Laboratory investigation of dengue in Jeddah

Laboratory Diagnosis of Dengue Hemorrhagic Fever Dr. Hosam H. A. Madani MBBS King Abdul-Aziz University 1987 Dip Bact Manchester University 1992 MsC Virology London university 1995 Jeddah Regional Laboratory.

Dengue Virus ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Laboratory Diagnosis of Dengue ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Jeddah Regional Laboratory ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Virus isolation ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Virus Isolation ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Virus isolation ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Antigen Detection in tissues ,[object Object],[object Object],[object Object]

Haemagglutination-Inhibition test ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Complement fixation test ,[object Object],[object Object],[object Object],[object Object]

Neutralization test ,[object Object],[object Object],[object Object]

Dot-blot immunoassay ,[object Object],[object Object]

IgM Elisa ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

IgG Elisa ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

IgM / IgG Elisa ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

IgM/IgG Elisa ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

IgM / IgG Elisa ,[object Object],[object Object],[object Object]

IgM Elisa in JRL ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

IgG Elisa in JRL ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

IgG in Secondary infection ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Why PCR? ,[object Object],[object Object],[object Object],[object Object]

Molecular Diagnostics of dengue ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

RNA extraction ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

RT-PCR ,[object Object],[object Object],[object Object],[object Object]

One step- RT-PCR ,[object Object],[object Object],[object Object],[object Object]

Probes for dengue Screening ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Probes for dengue typing D 4 TM D 3 TM Den 4 R Den 3 R Den 4 s Den 3 s Type 4 : Type 3 : D 2 TM D 1 TM Den 2 R Den 1 R Den 2 s Den 1 s Type 2 : Type 1:

Percentage of Positive results 59.40 1514 46.80 1193 12.59 321 2549 Total 20.97 13 20.97 13 0.00 0 62 Shawal 1427 37.04 20 33.33 18 3.70 2 54 Ramadan 1427 38.89 28 38.89 28 0.00 0 72 Shaban 1427 22.64 12 22.64 12 0.00 0 53 Rajab 1427 55.42 46 34.94 29 20.48 17 83 Jumad II 1427 69.89 253 45.03 163 24.86 90 362 Jumad I 1427 57.95 339 48.03 281 9.91 58 585 RabieII 1427 56.67 272 46.25 222 10.42 50 480 Rabie I 1427 66.14 295 51.57 230 14.57 65 446 Safar 1427 68.75 143 60.10 125 8.65 18 208 Muharam 1427 64.58 93 50.00 72 14.58 21 144 Zul Heja 1426 % Total % IgM % PCR Susbected Date

Reference ,[object Object],[object Object],[object Object],[object Object]

Laboratory investigation of dengue in Jeddah

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