I'Screen STREPTO is Tecna ELISA test kit for the rapid, reliable and sensitive detection of streptomycin and dihydrostreptomycin in honey. Its easy sample preparation enhables end-users to achieve a high sensitivity (5 ppb) with a solvent-safe approach. Good correlation to LC-MS analysis were obtained. Visit our web site www.tecnalab.com

1. Enzyme immunoassay for the detection of STRETOMYCIN in honey
Code AB650

2. Regulatory aspects at a glance
UK: 20 ppb
Belgium: 20 ppb
France: 10 ppb Switzerland: 20 ppb
US: no limits fixed by FDA Italy: 10 ppb (CCβ for screening)
(CCβ
5 ppb (action limit - confirmatory methods)
India: 10 ppb
Australia:
Not allowed
ppb = ng/g or µg/kg

3. Features
Assay Principle
Competitive enzyme immunoassay Standard curve example
Applications
HONEY
Calibration range
0.1 - 20 ng/ml
Detection limit
Honey: 2 ppb
Assay time
60 minutes
Specificity
Streptomycin 100%
Dihydrostreptomycin >100%

4. Honey preparation procedure
Dilution, filtration
[1 g of honey + 19 ml dilution buffer 1x;
shake until homogeneity; filter with Whatman 1]
Or
[1 g of honey + 9 ml diluition buffer 1x;
shake until homogeneity; filter with Whatman 1; dilute 1+1]

5. Assay validation
SPECIFICITY AND SENSITIVITY
20 blank raw honey samples were analysed.
The mean B/Bo was 96±7%.
The Limit of Decision was set at 82% relative signal
(corresponding to 3.8 ppb)
The specificity was 95%
The CCβ was set at 5 ppb of streptomycin, with
97% sensitivity.
Validation according
to 657/2002 (EC)
The mean B/Bo of spiked honey was 75±7%.
The mean difference to blanks was 21% B/Bo.

6. Assay validation
ACCURACY
30 streptomycin incurred honey samples
were provided from different laboratories
and analysed in parallel by I’Screen
Strepto and LC/MS.
The mean recovery was 112±28%
PRECISION
Intra-assay CV 11%
Correlation between LC/MS and I’Screen Strepto results
Inter-assay CV 18%

7. Advantages
Easy, fast and versatile sample preparation
Easy assay implementation
Add standards / samples and the conjugate
Incubate 30 minutes at ROOM TEMPERATURE
Wash 3 times
Add the developing solution
Incubate 30 minutes at ROOM TEMPERATURE
Add the stop solution and read
Sensitivity in compliance to all requirements
Good correlation with confirmatory methods

8. More
HONEY SCREENING
Tecna’s portfolio of ELISA test kits for rapid
and sensitive detection of veterinary drug
residues in honey
SuperScreen Tetra HS - screening four tetracyclines, oxytetracycline included
I’Screen SULFA and I’Screen SULFA QL - screening fifteen main sulfonamides in
honey, three sample preparations validated
I’Screen AOZ and I’Screen AMOZ v2 - screening furazolidone and
furaltadone metabolites respectively
I’Screen CAP - screening chloramphenicol at 0.15 ppb in honey
Quinolones v2 ELISA KIT - screening six main
quinolones in honey
I’Screen Tylosin - screening tylosin A in honey

9. Instruments and services
More
Aquaculture
Other foods
Allergens
Honey
Cereals
Diary
Water
Meat
Feed
TECNA
CATALOGUE
Find what best fits your
screening necessities in
different fields, for different
analytes.
Contact our team of experts for
technical support
Require our Performance Data
Sheets to see our in-house
validative results
Download our spreadsheet for
a fast and easy result
calculation
Contacts
export@tecnalab.com
servizioclienti@tecnalab.com