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Isoenzymes

  1. 1. ISOENZYMES -Keshava Pavan K
  2. 2. • Multiple forms of same enzyme catalysing the same reaction • Differ in physico-chemical properties
  3. 3. • Amino acid composition – Electrophoretic separation • Heat stability – Placental and intestinal isoenzymes of the 6 isoenzymes of alkaline phosphatase are heat stable • Susceptibility to inhibitors – Acid phosphatase-6 isoenzymes – Prostate portion is tartarate labile – Bone portion is urea labile
  4. 4. • Enzyme kinetics – Km and Vmax differ • Immunological properties – Eg,. CKMB estimation • Different coenzymes – Isocitrate dehydrogenase – Mitochondria- NADH – Cytoplasm- NADPH
  5. 5. • Synthesized from different genes – Eg,. Salivary and pancreatic amylase are each formed from different genes • Oligomeric enzymes – Lactate dehydrogenase ( polypeptide chains) – Creatine kinase (4 polypeptide chains) • Glycoproteins : different amount of carbohydrate – Eg,. Alkaline phosphatase – sialic acid ( N Acetyl neuraminic acid)
  6. 6. • Produced from same locus of gene in different persons – G6P dehydrogenase has 400 types.

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