1. Biotechnology Review
QUIZ FEBRUARY 12TH

2. “ Ashley donated a regular body cell and Jesse donated
an egg cell. Jesse’s egg cell had its nucleus removed.
Ashley’s cell nucleus was transferred from the body cell
into the egg cell. Cell division occurred and the new
embryo was implanted into Madison, the surrogate
mother. Madison eventually gave birth to the baby.
Who does the baby resemble? ”
Ashley

3. Who committed
the crime?
Suspect #2

4. Goats that make spider silk is an example
of…
a. Genetic Engineering
b. Stem Cell Therapy
c. DNA fingerprinting
d. PCR
A. Genetic Engineering

5. What might we be able to treat
Parkinson’s Disease with?
A. Genetic Engineering
B. Stem Cells
C. Cloning
D. Recombinant DNA
B. Stem Cells

6. What is a genome?
All of the genes within an organism

7. What is the Human Genome
Project?
Mapping of all of the genes within humans

8. Why does DNA move through the
gel during gel electrophoresis?
Negative charge, moves towards positive
electrode

9. Who is this?
Dolly

10. Making an identical copy of an
organism is known as what?
Cloning

11. What is so great about a stem
cell?
Function has not yet been determined, can
differentiate into different cell types

12. What do we use to cut DNA?
Restriction Enzymes

13. What organisms are restriction
enzymes naturally found in?
Bacteria

14. What purpose do restriction
enzymes serve in bacteria?
Protection from viruses

15. If a restriction enzyme cuts at the
sequence CC/GG what kind of
ends would we end up with on the
DNA fragments?
Blunt Ends

16. EcoRI recognizes the following sequence: G A A T T C
CTTAAG
What types of ends would this cut result in?
Sticky

17. The longest
fragment is in
which lane?
Lane D

18. The shortest
fragment is in
which lane?
Lane A

19. What is the purpose of a DNA size
standard?
Gives known fragment lengths of
DNA

20. The left column is the
DNA Standard of
100, 50, and 10.
What would the
band lengths be in
the second column?
About 75 and 50

21. What is a plasmid?
Circular DNA in bacteria

22. EcoR1 recognizes the sequence
GAATTC. How many fragments
would this piece of DNA be cut
into?
2 cuts, so 3 fragments

23. What are the basic steps in
cloning?
Place nucleus of somatic cell into egg cell that
has it’s nucleus removed, place into surrogate

24. What is the difference between an
egg cell and a somatic cell?
Somatic cell has full set of
chromosomes, egg cell has a half set
of chromosomes