16. (insect gut) Protein binds to specific receptors Inserts into membrane Forms cation-specific pores DEATH and PARALYSIS
17. Why is there a need to identify the potential pathogenicity of the donor organisms? (relation to ERA) - To identify if it has significant effects to humans, non-target animals
32. For gene flow to occur via normal sexual transmission , certain conditions must exist:
33.
34.
35.
36.
37.
38.
39.
40.
41.
42.
43.
44.
45.
46.
47.
48.
49. Fig. 1. How the high-dose/refuge strategy works to delay the increase in highly resistant (RR) insects in a pest population. Reproduced from Cohen et al. 2000. BT CROP NON-BT CROP 4 essential assumptions:
50.
51.
52.
Notas del editor
There is no evidence that the method used to produce a new plant variety (be it a product of conventional breeding or genetic engineering) can be used as an effective predictor of the plant.s environmental impact or food safety.
To assess the potential for ‘escape’ through pollen, seed or vegetative material
1.Agrobacterium mediated method-Plants transformed independently with the same plasmid will commonly have different levels of expression -T-DNA can integrate into the host genome in patterns other than as a single copy at a single site. Multiple copies in direct or inverted repeats and other complex patterns may also occur. The presence of multimeric T-DNA inserts, especially inverted repeat structures, is strongly linked to the phenomenon of transgene silencing. 2. MPB- the full-length introThe duced transgene truncations. Larger than full length fragments of transgenic DNA may also be caused by interspersion of inserted DNA with host DNA.
One example of an approved event containing a high transgene copy number concerns a line of canola-15 transgene copies