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Mouse Mesenteric Lymph Node Transcriptome Profile And Allergic Reactions In Response To Common Food Allergens Treatment Mainul Husain Department of Animal & Poultry Science University of Guelph Ontario, Canada
Rationale ,[object Object],[object Object],[object Object],[object Object],[object Object]
Sensitization and Gene Expression ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Elicitation ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],2 Weeks Day 0 1st Sensitization of Mice by I.P. Injection Day 1 2nd Sensitization of Mice by I.P. Injection Day 16 Euthanization of  Mice After 24 Hours Observation Day 15 Challenge of Mice by Ear Stripping And 24 Hours Observation
Ear Swelling Response To  Allergen Challenge ,[object Object],[object Object],[object Object]
24 Hr. Serum IgG1 Response To  Allergen Challenge ,[object Object]
24 Hr. Serum IgE Response To  Allergen Challenge ,[object Object],[object Object]
Plasma Histamine Response to Allergen Challenge ,[object Object],[object Object]
cDNA Microarray ,[object Object],[object Object],[object Object]
cDNA Microarray ,[object Object],[object Object],[object Object]
Overlapping And Unique Genes from 3 Treatment Groups BLG Treatment Group (1361 Genes) OVA Treatment Groups (533 Genes) PNA Treatment Groups (488 Genes) 92 131 77 988 175 169
Gene Ontology (GO) Analysis 204 Significant (P<0.05) GO Biological Processes Enriched with the Genes from BLG Treatment Group 327 Significant (P<0.05) GO Biological Processes Enriched with the Genes from OVA Treatment Groups 296 Significant (P<0.05) GO Biological Processes Enriched with the Genes from PNA Treatment Groups 24 30 112 104 139 114
Gene Ontology (GO) Biological Processes  GO Biological Processes GO ID BLG Treatment PNA Treatment OVA Treatment Genes P-Value Genes P-Value Genes P-Value Response to stimulus 50896 100 0.005 50 0.002 50 0.011 Muscle development 7517 16 0.033 9 0.021 10 0.014 Cytokine and chemokine mediated signaling pathway 19221 10 0.000 5 0.006 4 0.038 Acute inflammatory response 2526 9 0.001 5 0.004 6 0.001 Humoral immune response 6959 8 0.001 5 0.002 6 0.001
Pathways Identified In Response to Known Allergen Treatment  ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
T-Cell Receptor Signaling Pathway
IL-7 Signal Transduction  Pathway
Real-Time RT-PCR ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Real-Time RT-PCR ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Real-Time RT-PCR ,[object Object],[object Object],[object Object],[object Object]
Conclusion ,[object Object],[object Object],[object Object]

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Mouse Model of Allergy

  • 1. Mouse Mesenteric Lymph Node Transcriptome Profile And Allergic Reactions In Response To Common Food Allergens Treatment Mainul Husain Department of Animal & Poultry Science University of Guelph Ontario, Canada
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  • 11. Overlapping And Unique Genes from 3 Treatment Groups BLG Treatment Group (1361 Genes) OVA Treatment Groups (533 Genes) PNA Treatment Groups (488 Genes) 92 131 77 988 175 169
  • 12. Gene Ontology (GO) Analysis 204 Significant (P<0.05) GO Biological Processes Enriched with the Genes from BLG Treatment Group 327 Significant (P<0.05) GO Biological Processes Enriched with the Genes from OVA Treatment Groups 296 Significant (P<0.05) GO Biological Processes Enriched with the Genes from PNA Treatment Groups 24 30 112 104 139 114
  • 13. Gene Ontology (GO) Biological Processes GO Biological Processes GO ID BLG Treatment PNA Treatment OVA Treatment Genes P-Value Genes P-Value Genes P-Value Response to stimulus 50896 100 0.005 50 0.002 50 0.011 Muscle development 7517 16 0.033 9 0.021 10 0.014 Cytokine and chemokine mediated signaling pathway 19221 10 0.000 5 0.006 4 0.038 Acute inflammatory response 2526 9 0.001 5 0.004 6 0.001 Humoral immune response 6959 8 0.001 5 0.002 6 0.001
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