1.
MicologiaForestal&Aplicada.www.micofora.com
Tuber melanosporum mycelium concentration varies with
bacteria and organic compounds treatments
@xvilanovasola IRTA –Micofora research project 2017
xaviervilanova@micofora.com

2.
MicologiaForestal&Aplicada.www.micofora.com
New data on host tree and truffle nutrition and
interacions with rhizosfere
Primeres tòfones 3,5 anys Primeres tòfones13 anys
>1000mm pluja + reg
Sòl franc, ric i orgànic
450mm pluja sense reg
Sòl argilós i pobre

3.
MicologiaForestal&Aplicada.www.micofora.com
We tested 8 bacteria strains + 6 organic compounds on
nursery and plantations
ê
í î

4.
MicologiaForestal&Aplicada.www.micofora.com
Tuber melanosporum mycelium concentration varies with
bacteria and organic compounds treatments
EXPERIMENT DESIGN
8 bacterial treatmanet (TR1-TR9), 6 organic compounds (TR10-TR15) and 1
control (TR4).
We established 15 lots of 10 trees each (60 trees/lot for nursery trials) in 3
places: IRTA nursery, 3,5 years old plantation in Batea (Tarragona) & 10 years
old plantation in Barcelona.
3 months later we quantified qith Real Time PCR the concentration of black
truffle mycelium in each treatment.
We´ll corralate as well concentration of truffle mycelium with:
• % mycorrhization level
• Tree trunk diameter
• Host tree nutritional fitness (foliar analysis)
• Truffle yields

5.
MicologiaForestal&Aplicada.www.micofora.com
Tuber melanosporum mycelium concentration varies with
bacteria and organic compounds treatments
0
5
10
15
20
25
30
TR1 TR2 TR3 TR4 TR5 TR6 TR7 TR8 TR9
TR10TR11TR12TR13TR14TR15
Serie1
NURSERY
0,00E+00
2,00E+07
4,00E+07
6,00E+07
v1
v2
v3
v4
v5
v6
v7
v8
v9
v10
v11
v12
v13
Serie1
1,00
1000,00
v1 v3 v5 v7 v9 v11 v13
Millares
Serie1
Values in mg of mycelium/gram of soil

6.
MicologiaForestal&Aplicada.www.micofora.com
Tuber melanosporum mycelium concentration varies with
bacteria and organic compounds treatments
0
0,2
0,4
0,6
0,8
1
1,2
1,4
1,6
TR1
TR2
TR3
TR4
TR5
TR6
TR7
TR8
TR9
TR10
TR11
TR12
TR13
TR14
TR15
Serie1
BATEA (3,5 years old)
0,0
20000,0
40000,0
60000,0
b1
b2
b3
b4
b5
b6
b7
b8
b9
b10
Millares
Serie1
0,0
1000,0
2000,0
3000,0
4000,0
5000,0
6000,0
b1
b2
b3
b4
b5
b6
b7
b8
b9
b10
Millares
Serie1
Values in mg of mycelium/gram of soil

7.
MicologiaForestal&Aplicada.www.micofora.com
Tuber melanosporum mycelium concentration varies with
bacteria and organic compounds treatments
0
2
4
6
8
10
12
Serie1
Granollers (10 years old)
0,0
10000,0
20000,0
30000,0
40000,0
50000,0
G1
G2
G3
G4
G5
G6
G7
G8
G9
G10
Millares
Serie1
0,0
500,0
1000,0
1500,0
g1 g2 g3 g4 g5 g6 g7 g8 g9 g10Millares
Serie1
Values in mg of mycelium/gram of soil

8.
MicologiaForestal&Aplicada.www.micofora.com
Tuber melanosporum mycelium concentration varies with
bacteria and organic compounds treatments
CONCLUSIONS
• Each stage (age) of the plant has a different reaction for each treatment.
• In nursery phase, 11 of the 14 treatments generated a positive effect on the
concentration of mycelium. Mainly two organic substances (TR11 and
TR12) and a bacterial strain (TR9).
• In plantation 3.5 years: all treatments generated a positive effect on the
concentration of truffle mycelium in the soil. Mainly two organic substances
(TR14 and TR15) and two bacterial strains (I TR9 TR6).
• In 10 years plantation 5 to 14 treatments generated a positive effect on the
concentration of truffle mycelium in the soil, all organic substances
(TR10,11,13,14 I15).

9.
MicologiaForestal&Aplicada.www.micofora.com
Tuber melanosporum mycelium concentration varies with
bacteria and organic compounds treatments
@xvilanovasola IRTA Barcelona 17th january 2017
xaviervilanova@micofora.com
What comes next:
To choose best treatment for each
host tree age and combine the best
treatments together