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Principles and Practice of Environmental Safety Assessment of Transgenic Plants
 
[object Object],[object Object],[object Object],[object Object]
[object Object],1. Familiar with the biology of the plant 2. Familiar with the novelty trait 3. Familiar with agricultural/silvicultural practices employed it its cultivation
[object Object],[object Object]
[object Object],Novelty trait of the genetic elements Used to transform it Novelty of the  plant itself
[object Object]
[object Object],[object Object],[object Object],[object Object]
Zea mays
[object Object],[object Object],[object Object],Other than European Corn Borer Control
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],None of them are known to have any Pathogenic characteristics
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],-produce crystalline insecticidal proteins (delta endotoxins) -humans are not affected -no deleterious effects to non-target organisms HOW DOES Cry1Ab WORK?
(insect gut) Protein binds to specific receptors Inserts into membrane Forms cation-specific pores DEATH and PARALYSIS
Why is there a need to identify the potential pathogenicity of the donor organisms? (relation to ERA) - To identify if it has significant effects to humans, non-target animals
[object Object],[object Object],[object Object],[object Object],Plasmid w/ gene of interest Tungsten/gold particles (microcarriers) Host cell (nucleus) DNA may separate with microcarrier Integrate with host genome 1.  Agrobacterium mediated transformation
[object Object],[object Object],[object Object],[object Object]
[object Object]
[object Object],[object Object],[object Object],[object Object],MON 810:
[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],Integron Analysis of  MON 810  -Southern blot analysis: cry1ab insertion   stable in 3 generations of MON 810 breeding
 
TRIALS ANTISERA
[object Object],[object Object],[object Object],[object Object]
 
[object Object],There’s more..
 
 
 
For gene flow to occur via normal sexual  transmission , certain conditions must exist:
 
[object Object],[object Object],Up next: Gene Transfer to unrelated Plants organisms
[object Object],[object Object],[object Object],[object Object]
Up next:  weediness  potential ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
 
[object Object],[object Object],[object Object],[object Object],[object Object],THEREFORE …………………
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Up next: secondary and non-target adverse effects
[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],Field Studies: United States
[object Object],[object Object],#45. Slide 45
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Up next:  Insect Resistance Management
[object Object],[object Object]
[object Object],[object Object],[object Object]
[object Object],[object Object]
Fig. 1. How the high-dose/refuge strategy works to delay the increase in highly resistant (RR) insects in a pest population. Reproduced from Cohen et al. 2000. BT CROP NON-BT CROP 4 essential assumptions:
[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object]

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Srep18078
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Final report mst 5

  • 1. Principles and Practice of Environmental Safety Assessment of Transgenic Plants
  • 2.  
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  • 7.
  • 8.
  • 9.
  • 11.
  • 12.
  • 13.
  • 14.
  • 15.
  • 16. (insect gut) Protein binds to specific receptors Inserts into membrane Forms cation-specific pores DEATH and PARALYSIS
  • 17. Why is there a need to identify the potential pathogenicity of the donor organisms? (relation to ERA) - To identify if it has significant effects to humans, non-target animals
  • 18.
  • 19.
  • 20.
  • 21.
  • 22.
  • 23.
  • 24.  
  • 26.
  • 27.  
  • 28.
  • 29.  
  • 30.  
  • 31.  
  • 32. For gene flow to occur via normal sexual transmission , certain conditions must exist:
  • 33.  
  • 34.
  • 35.
  • 36.
  • 37.  
  • 38.
  • 39.
  • 40.
  • 41.
  • 42.
  • 43.
  • 44.
  • 45.
  • 46.
  • 47.
  • 48.
  • 49. Fig. 1. How the high-dose/refuge strategy works to delay the increase in highly resistant (RR) insects in a pest population. Reproduced from Cohen et al. 2000. BT CROP NON-BT CROP 4 essential assumptions:
  • 50.
  • 51.
  • 52.

Notas del editor

  1. There is no evidence that the method used to produce a new plant variety (be it a product of conventional breeding or genetic engineering) can be used as an effective predictor of the plant.s environmental impact or food safety.
  2. To assess the potential for ‘escape’ through pollen, seed or vegetative material
  3. 1.Agrobacterium mediated method-Plants transformed independently with the same plasmid will commonly have different levels of expression -T-DNA can integrate into the host genome in patterns other than as a single copy at a single site. Multiple copies in direct or inverted repeats and other complex patterns may also occur. The presence of multimeric T-DNA inserts, especially inverted repeat structures, is strongly linked to the phenomenon of transgene silencing. 2. MPB- the full-length introThe duced transgene truncations. Larger than full length fragments of transgenic DNA may also be caused by interspersion of inserted DNA with host DNA.
  4. One example of an approved event containing a high transgene copy number concerns a line of canola-15 transgene copies