In this talk, Prof. Mohamed L. Salem presents the importance of having a center of excellence at each institute to enhance and foster scientific research and innovation.

1. Cairo University, Egypt
15 October, 2016
Trans-Disciplinary Research Team
Is A Must for Excellence In Science
“Tanta University as a success story”
Mohamed Labib Salem, PhD
Prof. of Immunology
Faculty of Science, Tanta University, Egypt

2. The Talk agenda
1. Research Needs
2. Research Team Works
3. Research Capacity
4. Success Stories

3. Microbe
Immune
Interaction
Cancer
Immune
Interaction
HCV
Immune
Interaction
Cancer
Biology
CTC/CSC

4. 1894 -1990
1992 – 1995
1995-1997
2010-2014
2001-2010
1
2
1997 - 2001
4 3
5
6

5. Research Needs

6. Research Elements
Experience
Expertise
Infrastructure
Skills
Knowledge

7. Research Needs
Communication
Lab Space
Budget
Manpower
Leadership

8. Science
Patents
Publications
Competitiveness Internationalization
Marketing
Research Outputs

10. Types of Research Teams

11. Types of Research Teams
A
•Multidisciplinary
B
•Interdisciplinary
C
•Transidsciplinary

12. Multidisciplinary Research Team
Multidisciplinarity draws on
knowledge from different
disciplines but stays within
their boundaries.

13. Interdisciplinarity analyzes,
synthesizes and harmonizes links
between disciplines into a
coordinated and coherent whole.
Intertidisciplinary Research Team

14. Transdisciplinarity integrates the
natural, social and health sciences
in a humanities context, and
transcends their traditional
boundaries.
Transtidisciplinary Research Team

18. Sociogram
of
a
Transdisciplinary
Team
Betweenness Centrality

19. Research Team
Leadership

20. Management versus Leadership

23. Transdisciplinary leaderships
1- Effective cognitive leadership:
Provides a vision that links and
motivates transdisciplinary
researchers to step beyond their
disciplinary lens, relax old
assumptions, and search for
creative frame-breaking solutions.

24. Transdisciplinary leaderships
2- Effective structural leadership:
Adds value by creating
needed bridges among
unconnected parties.

25. Transdisciplinary leaderships
3- Effective processual leadership:
Encourages trust and turns
potentially destructive conflict
into constructive interactions.

26. Research Team Phases
(Research Capacity)

27. Levels of Research Capacity
Building

28. Levels of Research Capacity
Building

29. 5 Elements of an Organization’s Design
The
Star
Model
Strategy
People Structure
Rewards Process

30. Research Capacity
Development

31. Phases of Research Capacity Development

32. Tanta University
1

34. Grants, Innovation & Technology Transfer Center
• Technology Transfer Office (TTO)
• Grants and International
Cooperation Office (GICO)
• Technology Innovation Support
Center (TISC)

37. National and International
funded projects
(2010-2016)
> 45 Millions L.E.

38. • Tanta University Central Lab (5m LE; STDF)
• Tanta University CECR (10m LE; STDF)
• Faculty of Medicine (7m; 3 projects; STDF)
• Faculty of Science (4 projects; 6m; STDF)
• Faculty of Agriculture (1 project; Mobility, FP7)
• Faculty of Engineering (5 projects, 10m; STDF)
• Ministry of Int Cooperation (about 1m)
• Academy of Scientific Research & Technology (2m)
• Ministry of Higher Education (about 10m)
Medicine, Engineering, Science, Education
National and International Grants

39. Center of Excellence
in Cancer Research (CECR)
2

40. STDF Grants for Centers of Scientific Excellence (STDF-CSE)
‫السرطان‬ ‫ألبحاث‬ ‫التميز‬ ‫مركز‬
CENTER OF EXCELLENCE IN
CANCER RESEARCH (CECR)

41. Center of Excellence in Cancer Research,
Tanta University
(CECR)
‫طنطا‬ ‫بجامعة‬ ‫السرطان‬ ‫ألبحاث‬ ‫التميز‬ ‫مركز‬

42. Established Date
 June 2013 through a project funded by Science and
Technology development Fund (STDF)
 PI: Prof. Mohamed Labib Salem
 Affiliation: Tanta University
 Budget: 9,657,500 L.E
 Duration: 36 Months
Established Date

43. CECR From Application to Funding
STDF-CSE Call
Proposals
Evaluation
Interview & Oral
Presentation
Site Visit
146 Project
61 Project
25 Project

47. Location
Tanta University Educational Hospital
Location

48. ‫الفري‬
‫ق‬

49. ‫بالقاهرة‬ ‫العقد‬ ‫توقيع‬ ‫أثناء‬ ‫الفريق‬

50. ‫بالقاهرة‬ ‫العقد‬ ‫توقيع‬ ‫أثناء‬ ‫الفريق‬

51. ‫طنطا‬ ‫جامعة‬ ‫في‬ ‫العقد‬ ‫توقيع‬ ‫أثناء‬ ‫الفريق‬

52. ‫طنطا‬ ‫جامعة‬ ‫في‬ ‫العقد‬ ‫توقيع‬ ‫أثناء‬ ‫الفريق‬

53. Prof. Mohamed L.Salem
Director, CECR
Prof. Said Hammad
Vice Director, CECR
Prof. Mohamed Ramadan
Clinical Director, CECR
Prof. Mohamed Attia
Director of Flow Cytometry Unit, CECR
Dr. Abdel-Aziz Zidan
Director of Proteomic & Genomic Unit, CECR
Ms. Mona F. Zidan
Research Assistant
Research Team
Eng. Heba M. Wassfy
Coordinator , CECR
Dr. Enas Abdul-Raouf
Research Assistant

54. Organogram

55. CECR Core Facilities

56. CECR Vision
Create omic based core facilities
to lead cutting edge translational
cancer research

57. CECR Mission
 Create a rich academic environment for
excellence in cancer research to lead the next
generation of scientists and empower the
discovery and entrepreneurships in cancer
prognosis, diagnosis, prevention, and treatment
through efficient collaboration with national and
international esteemed investigators.

58. 1. Establish the CECR to perform cutting-edge
preclinical and clinical research.
2. Correlate immunologic and clinical
performance between genomic,
transcriptomic and proteomic profiling of
circulating tumor cells
Two CECR Main Aims

59. CECR Goals
CECR
GOALS
Training
Outreach
Office
Research
from bench to
clinic
Independent
Researchers
Technical/
Clinical
Consultation
Grants
Publications
Patents
Scientific
Collaboration

60. Work on
personalized
medicine
Establish
Infrastructure
Publication
Sustainability
Training and
Outreach
Phase 1
Phase 2
CECR
Activities

61. CECR Core Facilities

62. Immuno-Phenotyping Core Facility

63. Immuno-Phenotyping Core Facility
 Flow Cytometry Unit
 Cell Sorting Unit

64. Immuno-Phenotyping Core Facility
 Flow Cytometry Unit
 Cell Sorting Unit

65. Nomination:
The BD FACSCanto derives its name from the musical term “bel canto” meaning
operatic singing that focuses on ease and purity of tone with an agile and precise
vocal technique.
Flow Cytometry Unit
• BD FACSCanto II Cytometer

66. •
The BD FACSCanto system has a capacity for three lasers, eight-parameter
detection while capturing particle sizes from 0.5 to 50 μm in diameter.
•
Digital electronics for processing up to 10,000 events per second, and a
novel sample injection tube supporting
Multiparameter detection helps in:
•
Advanced cell subset analysis.
•
Multiparameter functional studies.
•
Soluble protein detection.
•
Quantification studies for cell-surface antigens and soluble proteins.
•
Decreases the total sample volume necessary for a specific project as more
parameters.
Specifications of the instrument
Specifications of the instrument

67. Flow Cytometry Unit
 FDA Approved
 Research & Diagnosis

68. Stem cell
enumeration
Detection of minimal
residual disease
BD FACSCanto Applications
Analysis of
Leukemias and
Lymphomas
Reticulocyte
analysis
Detection of
Immunodeficiency
diseases
Detection of
HIV infection
Analysis of
different types of
solid tumors.
Detection of
autoantibodies
Research & Clinical applications of the instrument
BD FACSCanto Applications

69. Immuno-Phenotyping Core Facility
 Flow Cytometry Unit
 Cell Sorting Unit

70.  The IsoFlux system enriches rare cells from biological samples and
prepare them for further analysis , It gives immediate access to high-
quality , viable cells in a high density format , ready for use in the
application of your choice
Cell Sorting Unit

71. Cell Sorting Unit
IsoFlux Applications
Enumeration Immunofluorescence
Fluorescence in
situ hybridization
Next generation
sequencing
Mutation
detection
Gene
expression

72. Omic Core Facility

73. Omic Core Facility
 Genomic Unit
 Proteomic Unit

74. Omic Core Facility
 Genomic Unit
 Proteomic Unit

75. Microarray
A high throughput technology that allows detection of thousands
of genes simultaneously.
Genomic Unit

76. Genomic Unit
Microarray Applications
Transciptome
mRNA Expression
miRNA Expression
Splice Variance
Genome
Structure
DNA Copy number
Genome Variation
Single Nucleotide
Polymorphism
Copy number Variations
Proteome
Protein-Protein Interactions
DNA – Protein intreraction
Epigenetics
DNA Methylation
Chromatin Structure

77. Agilent 2100 Bioanlayzer.
It is the industry standard for RNA
sample QC and has replaced labor-
intensive gel electrophoresis for
this application, It is also rapidly
replacing gel electrophoresis for
DNA fragment analysis and SDS-
PAGE analysis of protein samples.
Genomic Unit

78. Genomic Unit
Bioanalyzer Applications
Whole Cells
RNA
RNA Integrity
RNA Qualification
Small RNA Sizing
DNA
DNA Integrity
DNA Qualification
Protein
Protein Integrity
Protein Qualification
Protein Purity

79. Gel Documentation System
(Biometra Doc Analyze)
Enables simple documentation of
fluorescent and non-fluorescent gels,
membranes, blots, and assays with the
ability to save images to disk for later
quantitative analysis or enhancement for
publication.
Genomic Unit

80. Gene AmpPCR System 9700
It is an automated instrument,
specifically designed for the
amplification of nucleic acids using the
Polymerase Chain Reaction (PCR)
process
Genomic Unit

81. Omic Core Facility
 Genomic Unit
 Proteomic Unit

82. • LumineX200
Proteomic Unit

83. Proteomic Unit
Luminex Applications
Clinical
Diagnostics
Clinical
Research
Life Science
Research
Biosurveillance
Assay Development
Tools

84. Cell Culture Core Facility

85.  Biosafety Cabinet
Cell Culture Unit
 Co2-Incubator  Centrifuge

86. CECR Infrastructure

87. CECR Infrastructure

88. CECR Infrastructure

89. CECR Infrastructure

90. CECR Infrastructure

91. CECR Infrastructure

92. CECR Infrastructure

93. CECR Infrastructure

94. ‫طنطا‬ ‫بجامعة‬ ‫العالمي‬ ‫التعليمى‬ ‫المستشفى‬ ‫افتتح‬ ‫العالى‬ ‫التعليم‬ ‫وزير‬
‫عظيم‬ ‫طبى‬ ‫صرح‬
..
‫وأفريقيا‬ ً‫ا‬‫عربي‬ ‫تسويقه‬ ‫ويجب‬
•
‫إلى‬ ‫تصل‬ ‫مساحة‬ ‫على‬ ‫اقيمت‬ ‫التي‬ ‫المستشفى‬
4,5
‫قاربت‬ ‫بتكلفة‬ ‫فدان‬
2,5
‫للطائرات‬ ‫مهبط‬ ‫وبها‬ ، ‫جنيه‬ ‫مليار‬
.
‫وتضم‬
‫بسعة‬ ‫ونادرة‬ ‫دقيقة‬ ‫طبية‬ ‫تخصصات‬
1000
‫لتخدم‬ ‫سرير‬
20
‫الشيخ‬ ‫وكفر‬ ‫الغربية‬ ‫هى‬ ‫محافظات‬ ‫ثالث‬ ‫فى‬ ‫مواطن‬ ‫مليون‬
‫األوسط‬ ‫الشرق‬ ‫فى‬ ‫مستشفى‬ ‫أكبر‬ ‫يجلعها‬ ‫بما‬ ‫والبحيرة‬
.
‫ف‬ ‫لها‬ ‫مثيل‬ ‫ال‬ ‫التي‬ ‫الحديثة‬ ‫األجهزة‬ ‫من‬ ‫العديد‬ ‫المستشفى‬ ‫وتضم‬
‫ي‬
‫األشعة‬ ‫أجهزة‬ ‫منها‬ ‫مصرية‬ ‫جامعات‬
PET-CT
‫و‬
MULTISLICE320

95. CECR Scientific Activities

98. Circulating Tumor Cells (CTC)

99. CSC
CTC
CTC cause tumor relapse

101. CECR Collaborators

102. CECR Collaborators CECR Collaborators

103. CECR Outreach Activities

105. The 1st Annual Conference for the Middle-
Eastern Association of Cancer Research
27-28/12/2011

107. Monday 24 February 2014
The 1st Annual Conference of
The Center of Excellence in Cancer Research
Next Generation Cancer Research & Diagnosis
In Collaboration with
Research Centers of Excellence
(STDF- Affiliated Centers)
CECR Activities

108. CECR Activities

109. CECR Activities

RNA Extraction Training To CECR Research Team, CECR, Tanta University
Educational Hospital

110. CECR Activities

RNA Extraction Training To CECR Research Team, CECR, Tanta University
Educational Hospital

111. CECR Activities

Prof. Mohamed L. Salem’s visit to children Research Institute, Medical
University of South Carolina, USA

112. CECR Activities

Prof. Mohamed L. Salem’s visit to Medial College of Georgia , Georgia
Regents University, USA

113. CECR Activities

Prof. Mohamed L. Salem’s visit to Medial College of Georgia , Georgia
Regents University, USA

114. CECR Activities

Prof. Mohamed L. Salem’s & Dr. Sohaila Galal’s visit to International 2013
Forum of Immunology. Montreal, Canada

115. CECR Activities

Prof. Gemy Gessy , Prof. of Toxicology, University of Sakatshowan, Canada

Prof. Mohamed Mona, Prof. of Invertebrate Biology, Tanta University

Prof. Elsayed Salem, Prof. of Genetics and Cell Biology, Tanta University

116. CECR Activities: Visitors

Dr Magdy Elhefbawy, President of Gharbia Medical Sector Syndicate

Prof. Gamal Ramadan, Prof. of Immunology, Ain Shams University

117. CECR Activities

Prof. Mohamed Attia’s Training on FACSCanto Diva7 at BD
Biosciences ,Heidelberg-Germany

118. CECR Activities

Dr. Abdel-Aziz Zidan Training Course on “ Affymetrix Geneship System 3000
7G , Stem Cell Center-Jordan University.

119. CECR Activities

Dr. Abdel-Aziz Zidan Training Course on “ Affymetrix Geneship System 3000
7G , Stem Cell Center-Jordan University.

120. CECR Activities

Mona Zidan’s Training course at International Center for Genetic Engineering
& Biotechnology ICGEB “ Flow Cytometry & Cell Sorting in Biotechnology &
Biomedicine Research” Institut Pasteur de Montevideo, Montevideo, Uruguay

121. CECR Activities

CECR Team Workshop on “The first workshop on Application of bioinformatics
in Molecular Diagnostic Virology” . National Cancer Institute, Cairo University

122. CECR Activities

Dr. Sohaila Galal’s Workshop on “ Gene Expression on Large Scale Using
Microarray and Real time PCR Techniques”. Cairo University Research Park

123. CECR Activities

Flow cytometry workshop: Preclinical and Clinical applications

A 3-day workshop

124. CECR Activities

Flow cytometry workshop: Preclinical and Clinical applications

A 3-day workshop

125. CECR Activities

126. CECR Activities

127. CECR Publications

128. CECR Publications
 The Use of Dendritic Cells for Peptide-Based Vaccination in Cancer
Immunotherapy. Mohamed L. Salem
 Immunostimulatory Effects of Triggering TLR3 Signaling Pathway
Implication for Cancer Immunotherapy. Mohamed L. Salem, Said M.
Hammad, Mohamed R. Elshanshory, Mohamed A. Attia, Abdel-Aziz A. Zidan.
 G-CSF/anti-G-CSF antibody complexes drive the potent recovery
and expansion of CD11b+Gr-1+ myeloid cells without
compromising CD8+ T cell immune responses. Mark P Rubinstein,
Mohamed L Salem, Andrew L Doedens, Caitlin J Moore, cody Chiuzan, Guillermo L
Rivell, David J Cole and Ananda W Goldrath

129. CECR Publications
 Understanding the biology of ex vivo-expanded CD8 T cells for
adoptive cell therapy: role of CD62L Immunol Res. Díaz-Montero CM,
Zidan AA, Pallin MF, Anagnostopoulos V, Mohamed L Salem, Wieder E, Komanduri
K, Montero AJ, Lichtenheld MG
 Immunobiology and signaling pathways of cancer stem cells:
implication for cancer therapy. Salem ML, El-Badawy AS, Li Z
 Autologous transplantation of CD34+ bone marrow derived
mononuclear cells in management of non-reconstructable critical
lower limb ischemia. Ismail AM1, Abdou SM, Aty HA, Kamhawy AH, Elhinedy
M, Elwageh M, Taha A, Ezzat A, Salem HA, Youssif S, Salem ML

130. CECR Publications
 IL-12 enhances the function and anti-tumor activity in murine and
human CD8+ T cells. Mark P. Rubinstein, Ee Wern Su, Samantha Suriano,
Colleen A. Cloud, Kristina Andrijauskaite, Pravin Kesarwani, Kristina M. Schwartz,
Katelyn Williams, C. Bryce Johnson, Mingli Li, Gina M. Scurti, Mohamed L. Salem,
Chrystal M. Paulos, Shikhar Mehrotra, Elizabeth Garrett-Mayer, Michael I.
Nishimura, and David J

131. CECR Students

132. CECR Students
9 PhD Students
 15 MSc Students
Adaptive Cell Therapy
Cancer Biology
Anti Cancer effects of medicinal Plans
Stem Call based therapy
Cancer Stem cells
Immunoregulatory cells

133. Tanta University
3

134. ‫انشاء‬
‫لتطو‬ ‫كأداة‬ ‫الجينية‬ ‫البصمة‬ ‫تكنولوجيا‬ ‫مركز‬
‫ير‬
‫السرطان‬ ‫لتشخيص‬ ‫المتقدمة‬ ‫التطبيقية‬ ‫األبحاث‬
Genetic Signature center for fostering next
generation translational
‫الطب‬ ‫كلية‬
‫طنطا‬ ‫جامعة‬

136. microRNAs: tools for cancer
diagnosis

137. MiSeq – NGS
52.3 cm

138. Tanta University
4

139. Immunology and
Molecular Biology Unit
Zoology Department
Faculty of Science
Tanta University

141. Flow cytometer - CyFlow® Cube 6

142. Tanta University
5

144. ‫ا‬
‫رسطان‬‫ل‬‫ا‬ ‫الحباث‬ ‫ية‬‫رص‬‫امل‬ ‫ية‬‫مجلع‬
‫يف‬ ‫هرت‬‫ش‬‫أ‬
19
‫مفرب‬‫و‬‫ن‬
2014
Egyptian Association for Cancer Research
(EACR)

145. Our next goal is to turn into
A Trans-disciplinary team

146. Healthy Team Structure
1.Clear Vision
2.Focused Mission
3.Targeted Objectives
4.Feasible Sustainability
5.Track Record of the Research Team

147. To Be
1. United
2. Patient
3. Optimistic
4. Enthusiastic
5. Open minded
6. Overcome the obstacles
7. Look for new partners

150. Thank you
Thank you