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John B. Cole
Animal Genomics and Improvement Laboratory
Agricultural Research Service, USDA
Beltsville, MD 20705-2350
john.cole@ars.usda.gov
2015
If we would see further than
others: research & technology
today and tomorrow
50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (2) Cole
We all have our favorite technologies
50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (3) Cole
Benefits of technology
 Technologies provide benefits by
making our work…
 Faster – More outputs are produced
per unit of time.
 Cheaper – The cost of producing a
unit of output decreases.
 Easier – Tasks require less physical
or mental labor.
50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (4) Cole
This is the age of precision
 We now have technologies to monitor
what goes into and what comes out of
cows with great precision.
 Inputs and outputs are inextricably
linked.
 We want the highest quality available at
the possible lowest cost.
50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (5) Cole
Everything needs to support cows
http://commons.wikimedia.org/wiki/File:Amish_dairy_farm_3.jpg
Parlor: milk and milk solids are
the primary source of dairy farm
income
Pasture: provides nutrition and
supports animal welfare
Silo/bunker: cattle cannot
perform without high-quality
diets
Cow: the dairy cow is the
machine without which the farm
cannot function
Herdsmen/consultants: experts
ensure that cows have an optimal
environment in which to perform
Barn: provides a safe and health
habitat for animal production
50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (6) Cole
High technology on my first farm…
Source: http://seasonalontariofood.blogspot.com/2011/04/visit-to-wooldrift-farm.html.
50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (7) Cole
The dairy industry has been a leader
Source: http://vet.tufts.edu/tas/images/002.png.
Source: http//www.shopbrownswiss.com/.
50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (8) Cole
Feeding the dairy cow
Top: Automated system for
measuring feed intake.
Bottom: Automated feeding
system being installed at
Embrapa Gado de Leite.
50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (9) Cole
Watering the dairy cow
Top: Automated waterer
with scale for measuring
intake.
Bottom: Automated scale
that weighs cows at the
waterer.
50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (10) Cole
Monitoring the dairy cow
Source: http://support.smaxtec-animalcare.com/.
Source:http://c-lockinc.com/.
Source:http://www.afimilk.com/.
50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (11) Cole
Milking the dairy cow
Source: http://www.afimilk.com/.
Source: http://goo.gl/wu8YtR.
Source: http://www.afimilk.com/.
Manufacturers such as Afimilk and
DeLaval provide intelligent milk
meters, inline milk analysis
sensors (e.g., AfiLab), and herd
management systems (e.g., Herd
Navigator).
50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (12) Cole
…and innovation is ongoing
Photos courtesy of Albert de Vries.
The Swedish Agricultural
University dairy research center
has a state-of-the-art facility
equipped with the latest
DeLaval technology.
50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (13) Cole
What else comes out of the cow?
Environment chambers at
Embrapa Gado de Leite,
Coronel Pacheco, MG, Brasil.
50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (14) Cole
Other uses of on-farm technology
Left, middle: Dairies
in Germany and
Italy sell energy
from biogas plants
and rooftop solar
cells.
Right: A dairy in
Germany sells
fresh milk directly to
consumers from an
automated, on-farm
shop.
50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (15) Cole
But you don’t get something for nothing
 New technologies often require
considerable capital investment.
 They sometimes fail to work as
advertised, or do not deliver the
promised gain.
 The data are often most useful when
combined with observations from many
farms.
50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (16) Cole
What challenges are on the horizon?
 Monthly milk samples are too
infrequent for modern management.
 Many large farms do not see a value
proposition in milk recording.
 The amount of data collected on-farm
are growing, but they are not being
collected in a central database.
50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (17) Cole
Trait
Relative emphasis on traits in index (%)
NM$
1994
NM$
2000
NM$
2003
NM$
2006
NM$
2010
NM$
2014
GM$
2014
Milk 6 5 0 0 0 -1 -1
Fat 25 21 22 23 19 22 20
Protein 43 36 33 23 16 20 18
PL 20 14 11 17 22 19 10
SCS –6 –9 –9 –9 –10 –7 -6
UDC … 7 7 6 7 8 8
FLC … 4 4 3 4 3 3
BDC … –4 –3 –4 –6 –5 -4
DPR … … 7 9 11 7 19
HCR … … … … … 2 3
CCR … … … … … 1 5
CA$ … … 4 6 5 5 5
Our focus has changed over time
50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (18) Cole
New phenotypes should add information
low high
Genetic correlation with
existing traits
lowhigh
Phenotypiccorrelation
withexistingtraits
Novel phenotypes
include some
new information
Novel phenotypes
include much
new information
Novel phenotypes
contain some
new information
Novel phenotypes
contain little
new information
50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (19) Cole
What do current phenotypes look like?
 Low-dimensionality
 Usually few observations per lactation
 Close correspondence of phenotypes
with values measured
 Easy transmission and storage
50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (20) Cole
What do new phenotypes look like?
 High dimensionality
 Ex.: MIR produces 1,060 points/obs.
 Disconnect between phenotype and
measurement
 More resources needed for transmission,
storage, and analysis
50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (21) Cole
Name Chrome Location (Mbp) Freq of minor haplotype Gene Name
HH1 5 63.15 1.92 APAF1
HH2 1 94.8 to 96.6 1.66 unknown
HH3 8 95.41 2.95 SMC2
HH4 1 1.27 0.37 GART
HH5 9 92 to 94 2.22 unknown
JH1 15 15.70 12.10 CWC15
JH2 26 8.81 to 9.41 1.3 unknown
BH1 7 42.8 to 47.0 6.67 unknown
BH2 19 10.6 to 11.7 7.78 unknown
AH1 17 65.92 13.0 UBE3B
Phenotypes may come from genotypes
For a complete list, see: http://aipl.arsusda.gov/reference/recessive_haplotypes_ARR-G3.html.
50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (22) Cole
Genotypes in the national database
0
100000
200000
300000
400000
500000
600000
700000
800000
NumberofGenotypes
Run Date
Imputed, Young
Imputed, Old
<50k, Young, Female
<50k, Young, Male
<50k, Old, Female
<50k, Old, Male
50k, Young, Female
50k, Young, Male
50k, Old, Female
50k, Old, Male
50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (23) Cole
Genotyped ancestors, actual bull(HOUSA73431994)
777K 777K
50K - - -
50K 50K
50K 50K
777K 777K
50K - - -
3K 777K
50K 50K
777K 777K
50K Imputed
Imputed 50K
50K Imputed
50K 777K
50K 50K
50K 777K
77K - - -
777K 777K
50K Imputed
Imputed 50K
50K Imputed
50K 777K
50K 50K
3K 777K
9K 50K
50K 777K
50K 50K
50K 777K
50K Imputed
777K 777K
50K - - -
Imputed 777K
50K
Genotyped or imputed animals
Both parents
All 4 grandparents
All 8 great grandparents
All 16 great, great grandparents
28 of 32 great, great, great
grandparents
56 of 60 ancestors in pedigree
50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (24) Cole
Genome assembly (simplified)
Reads must be assembled into chromosomes
Assembly is a computational process (Liu et al., 2009; Zimin et al., 2009)
This process is imperfect – repetitive regions are hard to assemble correctly!
Sometimes, this…
should be this.
50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (25) Cole
Possible assembly problem on BTA18
This could be a GC-rich region (bias in
Illumina chemistry).
More reads than expected may align
here because repetitive elements were
combined during assembly.
50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (26) Cole
Can it be corrected using long reads?
• BTA18 genomic DNA extracted
from CHORI-240 BAC library
(L1 Domino 99375) at AGIL
• Sequencing libraries constructed at
USDA MARC, pooled, and run on PacBio
RS II
BAC ID Insert size (bp) Start End
CH240-389P14 174,682 56,954,654 57,129,335
CH240-234E12 178,618 57,058,248 57,236,865
CH240-280L6 175,831 57,092,237 57,268,067
CH240-34N7 158,841 57,129,383 57,288,223
Source: Pacific Biosystems
50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (27) Cole
Conclusions
 Modern sensor technology is routinely
producing large amounts of data.
 Those data have the potential to
improve herd management and
profitability.
 They can support development of new
management practices and research
into novel phenotypes.
50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (28) Cole
Acknowledgments
• AFRI Competitive Grant No 2013-68004-
20365, “Improving Fertility of Dairy Cattle
Using Translational Genomics”
• Cooperative Dairy DNA Repository
• Council on Dairy Cattle Breeding
• Paul VanRaden and George Wiggans, AGIL
• Albert de Vries, University of Florida
• Kent Weigel, University of Wisconsin
50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (29) Cole
Note
Mention of trade names or commercial
products in this presentation is solely for
the purpose of providing specific
information and does not imply
recommendation or endorsement by the
US Department of Agriculture.
50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (30) Cole
Questions?

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If we would see further than others: research & technology today and tomorrow

  • 1. John B. Cole Animal Genomics and Improvement Laboratory Agricultural Research Service, USDA Beltsville, MD 20705-2350 john.cole@ars.usda.gov 2015 If we would see further than others: research & technology today and tomorrow
  • 2. 50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (2) Cole We all have our favorite technologies
  • 3. 50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (3) Cole Benefits of technology  Technologies provide benefits by making our work…  Faster – More outputs are produced per unit of time.  Cheaper – The cost of producing a unit of output decreases.  Easier – Tasks require less physical or mental labor.
  • 4. 50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (4) Cole This is the age of precision  We now have technologies to monitor what goes into and what comes out of cows with great precision.  Inputs and outputs are inextricably linked.  We want the highest quality available at the possible lowest cost.
  • 5. 50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (5) Cole Everything needs to support cows http://commons.wikimedia.org/wiki/File:Amish_dairy_farm_3.jpg Parlor: milk and milk solids are the primary source of dairy farm income Pasture: provides nutrition and supports animal welfare Silo/bunker: cattle cannot perform without high-quality diets Cow: the dairy cow is the machine without which the farm cannot function Herdsmen/consultants: experts ensure that cows have an optimal environment in which to perform Barn: provides a safe and health habitat for animal production
  • 6. 50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (6) Cole High technology on my first farm… Source: http://seasonalontariofood.blogspot.com/2011/04/visit-to-wooldrift-farm.html.
  • 7. 50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (7) Cole The dairy industry has been a leader Source: http://vet.tufts.edu/tas/images/002.png. Source: http//www.shopbrownswiss.com/.
  • 8. 50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (8) Cole Feeding the dairy cow Top: Automated system for measuring feed intake. Bottom: Automated feeding system being installed at Embrapa Gado de Leite.
  • 9. 50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (9) Cole Watering the dairy cow Top: Automated waterer with scale for measuring intake. Bottom: Automated scale that weighs cows at the waterer.
  • 10. 50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (10) Cole Monitoring the dairy cow Source: http://support.smaxtec-animalcare.com/. Source:http://c-lockinc.com/. Source:http://www.afimilk.com/.
  • 11. 50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (11) Cole Milking the dairy cow Source: http://www.afimilk.com/. Source: http://goo.gl/wu8YtR. Source: http://www.afimilk.com/. Manufacturers such as Afimilk and DeLaval provide intelligent milk meters, inline milk analysis sensors (e.g., AfiLab), and herd management systems (e.g., Herd Navigator).
  • 12. 50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (12) Cole …and innovation is ongoing Photos courtesy of Albert de Vries. The Swedish Agricultural University dairy research center has a state-of-the-art facility equipped with the latest DeLaval technology.
  • 13. 50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (13) Cole What else comes out of the cow? Environment chambers at Embrapa Gado de Leite, Coronel Pacheco, MG, Brasil.
  • 14. 50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (14) Cole Other uses of on-farm technology Left, middle: Dairies in Germany and Italy sell energy from biogas plants and rooftop solar cells. Right: A dairy in Germany sells fresh milk directly to consumers from an automated, on-farm shop.
  • 15. 50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (15) Cole But you don’t get something for nothing  New technologies often require considerable capital investment.  They sometimes fail to work as advertised, or do not deliver the promised gain.  The data are often most useful when combined with observations from many farms.
  • 16. 50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (16) Cole What challenges are on the horizon?  Monthly milk samples are too infrequent for modern management.  Many large farms do not see a value proposition in milk recording.  The amount of data collected on-farm are growing, but they are not being collected in a central database.
  • 17. 50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (17) Cole Trait Relative emphasis on traits in index (%) NM$ 1994 NM$ 2000 NM$ 2003 NM$ 2006 NM$ 2010 NM$ 2014 GM$ 2014 Milk 6 5 0 0 0 -1 -1 Fat 25 21 22 23 19 22 20 Protein 43 36 33 23 16 20 18 PL 20 14 11 17 22 19 10 SCS –6 –9 –9 –9 –10 –7 -6 UDC … 7 7 6 7 8 8 FLC … 4 4 3 4 3 3 BDC … –4 –3 –4 –6 –5 -4 DPR … … 7 9 11 7 19 HCR … … … … … 2 3 CCR … … … … … 1 5 CA$ … … 4 6 5 5 5 Our focus has changed over time
  • 18. 50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (18) Cole New phenotypes should add information low high Genetic correlation with existing traits lowhigh Phenotypiccorrelation withexistingtraits Novel phenotypes include some new information Novel phenotypes include much new information Novel phenotypes contain some new information Novel phenotypes contain little new information
  • 19. 50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (19) Cole What do current phenotypes look like?  Low-dimensionality  Usually few observations per lactation  Close correspondence of phenotypes with values measured  Easy transmission and storage
  • 20. 50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (20) Cole What do new phenotypes look like?  High dimensionality  Ex.: MIR produces 1,060 points/obs.  Disconnect between phenotype and measurement  More resources needed for transmission, storage, and analysis
  • 21. 50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (21) Cole Name Chrome Location (Mbp) Freq of minor haplotype Gene Name HH1 5 63.15 1.92 APAF1 HH2 1 94.8 to 96.6 1.66 unknown HH3 8 95.41 2.95 SMC2 HH4 1 1.27 0.37 GART HH5 9 92 to 94 2.22 unknown JH1 15 15.70 12.10 CWC15 JH2 26 8.81 to 9.41 1.3 unknown BH1 7 42.8 to 47.0 6.67 unknown BH2 19 10.6 to 11.7 7.78 unknown AH1 17 65.92 13.0 UBE3B Phenotypes may come from genotypes For a complete list, see: http://aipl.arsusda.gov/reference/recessive_haplotypes_ARR-G3.html.
  • 22. 50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (22) Cole Genotypes in the national database 0 100000 200000 300000 400000 500000 600000 700000 800000 NumberofGenotypes Run Date Imputed, Young Imputed, Old <50k, Young, Female <50k, Young, Male <50k, Old, Female <50k, Old, Male 50k, Young, Female 50k, Young, Male 50k, Old, Female 50k, Old, Male
  • 23. 50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (23) Cole Genotyped ancestors, actual bull(HOUSA73431994) 777K 777K 50K - - - 50K 50K 50K 50K 777K 777K 50K - - - 3K 777K 50K 50K 777K 777K 50K Imputed Imputed 50K 50K Imputed 50K 777K 50K 50K 50K 777K 77K - - - 777K 777K 50K Imputed Imputed 50K 50K Imputed 50K 777K 50K 50K 3K 777K 9K 50K 50K 777K 50K 50K 50K 777K 50K Imputed 777K 777K 50K - - - Imputed 777K 50K Genotyped or imputed animals Both parents All 4 grandparents All 8 great grandparents All 16 great, great grandparents 28 of 32 great, great, great grandparents 56 of 60 ancestors in pedigree
  • 24. 50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (24) Cole Genome assembly (simplified) Reads must be assembled into chromosomes Assembly is a computational process (Liu et al., 2009; Zimin et al., 2009) This process is imperfect – repetitive regions are hard to assemble correctly! Sometimes, this… should be this.
  • 25. 50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (25) Cole Possible assembly problem on BTA18 This could be a GC-rich region (bias in Illumina chemistry). More reads than expected may align here because repetitive elements were combined during assembly.
  • 26. 50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (26) Cole Can it be corrected using long reads? • BTA18 genomic DNA extracted from CHORI-240 BAC library (L1 Domino 99375) at AGIL • Sequencing libraries constructed at USDA MARC, pooled, and run on PacBio RS II BAC ID Insert size (bp) Start End CH240-389P14 174,682 56,954,654 57,129,335 CH240-234E12 178,618 57,058,248 57,236,865 CH240-280L6 175,831 57,092,237 57,268,067 CH240-34N7 158,841 57,129,383 57,288,223 Source: Pacific Biosystems
  • 27. 50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (27) Cole Conclusions  Modern sensor technology is routinely producing large amounts of data.  Those data have the potential to improve herd management and profitability.  They can support development of new management practices and research into novel phenotypes.
  • 28. 50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (28) Cole Acknowledgments • AFRI Competitive Grant No 2013-68004- 20365, “Improving Fertility of Dairy Cattle Using Translational Genomics” • Cooperative Dairy DNA Repository • Council on Dairy Cattle Breeding • Paul VanRaden and George Wiggans, AGIL • Albert de Vries, University of Florida • Kent Weigel, University of Wisconsin
  • 29. 50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (29) Cole Note Mention of trade names or commercial products in this presentation is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture.
  • 30. 50th National DHIA Annual Meeting, Columbus, OH, March 10, 2015 (30) Cole Questions?