useful for BSC nursing and BPT 1st year students. Covers the topics as per syllabus recommended by baba farid university of health sciences.

1. Proteins Shiv Ram krishn Senior Demonstrator, Biochemistry, GSMCH.

6. How amino acids combine to form protein

12. Thanks

13. Classification of Proteins Shape & Size Functional Properties Solubility & Physical Properties Fibrous Globular Defence proteins Contractile Proteins Hormones Enzymes Structural proteins Respiratory Proteins Simple Proteins Conjugated Proteins Derived Proteins Protamines Scleroproteins Glutelins Gliadins (Prolamines) Globulins Albumins Histones Nucleo proteins Metallo proteins Lipoproteins Phospho proteins Chromo proteins Glycoproteins Muco Proteins Primary derived Secondary derived Proteans Coagulated proteins Metaproteins Proteoses peptides Peptones

14. Simple Proteins Basic Protein, N.A. + Histone=Nucleoprotein, Porphyrin+Histone=conjugated Protein, Repressor of template activity of DNA in synthesis of RNA’ Hemoglobin  Globin  Histone  His & lys rich Alkaline Arginine, Histidine _ NH3 Water, Dil. Acid, Salt solution Histones Salmine Sardine Cyprinine(of fish sperms & testes) Basic protein, N.A+protamine=Nucleoprotein 7.4 Cysteine Tryptophan tyrosine Arginine rich _ Water Dilute acid Dilute alkali Dilute NH3 small Protamine Eg. Use pI AA- AA+ Heat coagulability Insolubility Solubility Size & shape Protein type

15. Simple proteins contd. Rice  Oryzein, Wheat  Glutelin Glutamic acid rich + Water, Neutral salt solution Dilute acid, Dilute alkali Large molecules Glutelins Wheat-  Gliadin, Barley  Hordein Plant Protein Lysine Proline Water, Salt soln., Absolute alcohol Alcohol(50-80% C2H5OH) Gliadin (Prolamines) Heme+globulin=Hemopexin, Heme+metal=Transferrin, Ceruloplasmin, Heme+Carbohydrate=Immunoglobulin, Eggs  Ovoglobulin Milk  Lactoglobulin Legumes  Legumin Precipitated by half saturation with ammonium sulfate or full saturation with sodium chloride. + water Dilute neutral salt solutions Globulins Legumes—legumelin, Cereals—Leucosin, Egg—Ovalbumin Milk--Lactalbumin Precipitated out of solution by saturation by ammonium sulfate. 4.7 Glycine Cagulable by heat  Product  insoluble in water & dil. Salt solution Water, Dilute salt solution Albumin

16. Simple proteins contd. scleroproteins Found in Connective tissue, In Yellow elastic fibres, In ligament, tendon Formed in large amount during pregnancy Elastin is hydrolyzed by pancreatic elastase Cysteine, Methionine, 5-Hydroxylysine, Histidine Alanine, Leucine, Valine, Proline Elastins Found in connective tissue,Bone Collagen  Tannic acid  tough, hard, Collagen  boiling  gelatin  highly soluble, easily digestible  cool  gel  no tryptophan Insoluble in all neutral salt solvents Long, thin, partially crystalline Collagen Present in chidermal tissuesuch as horn, hair, nails, wool, hoofs, feathers(supporting structures) spider’s web, silk, reptilian scales Cysteine absent Hard keratin  His:Lys:Arg  1:4:12, Soft or pseudokeratins  A.A. not in same ratio, Neurokeratins  1:2:2, Human Hair  alpha keratin  cysteine rich, Beta Keratin  Glycine, Alanine rich low Fibrous Keratins