Ribotyping is a molecular technique for bacterial identification that uses information from rRNA-based phylogenetic analyses. It is rapid and specific method widely used in clinical diagnostics and analysis of microbial communities in food, water, and beverages.

3. summary
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4. What you can expect to know:
0 Introduction
0 Different techniques of ribotyping
0 Adventages and limitations
0 Literature review
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5. definition
Why rdna? it means…..
1.Universally present 2.large and stable 3.high
degree sequence conservation classification
Ribosomal RNA r DNA description-identification-nomenclature
ribotyping
Ribo … …typing
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7. Organization of ribosomal operon
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9. Conventional ribotyping
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11. Selection of restriction endonuclease by sequence analysis(in silico)
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Ribotyping prototype : Haemophilus influenzae strain Rd
In silico analysis
The presence of 6 ribosomal operon

12. Ribotyping protocol for Haemophilus
influenzae strain Rd
Stage 1-search the conserved restriction endonuclease site within the six
ribosomal operon.
Stage 2-choose the ideal restriction enzyme
Stage3-confirm the conservation of restriction site by the all publicity
available for 16-23s-rRNA sequencing
Stage4-cleavage electrophoresis southern blot hybridization with
a labelled probe containing conserved ribosomal sequences.
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13. Cont.
0 The importance of this method :strain differentiation within the species
0 It implemented for several other bacterial species,eg : S.aureus
E.coli
p.aeroginosa
B.cepacia
N.meningitidis
B.pertussis
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14. Automated ribotyping
0 Was first introduced by dupont qualicon-1995….. Riboprint pattern.
0 This system is reproducible ,convenient and fast
0 A single colony of bacteria is picked up and suspended in lysing buffer
and transferred to the riboprinter in the riboprinter like
conventional ribotyping….
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15. PCR Ribotyping
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16. Advantages and disadvantages
Advantages of ribotyping as an identification method:
0 Ribotyping is a fully automated procedure
0 Procedure involves less labor and is standardized
Disadvantages of ribotyping:
0 Expensive because of the equipment used, therefore usually
only performed in reference laboratories

17. Literature review

22. references
• Grimont, F., and P. A. D. Grimont. 1986. Ribosomal ribonucleic acid
gene restriction patterns aspotential taxonomic
tools. Ann. Inst. Pasteur Micro- biol.
• Altwegg, M., F. W. Hickman-Brenner, and J. J. Farmer III. 1989.
Ribosomal RNA gene restriction patterns provide increased sensitivity
for typing Salmonella typhi strains. J. Infect. Dis. 160:145–149.
• Bingen, E. H., P. Mariani-Kurkdjian, N. Y. Lambert-Zechovsky, P.
Dejardins, E. Denamur, Y. Aujard, E. Vilmer, and J. Elion. 1992.
Ribotyping provides efficient differentiation of nosocomial Serratia
marcescens isolates in a pediatric hospital. J. Clin. Microbiol.
30:2088–2091.

23. Cont.
Farmer, J. J., III. 1972. Epidemiological differentiation of Serratia marcescens:
typing by bacteriocin sensitivity. Appl. Microbiol. 23:226–231.
• Animal Biotechnology: Models in Discovery and Translationedited by
Ashish Verma, Anchal Singh

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