2. You will have to separate three components of paprika.
The three components can be easily identified because they are
colored (absorb visible light).
They have different polarities.
They can be separated using column chromatography.
You can monitor the separation using thin layer chromatography.
What is chromatography….
• Very useful technique in organic chemistry
based on differential adsorption.
• Used to separate components in a mixture
(solid or liquid).
• It depends on the polarity of the ingredients
involved --- intermolecular forces!!
• Thin layer chromatography (TLC) is used to
analyze components and purity of a mixture.
• Thin layer chromatography (TLC) is also used
to monitor the progress of a reaction.
What do we need to perform a
• Adsorbent: Silica gel (silicon dioxide), also
called “stationary phase”.
• Eluent: solvent used to move your compound
trough the silica gel, also called the mobile
• Your compound mixture to be separated.
• Patience and chemical intuition.
• More polar molecules “stick” to the adsorbent longer.
• Less polar molecule separate more easily from the
• When this happens, separation occurs.
To be separated
• More polar solvent move the molecules more efficiently
• Less polar move the molecules less efficiently
• Separation occurs
8. Column Chromatography
Using a Pasteur pipette, load your compound that was dissolved
in a minimum of solvent onto the silica.
Your test solution will then add the eluent.
Do not let your column run dry!!