1. PCR : POLYMERASE CHAIN REACTION

4. INTRODUCTION PCR is one of the most important technique and it is also a key procedure in numerous basic studies involving DNA molecules. All methods for PCR are based on the concept of DNA strand complementarity .

5. A NEW ROLE OF GLYPICAN-3 IN HEPATOCELULAR CARCINOMA

6. A NEW ROLE OF GLYPICAN-3 IN HEPATOCELULAR CARCINOMA GPC3 is a member of the glypican family of glycosylphosphatidylinositol-anchored cell-surface heparan sulfate proteoglycans.

7. A NEW ROLE OF GLYPICAN-3 IN HEPATOCELULAR CARCINOMA GPC3 is highly expressed in HCC cells and tissues. It is thought that GPC3 joins a multiprotein complex, which is composed of the ligand, receptor, GPC3, and probably other proteins.

8. A NEW ROLE OF GLYPICAN-3 IN HEPATOCELULAR CARCINOMA This is the first study to report that GPC3, in conjunction with MMPs and growth signaling molecules, might play an important role in the progression of HCC.

9. OBSERVATION The PCR is an important, useful and fast technique that could help find an appropriate diagnosis before symptom expression.

10. CERTAIN LABORATORY TECHNIQUE ALLOWS RAPID DETECTION OF EYE PATHOGENS

11. The real-time PCR assay delivered results within two hours, whereas culture results were examined after 48 hours. CERTAIN LABORATORY TECHNIQUE ALLOWS RAPID DETECTION OF EYE PATHOGENS

12. Although PCR has a high risk of false positivity, This may demonstrate a better detection sensitivity in the PCR assay. CERTAIN LABORATORY TECHNIQUE ALLOWS RAPID DETECTION OF EYE PATHOGENS

13. CERTAIN LABORATORY TECHNIQUE ALLOWS RAPID DETECTION OF EYE PATHOGENS Real-time PCR can accurately and simultaneously detect bacterial and fungal pathogens in a speedy fashion.

14. OBSERVATION The PCR technique should be a requirement for eye pathogens diagnosis, because it leads to a diagnosis and therefore more specific treatments.

15. MEDICAL UTILITY

16. MEDICAL UTILITY Polymorphism diagnostic Characterization for transplants Establish mutation

17. DNA clonation Mutation diagnostic Detection of tumor cells MEDICAL UTILITY

18. Prenatal diagnosis disease Genetic screening Detection of infectious microorganisms MEDICAL UTILITY

19. BIBLIOGRAPHY MARTINEZ SANCHEZ,Lina María, Biología Molecular, 5 edición, Medellín, UPB, Fac de Medicina 2009, 139p-147p. http://www.sciencedaily.com/releases/2010/05/100510161300.htm http://www.sciencedaily.com/releases/2010/08/100802091207.htm

20. PICTURE Mejor es callar y que sospechen de tu poca sabiduría que hablar y eliminar cualquier duda sobre ello. Abraham Lincon