SlideShare una empresa de Scribd logo
1 de 8
Descargar para leer sin conexión
WASHED CELL SUSPENSIONS
Red cell suspensions provide the appropriate
serum to cell ratio to allow for grading and
interpretation of tests results
3% Red Blood Cell Concentration in Saline
The purpose of washing the red blood cells is to remove plasma,
which contains substance that may interfere with antigen-
reaction. The following may be in the plasma and may interfere
testing:
• Soluble antigens such as A and B may be present and neutralize
your reagent.
• Interfering proteins such as Wharton's jelly that is seen in
newborn cord blood, cold-acting autoimmune antibodies, and
increased levels of immunoglobulins that may cause either
agglutination or rouleaux..
• Hemolyzed red blood cells due to a difficult draw will interfere in
your grading interpretation of hemolysis
• Fibrinogen can result in fibrin strands forming that makes
Washing Red Blood Cells Before Making the 3% Suspension
In the process of blood typing, blood samples are mixed with
known anti-A and anti-B antibody serum, and are then
monitored for agglutination.
If agglutination occurs, then the particularABOantigen(s) on
the red blood cell surface have reacted with the antibodies
present in the serum.
Wharton’s jelly [ contain hyaluronic acid & chondroitin
can contaminate blood samples by coating the red blood cells
making the cells polyagglutinable, leading to a false positive
reaction.
When possible, cord blood should be collected using the
syringe method rather than the gravity and glass tube method
WHARTON’S JELLY
SPECIMEN
The preferred specimen is an EDTA (lavender top) tube of venous
blood.
Un-anticoagulated glass (red top) tubes may be used if
completely clotted. Prior to preparation of a cell suspension, the
specimens should be centrifuged except cord blood specimens and
specimens incorrectly provided in “serum separator” tubes. Patient
plasma/serum is separated from the red cells and placed into an
appropriately labeled tube with the patient's name and medical record
number or date of birth, and the date the sample was drawn.
RBCs are removed directly from cord blood specimens without centrifugation. For
serum separator tubes, remove RBCs prior to centrifugation and wash them as
Washed 3-5% patient or donor red cell suspension for tube tests starting with
packed cells
1. Properly label a 12 x 75 test tube.
2. With a pipette, remove approximately 3-5 drops of blood from the patient or
donor sample and add to the test tube. For donor tubing segments, cut open
the segment and squeeze 1-2 drops of blood into the test tube.
3. NOTE: Since antibody coated RBCs are heavier than uncoated cells
and settle to the bottom of the sample the contents should be mixed well before blood
is removed. If a clotted specimen is used, blood should be removed from the bottom of
the clot for the same reason.
4. Fill the test tube approximately 3/4 full with saline by positioning the tip of
the wash bottle directly over the tube and squeezing it. This will mix the blood
and the saline, increasing the efficiency of washing.
5. Centrifuge the tube for the appropriate time calibrated for cell washing.
6. When the centrifuge stops, remove the test tube and decant the supernatant
saline.
7. Shake the cells loose from the bottom of the tube.
8. Add the volume of saline necessary to prepare 3 5% suspension,
approximately 40 drops.
Method to defibrinate a clot
Materials
1. Sieve and pestle
2. Clotted blood specimen
Procedure
1. Spin the specimen until a good separation occurs between the clot and serum (3,500 rpm for 5 minutes).
2. Pipette the serum into a clean test tube labeled with the patient's name, account number, and date of the
sample.
3. Place the sieve over a Petri dish.
4. Pour the remaining clot onto the sieve and mash well with the pestle. Rinse the sieve with saline.
5. Transfer red cells suspended in saline from the Petri dish into clean 13x100 mm test tubes labeled with
patient's name and date of sample. Rinse the Petri dish and transfer the saline to the labeled tubes.
6. Spin these tubes at 3,500 rpm for l minute. Aspirate the supernatant saline and pool the packed cells, rinsing
remaining cells from the tubes with saline.
7. Continue pooling and centrifugation until all the cells are contained in one test tube.
8. Wash the cells until the supernatant is free of hemoglobin.
https://www.youtube.com/watch?v=cx1Gd5Gov0g
http://www.health.gov.nl.ca/health/bloodservices/pdf/prep_red_cell_susp.pdf
https://www.google.co.in/url?sa=t&rct=j&q=&esrc=s&source=web&cd=18&cad=rja&uact=
8&ved=0ahUKEwj7nJ_dwt7YAhVEoZQKHdXtDhYQFgh-
MBE&url=http%3A%2F%2Fwww.indianinitiative.org%2Fwp-
content%2Fuploads%2F2011%2F06%2F202-Preparation-of-Red-Blood-Cells-for-
Analysis.doc&usg=AOvVaw2Dk9RTrbpHd9nrqz_EFXGm
http://faculty.madisoncollege.edu/mljensen/BloodBank/lectur
es/Basic_Laboratory_techniques&Reagents.htm

Más contenido relacionado

La actualidad más candente

La actualidad más candente (20)

Reticulocyte count
Reticulocyte countReticulocyte count
Reticulocyte count
 
Osmotic fragility test
Osmotic fragility testOsmotic fragility test
Osmotic fragility test
 
Anticoagulants and blood preservatives
Anticoagulants and blood preservativesAnticoagulants and blood preservatives
Anticoagulants and blood preservatives
 
Blood component preparation
Blood component preparationBlood component preparation
Blood component preparation
 
Microscopic examination of urine
Microscopic examination of urineMicroscopic examination of urine
Microscopic examination of urine
 
morphology of red blood cells
morphology of red blood cellsmorphology of red blood cells
morphology of red blood cells
 
Final ppt sickle cell
Final ppt sickle cellFinal ppt sickle cell
Final ppt sickle cell
 
LABORATORY INVESTIGATION OF TRANSFUSION REACTION CASES
LABORATORY INVESTIGATION OF TRANSFUSION REACTION CASESLABORATORY INVESTIGATION OF TRANSFUSION REACTION CASES
LABORATORY INVESTIGATION OF TRANSFUSION REACTION CASES
 
Le cell
Le cellLe cell
Le cell
 
Blood component separation
Blood component separationBlood component separation
Blood component separation
 
COOMBS TEST.pptx
COOMBS TEST.pptxCOOMBS TEST.pptx
COOMBS TEST.pptx
 
Blood bank equipments ss
Blood bank equipments ssBlood bank equipments ss
Blood bank equipments ss
 
Reticulocyte count
Reticulocyte countReticulocyte count
Reticulocyte count
 
Gel card technology ppt nc
Gel card technology ppt ncGel card technology ppt nc
Gel card technology ppt nc
 
Osmotic Fragility Test
Osmotic Fragility TestOsmotic Fragility Test
Osmotic Fragility Test
 
Apheresis
ApheresisApheresis
Apheresis
 
Gel tech
Gel techGel tech
Gel tech
 
Osmotic fragility test
Osmotic fragility testOsmotic fragility test
Osmotic fragility test
 
Reticulocyte count
Reticulocyte countReticulocyte count
Reticulocyte count
 
Compatability testing
Compatability testingCompatability testing
Compatability testing
 

Similar a Making a 3 5% rbc suspension

Blood Group Systems_ABO & Rh.ppt
Blood Group Systems_ABO & Rh.pptBlood Group Systems_ABO & Rh.ppt
Blood Group Systems_ABO & Rh.pptssuser995ddb
 
Chapter 7-The cross-matching-1.ppt
Chapter 7-The cross-matching-1.pptChapter 7-The cross-matching-1.ppt
Chapter 7-The cross-matching-1.pptbesufkaddesta
 
Biomedical Instrumentation
Biomedical InstrumentationBiomedical Instrumentation
Biomedical Instrumentationlinsstalex
 
immunohema7.pptx
immunohema7.pptximmunohema7.pptx
immunohema7.pptxAdugnaWari
 
BLOOD GROUPING.pptx FOR DMLT BLOOD BANKING & HEMATOLOGY
BLOOD GROUPING.pptx FOR DMLT BLOOD BANKING & HEMATOLOGYBLOOD GROUPING.pptx FOR DMLT BLOOD BANKING & HEMATOLOGY
BLOOD GROUPING.pptx FOR DMLT BLOOD BANKING & HEMATOLOGYPunamSahoo3
 
Rational-Use-of-Blood-Blood-administration.pptx
Rational-Use-of-Blood-Blood-administration.pptxRational-Use-of-Blood-Blood-administration.pptx
Rational-Use-of-Blood-Blood-administration.pptxVaishaliNagpalYU
 
Blood componentsin transfusion medicine.pdf
Blood componentsin transfusion medicine.pdfBlood componentsin transfusion medicine.pdf
Blood componentsin transfusion medicine.pdfAmany Elshamy
 
safebloodtransfusion-170515065006.pdf
safebloodtransfusion-170515065006.pdfsafebloodtransfusion-170515065006.pdf
safebloodtransfusion-170515065006.pdfConstance39
 
Hemocytometer manual cell counting (1)
Hemocytometer manual cell counting (1)Hemocytometer manual cell counting (1)
Hemocytometer manual cell counting (1)Shabab Ali
 
Blood component – Principles of separation & indication.pptx
Blood component – Principles of separation & indication.pptxBlood component – Principles of separation & indication.pptx
Blood component – Principles of separation & indication.pptxssuser995ddb
 
Classification of Thrombocyte Disorders Using Platelet Aggregation Assays
Classification of Thrombocyte Disorders Using Platelet Aggregation AssaysClassification of Thrombocyte Disorders Using Platelet Aggregation Assays
Classification of Thrombocyte Disorders Using Platelet Aggregation AssaysChristine Joyce Javier
 

Similar a Making a 3 5% rbc suspension (20)

Blood bank
Blood bankBlood bank
Blood bank
 
blood grouping.ppt
blood grouping.pptblood grouping.ppt
blood grouping.ppt
 
Blood Group Systems_ABO & Rh.ppt
Blood Group Systems_ABO & Rh.pptBlood Group Systems_ABO & Rh.ppt
Blood Group Systems_ABO & Rh.ppt
 
Chapter 7-The cross-matching-1.ppt
Chapter 7-The cross-matching-1.pptChapter 7-The cross-matching-1.ppt
Chapter 7-The cross-matching-1.ppt
 
Blood bank
Blood bankBlood bank
Blood bank
 
Biomedical Instrumentation
Biomedical InstrumentationBiomedical Instrumentation
Biomedical Instrumentation
 
Compatability testing.pptx
Compatability testing.pptxCompatability testing.pptx
Compatability testing.pptx
 
immunohema7.pptx
immunohema7.pptximmunohema7.pptx
immunohema7.pptx
 
BLOOD GROUPING.pptx FOR DMLT BLOOD BANKING & HEMATOLOGY
BLOOD GROUPING.pptx FOR DMLT BLOOD BANKING & HEMATOLOGYBLOOD GROUPING.pptx FOR DMLT BLOOD BANKING & HEMATOLOGY
BLOOD GROUPING.pptx FOR DMLT BLOOD BANKING & HEMATOLOGY
 
Chapter 2.ppt
Chapter 2.pptChapter 2.ppt
Chapter 2.ppt
 
Compatibility ss
Compatibility ssCompatibility ss
Compatibility ss
 
Rational-Use-of-Blood-Blood-administration.pptx
Rational-Use-of-Blood-Blood-administration.pptxRational-Use-of-Blood-Blood-administration.pptx
Rational-Use-of-Blood-Blood-administration.pptx
 
Blood componentsin transfusion medicine.pdf
Blood componentsin transfusion medicine.pdfBlood componentsin transfusion medicine.pdf
Blood componentsin transfusion medicine.pdf
 
Safe Blood Transfusion
Safe Blood TransfusionSafe Blood Transfusion
Safe Blood Transfusion
 
safebloodtransfusion-170515065006.pdf
safebloodtransfusion-170515065006.pdfsafebloodtransfusion-170515065006.pdf
safebloodtransfusion-170515065006.pdf
 
Hemocytometer manual cell counting (1)
Hemocytometer manual cell counting (1)Hemocytometer manual cell counting (1)
Hemocytometer manual cell counting (1)
 
Blood component – Principles of separation & indication.pptx
Blood component – Principles of separation & indication.pptxBlood component – Principles of separation & indication.pptx
Blood component – Principles of separation & indication.pptx
 
Classification of Thrombocyte Disorders Using Platelet Aggregation Assays
Classification of Thrombocyte Disorders Using Platelet Aggregation AssaysClassification of Thrombocyte Disorders Using Platelet Aggregation Assays
Classification of Thrombocyte Disorders Using Platelet Aggregation Assays
 
Hemocytometry
Hemocytometry Hemocytometry
Hemocytometry
 
ABO blood.pptx
ABO blood.pptxABO blood.pptx
ABO blood.pptx
 

Más de Harshal Shinde

Loose connective tissue
Loose connective tissueLoose connective tissue
Loose connective tissueHarshal Shinde
 
Hot cross bun appearance
Hot  cross bun appearanceHot  cross bun appearance
Hot cross bun appearanceHarshal Shinde
 
Animals that are exotic and look alike
Animals that are exotic and look alikeAnimals that are exotic and look alike
Animals that are exotic and look alikeHarshal Shinde
 
Brachial artery,radial & ulnar artery,plamar arches
Brachial artery,radial & ulnar artery,plamar archesBrachial artery,radial & ulnar artery,plamar arches
Brachial artery,radial & ulnar artery,plamar archesHarshal Shinde
 
Subclavian artery and it's branches
Subclavian artery and it's branchesSubclavian artery and it's branches
Subclavian artery and it's branchesHarshal Shinde
 
Forearm muscles anterior (flexor) compartment
Forearm muscles anterior (flexor) compartmentForearm muscles anterior (flexor) compartment
Forearm muscles anterior (flexor) compartmentHarshal Shinde
 
Tennis elbow & Golfer's elbow
Tennis elbow & Golfer's elbowTennis elbow & Golfer's elbow
Tennis elbow & Golfer's elbowHarshal Shinde
 
Physics of Sound-Inside view
Physics of Sound-Inside viewPhysics of Sound-Inside view
Physics of Sound-Inside viewHarshal Shinde
 

Más de Harshal Shinde (15)

Assessment questions
Assessment questionsAssessment questions
Assessment questions
 
Arcuate line
Arcuate lineArcuate line
Arcuate line
 
Loose connective tissue
Loose connective tissueLoose connective tissue
Loose connective tissue
 
Hot cross bun appearance
Hot  cross bun appearanceHot  cross bun appearance
Hot cross bun appearance
 
Animals that are exotic and look alike
Animals that are exotic and look alikeAnimals that are exotic and look alike
Animals that are exotic and look alike
 
Brachial artery,radial & ulnar artery,plamar arches
Brachial artery,radial & ulnar artery,plamar archesBrachial artery,radial & ulnar artery,plamar arches
Brachial artery,radial & ulnar artery,plamar arches
 
Subclavian artery and it's branches
Subclavian artery and it's branchesSubclavian artery and it's branches
Subclavian artery and it's branches
 
Ulnar nerve
Ulnar nerveUlnar nerve
Ulnar nerve
 
Forearm muscles anterior (flexor) compartment
Forearm muscles anterior (flexor) compartmentForearm muscles anterior (flexor) compartment
Forearm muscles anterior (flexor) compartment
 
Tennis elbow & Golfer's elbow
Tennis elbow & Golfer's elbowTennis elbow & Golfer's elbow
Tennis elbow & Golfer's elbow
 
Elbow joint anatomy
Elbow joint anatomyElbow joint anatomy
Elbow joint anatomy
 
Physics of Sound-Inside view
Physics of Sound-Inside viewPhysics of Sound-Inside view
Physics of Sound-Inside view
 
Hemolysis
HemolysisHemolysis
Hemolysis
 
Syndrome
SyndromeSyndrome
Syndrome
 
Baby manifesto
Baby manifestoBaby manifesto
Baby manifesto
 

Último

Clinical Research Informatics Year-in-Review 2024
Clinical Research Informatics Year-in-Review 2024Clinical Research Informatics Year-in-Review 2024
Clinical Research Informatics Year-in-Review 2024Peter Embi
 
ORAL HYPOGLYCAEMIC AGENTS - PART 2.pptx
ORAL HYPOGLYCAEMIC AGENTS  - PART 2.pptxORAL HYPOGLYCAEMIC AGENTS  - PART 2.pptx
ORAL HYPOGLYCAEMIC AGENTS - PART 2.pptxNIKITA BHUTE
 
MedMatch: Your Health, Our Mission. Pitch deck.
MedMatch: Your Health, Our Mission. Pitch deck.MedMatch: Your Health, Our Mission. Pitch deck.
MedMatch: Your Health, Our Mission. Pitch deck.whalesdesign
 
Female Reproductive Physiology Before Pregnancy
Female Reproductive Physiology Before PregnancyFemale Reproductive Physiology Before Pregnancy
Female Reproductive Physiology Before PregnancyMedicoseAcademics
 
"Radical excision of DIE in subferile women with deep infiltrating endometrio...
"Radical excision of DIE in subferile women with deep infiltrating endometrio..."Radical excision of DIE in subferile women with deep infiltrating endometrio...
"Radical excision of DIE in subferile women with deep infiltrating endometrio...Sujoy Dasgupta
 
SGK NGẠT NƯỚC ĐHYHN RẤT LÀ HAY NHA .pdf
SGK NGẠT NƯỚC ĐHYHN RẤT LÀ HAY NHA    .pdfSGK NGẠT NƯỚC ĐHYHN RẤT LÀ HAY NHA    .pdf
SGK NGẠT NƯỚC ĐHYHN RẤT LÀ HAY NHA .pdfHongBiThi1
 
Trustworthiness of AI based predictions Aachen 2024
Trustworthiness of AI based predictions Aachen 2024Trustworthiness of AI based predictions Aachen 2024
Trustworthiness of AI based predictions Aachen 2024EwoutSteyerberg1
 
DNA nucleotides Blast in NCBI and Phylogeny using MEGA Xi.pptx
DNA nucleotides Blast in NCBI and Phylogeny using MEGA Xi.pptxDNA nucleotides Blast in NCBI and Phylogeny using MEGA Xi.pptx
DNA nucleotides Blast in NCBI and Phylogeny using MEGA Xi.pptxMAsifAhmad
 
How to cure cirrhosis and chronic hepatitis naturally
How to cure cirrhosis and chronic hepatitis naturallyHow to cure cirrhosis and chronic hepatitis naturally
How to cure cirrhosis and chronic hepatitis naturallyZurück zum Ursprung
 
pA2 value, Schild plot and pD2 values- applications in pharmacology
pA2 value, Schild plot and pD2 values- applications in pharmacologypA2 value, Schild plot and pD2 values- applications in pharmacology
pA2 value, Schild plot and pD2 values- applications in pharmacologyDeepakDaniel9
 
SGK RỐI LOẠN TOAN KIỀM ĐHYHN RẤT HAY VÀ ĐẶC SẮC.pdf
SGK RỐI LOẠN TOAN KIỀM ĐHYHN RẤT HAY VÀ ĐẶC SẮC.pdfSGK RỐI LOẠN TOAN KIỀM ĐHYHN RẤT HAY VÀ ĐẶC SẮC.pdf
SGK RỐI LOẠN TOAN KIỀM ĐHYHN RẤT HAY VÀ ĐẶC SẮC.pdfHongBiThi1
 
Breast cancer -ONCO IN MEDICAL AND SURGICAL NURSING.pptx
Breast cancer -ONCO IN MEDICAL AND SURGICAL NURSING.pptxBreast cancer -ONCO IN MEDICAL AND SURGICAL NURSING.pptx
Breast cancer -ONCO IN MEDICAL AND SURGICAL NURSING.pptxNaveenkumar267201
 
AORTIC DISSECTION and management of aortic dissection
AORTIC DISSECTION and management of aortic dissectionAORTIC DISSECTION and management of aortic dissection
AORTIC DISSECTION and management of aortic dissectiondrhanifmohdali
 
AUTONOMIC NERVOUS SYSTEM organization and functions
AUTONOMIC NERVOUS SYSTEM organization and functionsAUTONOMIC NERVOUS SYSTEM organization and functions
AUTONOMIC NERVOUS SYSTEM organization and functionsMedicoseAcademics
 
Basic structure of hair and hair growth cycle.pptx
Basic structure of hair and hair growth cycle.pptxBasic structure of hair and hair growth cycle.pptx
Basic structure of hair and hair growth cycle.pptxkomalt2001
 
EXERCISE PERFORMANCE.pptx, Lung function
EXERCISE PERFORMANCE.pptx, Lung functionEXERCISE PERFORMANCE.pptx, Lung function
EXERCISE PERFORMANCE.pptx, Lung functionkrishnareddy157915
 
Different drug regularity bodies in different countries.
Different drug regularity bodies in different countries.Different drug regularity bodies in different countries.
Different drug regularity bodies in different countries.kishan singh tomar
 

Último (20)

Clinical Research Informatics Year-in-Review 2024
Clinical Research Informatics Year-in-Review 2024Clinical Research Informatics Year-in-Review 2024
Clinical Research Informatics Year-in-Review 2024
 
ORAL HYPOGLYCAEMIC AGENTS - PART 2.pptx
ORAL HYPOGLYCAEMIC AGENTS  - PART 2.pptxORAL HYPOGLYCAEMIC AGENTS  - PART 2.pptx
ORAL HYPOGLYCAEMIC AGENTS - PART 2.pptx
 
MedMatch: Your Health, Our Mission. Pitch deck.
MedMatch: Your Health, Our Mission. Pitch deck.MedMatch: Your Health, Our Mission. Pitch deck.
MedMatch: Your Health, Our Mission. Pitch deck.
 
Female Reproductive Physiology Before Pregnancy
Female Reproductive Physiology Before PregnancyFemale Reproductive Physiology Before Pregnancy
Female Reproductive Physiology Before Pregnancy
 
"Radical excision of DIE in subferile women with deep infiltrating endometrio...
"Radical excision of DIE in subferile women with deep infiltrating endometrio..."Radical excision of DIE in subferile women with deep infiltrating endometrio...
"Radical excision of DIE in subferile women with deep infiltrating endometrio...
 
SGK NGẠT NƯỚC ĐHYHN RẤT LÀ HAY NHA .pdf
SGK NGẠT NƯỚC ĐHYHN RẤT LÀ HAY NHA    .pdfSGK NGẠT NƯỚC ĐHYHN RẤT LÀ HAY NHA    .pdf
SGK NGẠT NƯỚC ĐHYHN RẤT LÀ HAY NHA .pdf
 
Trustworthiness of AI based predictions Aachen 2024
Trustworthiness of AI based predictions Aachen 2024Trustworthiness of AI based predictions Aachen 2024
Trustworthiness of AI based predictions Aachen 2024
 
DNA nucleotides Blast in NCBI and Phylogeny using MEGA Xi.pptx
DNA nucleotides Blast in NCBI and Phylogeny using MEGA Xi.pptxDNA nucleotides Blast in NCBI and Phylogeny using MEGA Xi.pptx
DNA nucleotides Blast in NCBI and Phylogeny using MEGA Xi.pptx
 
How to cure cirrhosis and chronic hepatitis naturally
How to cure cirrhosis and chronic hepatitis naturallyHow to cure cirrhosis and chronic hepatitis naturally
How to cure cirrhosis and chronic hepatitis naturally
 
pA2 value, Schild plot and pD2 values- applications in pharmacology
pA2 value, Schild plot and pD2 values- applications in pharmacologypA2 value, Schild plot and pD2 values- applications in pharmacology
pA2 value, Schild plot and pD2 values- applications in pharmacology
 
SGK RỐI LOẠN TOAN KIỀM ĐHYHN RẤT HAY VÀ ĐẶC SẮC.pdf
SGK RỐI LOẠN TOAN KIỀM ĐHYHN RẤT HAY VÀ ĐẶC SẮC.pdfSGK RỐI LOẠN TOAN KIỀM ĐHYHN RẤT HAY VÀ ĐẶC SẮC.pdf
SGK RỐI LOẠN TOAN KIỀM ĐHYHN RẤT HAY VÀ ĐẶC SẮC.pdf
 
Breast cancer -ONCO IN MEDICAL AND SURGICAL NURSING.pptx
Breast cancer -ONCO IN MEDICAL AND SURGICAL NURSING.pptxBreast cancer -ONCO IN MEDICAL AND SURGICAL NURSING.pptx
Breast cancer -ONCO IN MEDICAL AND SURGICAL NURSING.pptx
 
American College of physicians ACP high value care recommendations in rheumat...
American College of physicians ACP high value care recommendations in rheumat...American College of physicians ACP high value care recommendations in rheumat...
American College of physicians ACP high value care recommendations in rheumat...
 
AORTIC DISSECTION and management of aortic dissection
AORTIC DISSECTION and management of aortic dissectionAORTIC DISSECTION and management of aortic dissection
AORTIC DISSECTION and management of aortic dissection
 
Rheumatoid arthritis Part 1, case based approach with application of the late...
Rheumatoid arthritis Part 1, case based approach with application of the late...Rheumatoid arthritis Part 1, case based approach with application of the late...
Rheumatoid arthritis Part 1, case based approach with application of the late...
 
AUTONOMIC NERVOUS SYSTEM organization and functions
AUTONOMIC NERVOUS SYSTEM organization and functionsAUTONOMIC NERVOUS SYSTEM organization and functions
AUTONOMIC NERVOUS SYSTEM organization and functions
 
Basic structure of hair and hair growth cycle.pptx
Basic structure of hair and hair growth cycle.pptxBasic structure of hair and hair growth cycle.pptx
Basic structure of hair and hair growth cycle.pptx
 
EXERCISE PERFORMANCE.pptx, Lung function
EXERCISE PERFORMANCE.pptx, Lung functionEXERCISE PERFORMANCE.pptx, Lung function
EXERCISE PERFORMANCE.pptx, Lung function
 
Immune labs basics part 1 acute phase reactants ESR, CRP Ahmed Yehia Ismaeel,...
Immune labs basics part 1 acute phase reactants ESR, CRP Ahmed Yehia Ismaeel,...Immune labs basics part 1 acute phase reactants ESR, CRP Ahmed Yehia Ismaeel,...
Immune labs basics part 1 acute phase reactants ESR, CRP Ahmed Yehia Ismaeel,...
 
Different drug regularity bodies in different countries.
Different drug regularity bodies in different countries.Different drug regularity bodies in different countries.
Different drug regularity bodies in different countries.
 

Making a 3 5% rbc suspension

  • 2. Red cell suspensions provide the appropriate serum to cell ratio to allow for grading and interpretation of tests results 3% Red Blood Cell Concentration in Saline
  • 3. The purpose of washing the red blood cells is to remove plasma, which contains substance that may interfere with antigen- reaction. The following may be in the plasma and may interfere testing: • Soluble antigens such as A and B may be present and neutralize your reagent. • Interfering proteins such as Wharton's jelly that is seen in newborn cord blood, cold-acting autoimmune antibodies, and increased levels of immunoglobulins that may cause either agglutination or rouleaux.. • Hemolyzed red blood cells due to a difficult draw will interfere in your grading interpretation of hemolysis • Fibrinogen can result in fibrin strands forming that makes Washing Red Blood Cells Before Making the 3% Suspension
  • 4. In the process of blood typing, blood samples are mixed with known anti-A and anti-B antibody serum, and are then monitored for agglutination. If agglutination occurs, then the particularABOantigen(s) on the red blood cell surface have reacted with the antibodies present in the serum. Wharton’s jelly [ contain hyaluronic acid & chondroitin can contaminate blood samples by coating the red blood cells making the cells polyagglutinable, leading to a false positive reaction. When possible, cord blood should be collected using the syringe method rather than the gravity and glass tube method WHARTON’S JELLY
  • 5. SPECIMEN The preferred specimen is an EDTA (lavender top) tube of venous blood. Un-anticoagulated glass (red top) tubes may be used if completely clotted. Prior to preparation of a cell suspension, the specimens should be centrifuged except cord blood specimens and specimens incorrectly provided in “serum separator” tubes. Patient plasma/serum is separated from the red cells and placed into an appropriately labeled tube with the patient's name and medical record number or date of birth, and the date the sample was drawn. RBCs are removed directly from cord blood specimens without centrifugation. For serum separator tubes, remove RBCs prior to centrifugation and wash them as
  • 6. Washed 3-5% patient or donor red cell suspension for tube tests starting with packed cells 1. Properly label a 12 x 75 test tube. 2. With a pipette, remove approximately 3-5 drops of blood from the patient or donor sample and add to the test tube. For donor tubing segments, cut open the segment and squeeze 1-2 drops of blood into the test tube. 3. NOTE: Since antibody coated RBCs are heavier than uncoated cells and settle to the bottom of the sample the contents should be mixed well before blood is removed. If a clotted specimen is used, blood should be removed from the bottom of the clot for the same reason. 4. Fill the test tube approximately 3/4 full with saline by positioning the tip of the wash bottle directly over the tube and squeezing it. This will mix the blood and the saline, increasing the efficiency of washing. 5. Centrifuge the tube for the appropriate time calibrated for cell washing. 6. When the centrifuge stops, remove the test tube and decant the supernatant saline. 7. Shake the cells loose from the bottom of the tube. 8. Add the volume of saline necessary to prepare 3 5% suspension, approximately 40 drops.
  • 7. Method to defibrinate a clot Materials 1. Sieve and pestle 2. Clotted blood specimen Procedure 1. Spin the specimen until a good separation occurs between the clot and serum (3,500 rpm for 5 minutes). 2. Pipette the serum into a clean test tube labeled with the patient's name, account number, and date of the sample. 3. Place the sieve over a Petri dish. 4. Pour the remaining clot onto the sieve and mash well with the pestle. Rinse the sieve with saline. 5. Transfer red cells suspended in saline from the Petri dish into clean 13x100 mm test tubes labeled with patient's name and date of sample. Rinse the Petri dish and transfer the saline to the labeled tubes. 6. Spin these tubes at 3,500 rpm for l minute. Aspirate the supernatant saline and pool the packed cells, rinsing remaining cells from the tubes with saline. 7. Continue pooling and centrifugation until all the cells are contained in one test tube. 8. Wash the cells until the supernatant is free of hemoglobin.