(FRICTION, GRAVITY, ENERGY AND SPEED).pptx
 
Human brain.. It's parts and function.
Human brain.. It's parts and function. Human brain.. It's parts and function.
Human brain.. It's parts and function.
 
M.Pharm - Question Bank - Drug Delivery Systems
M.Pharm - Question Bank - Drug Delivery SystemsM.Pharm - Question Bank - Drug Delivery Systems
M.Pharm - Question Bank - Drug Delivery Systems
 
3.2 Pests of Sorghum_Identification, Symptoms and nature of damage, Binomics,...
3.2 Pests of Sorghum_Identification, Symptoms and nature of damage, Binomics,...3.2 Pests of Sorghum_Identification, Symptoms and nature of damage, Binomics,...
3.2 Pests of Sorghum_Identification, Symptoms and nature of damage, Binomics,...
 
Cheminformatics tools supporting dissemination of data associated with US EPA...
Cheminformatics tools supporting dissemination of data associated with US EPA...Cheminformatics tools supporting dissemination of data associated with US EPA...
Cheminformatics tools supporting dissemination of data associated with US EPA...
 
Substances in Common Use for Shahu College Screening Test
Substances in Common Use for Shahu College Screening TestSubstances in Common Use for Shahu College Screening Test
Substances in Common Use for Shahu College Screening Test
 
Digitized Continuous Magnetic Recordings for the August/September 1859 Storms...
Digitized Continuous Magnetic Recordings for the August/September 1859 Storms...Digitized Continuous Magnetic Recordings for the August/September 1859 Storms...
Digitized Continuous Magnetic Recordings for the August/September 1859 Storms...
 
Pests of Redgram_Identification, Binomics_Dr.UPR
Pests of Redgram_Identification, Binomics_Dr.UPRPests of Redgram_Identification, Binomics_Dr.UPR
Pests of Redgram_Identification, Binomics_Dr.UPR
 
Alternative system of medicine herbal drug technology syllabus
Alternative system of medicine herbal drug technology syllabusAlternative system of medicine herbal drug technology syllabus
Alternative system of medicine herbal drug technology syllabus
 
Q3W4part1-SSSSSSSSSSSSSSSSSSSSSSSSCI.pptx
Q3W4part1-SSSSSSSSSSSSSSSSSSSSSSSSCI.pptxQ3W4part1-SSSSSSSSSSSSSSSSSSSSSSSSCI.pptx
Q3W4part1-SSSSSSSSSSSSSSSSSSSSSSSSCI.pptx
 
Pests of ragi_Identification, Binomics_Dr.UPR
Pests of ragi_Identification, Binomics_Dr.UPRPests of ragi_Identification, Binomics_Dr.UPR
Pests of ragi_Identification, Binomics_Dr.UPR
 
Minimal Residual Disease in leukaemia andhematological malignancies
Minimal Residual Disease in leukaemia andhematological malignanciesMinimal Residual Disease in leukaemia andhematological malignancies
Minimal Residual Disease in leukaemia andhematological malignancies
 

Trp operon

  • 1. trp OPERON Amal george MSc Biochemistry TRAP
  • 2. OPERON In genetics, an operon is a functioning unit of genomic DNA containing a cluster of genes under the control of a single promoter Promoter sequences - DNA sequences that define where transcription of a gene by RNA polymerase begins Operator - a segment of DNA to which a transcription factor binds to regulate gene expression by repressing it Structural genes - genes that codes for any RNA or protein product other than a regulatory factor
  • 3. HISTORY Discovered in 1953 by Jacques Monod and colleagues The trp operon in E. coli was the first repressible operon to be discovered Jacques Lucien Monod Negative regulatory operon
  • 4. trp OPERON • group of genes that transcribed, together&codes for the components for production of tryptophan
  • 5. trp OPERON - structure trp Operon Gene Gene Function P/O Promoter; operator sequence is found in the promoter trp L Leader sequence; attenuator (A) sequence is found in the leader trp E Gene for anthranilate synthetase subunit1 trp D Gene for anthranilate synthetase subunit2 trp C Gene for glycerolphosphate synthetase trp B Gene for tryptophan synthetase subunit1 trp A Gene for tryptophan synthetase subunit
  • 7. trp-repressible operon The binding of the effector molecule to the repressor greatly increases the affinity of repressor for the operator and the repressor binds and stops transcription. A regulator gene, regulator, or regulatory gene is a gene encodes repressor protein
  • 8. Corepressor Corepressor is a substance that inhibits the expression of genes Repressor proteins can be DNA- or RNA-binding: DNA-binding repressors - block the binding of RNA polymerase to the promoter
  • 11. Regulation – trp operon The trp operon is controlled by both * repressor protein binding to the operator region *translation-induced transcriptional attenuation
  • 12. Repression tryptophan is present in the environment tryptophan is absent in the environment 1 2
  • 13. Attenuation Transcriptional attenuation is a regulatory mechanism that causes premature termination of transcription under certain conditions 21
  • 15. TRAP tryptophan mediates transcription and translation of the genes responsible for its biosynthesis. Tryptophan accomplishes this through interactions with a regulatory protein known as tryptophan-activated RNA binding attenuation protein (TRAP).
  • 16. Thank you. Trp -repressor binding to operator DNATrp-repressor dimer