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Venipuncture Equipment Unit 2
1. Blood Collection:
A Short Course, 2nd edition
UNIT 2
VENIPUNCTURE
EQUIPMENT
Marjorie Schaub Di Lorenzo, MT(ASCP)SH
Susan King Strasinger, DA, MT(ASCP)
2. ∗ Differentiate between an evacuated tube system,
syringe, and a winged blood collection set for the
collection of blood by venipuncture.
∗ Differentiate among the various needle sizes as to
length, gauge, and purpose.
∗ Discuss methods to safely dispose of contaminated
needles.
LEARNING OBJECTIVES
3. ∗ Identify the types of evacuated tubes by color code,
and state the anticoagulants and additives present,
the mechanism of action, any special characteristics,
and the purpose of each.
∗ List the correct order of draw for the various types of
blood collection tubes.
LEARNING OBJECTIVES
4. ∗ Discuss the purpose and types of tourniquets.
∗ Name three substances used to cleanse the skin prior
to venipuncture.
∗ Discuss the use of gloves, gauze, and bandages when
performing venipuncture.
∗ Describe the quality control of venipuncture
equipment.
LEARNING OBJECTIVES
9. ∗ Evacuated tube system
∗ Syringe system
∗ Winged blood collection set
VENIPUNCTURE METHODS
10. EVACUATED TUBE SYSTEMS
∗ Eliminate transfer of blood
∗ Minimize exposure to blood
∗ Three-part system
11. ∗ Sterile, disposable
∗ Standard gauge, 20–22
∗ 21-22 gauge standard with evacuated tubes
∗ Length, 1 or 1.5 inches
∗ 23 gauge with ¾” length may be used
∗ Children/persons with small veins
NEEDLES
12. ∗ Avoid
∗ Most have eliminated 20-gauge needles
∗ Patients on blood thinner = hematomas
∗ Use of 25-gauge needles
∗ Needle in vein longer
∗ Tube fills more slowly–microclots form
∗ Hemolysis occurs more frequently
NEEDLES
31. DO NOT TRANSFER BLOOD
BETWEEN TUBES
CONTAINING DIFFERENT
ANTICOAGULANTS OR
ADDITIVES.
32. ∗ EDTA is the anticoagulant
∗ Specimen is whole blood
∗ Primary test is the CBC
• Maintains cellular integrity
• Inhibits platelet clumping
• Does not interfere with routine staining procedures
∗ CLSI recommends spray-dried K2EDTA for hematology
tests
∗ Do not use for coagulation tests
LAVENDER TUBES
33. ∗ K2EDTA is the anticoagulant
∗ Specimen is whole blood
∗ Designated tube for blood bank tests
∗ Special label required by the AABB
PINK TUBES
34. ∗ K2EDTA is the anticoagulant with gel
∗ Plasma preparation tube (PPT)
∗ Specimen is plasma
∗ Used for molecular diagnostic tests
WHITE TUBES
35. ∗ Sodium citrate is the anticoagulant
∗ Specimen is plasma
∗ Primarily used for coagulation tests
• Preserves coagulation factors
∗ Ratio of blood to liquid Na citrate = 9:1
• Make adjustments for high and low HCTs
LIGHT BLUE TUBES
38. ∗ Specimen is plasma
∗ Used for fibrin degradation products
∗ Additives are thrombin and soybean trypsin inhibitor
SPECIAL BLUE TUBES
39. ∗ Sodium citrate is the anticoagulant
∗ Blood to liquid anticoagulant is 4:1
∗ Specimen is whole blood
∗ Used for Westergren sedimentation rates
∗ Specially designed tubes
BLACK TUBES
40. ∗ Heparin is the anticoagulant
∗ Specimen is plasma
∗ Chemistry tests
∗ Avoid anticoagulant interference with particular tests
(Na, NH4, Li)
∗ Heparin interferes with differential staining
GREEN TUBES
41. ∗ Plasma separator tubes (PSTs)
∗ Lithium heparin is the anticoagulant
∗ Additive is separation gel
∗ Specimen is plasma
∗ Separates plasma from cells
∗ Ideal for potassium tests and other chemistry tests
LIGHT GREEN/GREEN BLACK TUBES
42. ∗ Potassium oxalate or Na2EDTA may be the
anticoagulant
∗ Antiglycolytic agent, sodium fluoride, is the additive
∗ Specimen may be plasma or serum
∗ Used for glucose and blood alcohol levels
∗ Sodium fluoride interferes with some enzyme tests
∗ Potassium oxalate distorts RBC morphology
GRAY TUBES
43. ∗ Plain with clot activator, sodium heparin, or
K2EDTA
∗ Specimen may be serum or plasma
∗ Specially formulated rubber stoppers
∗ Chemically clean tubes
∗ Used for trace elements, toxicology, and
nutrients
ROYAL BLUE TUBES
44. ∗K2EDTA is the anticoagulant
∗Specimen in plasma
∗Contain less than 0.01 µg/mL lead
∗Used for lead testing
TAN TUBES
45. ∗Blood bank
• Acid citrate dextrose (ACD)
∗ Preserves red blood cells
∗Microbiology
• Sodium polyanetholsulfonate (SPS)
• Blood cultures
∗ Aids microorganism recovery
YELLOW TUBES
46. ∗Thrombin is the additive
∗Specimen is serum
∗Used for STAT serum chemistry
tests
ORANGE/YELLOW GRAY TUBES
47. GOLD/RED-GRAY (SST) TUBES
∗ Additives
∗ Silica clot activator
∗ Thixotropic separation gel
∗ Specimen is serum
∗ Allow to clot for 30 minutes
∗ Used for chemistry tests
∗ Do not use for blood bank or
some immunology/serology tests
49. ∗Silica clot activator is the additive
∗Specimen is serum
∗Used for serology, chemistry, and
blood bank testing
RED PLASTIC TUBES
50. ∗ No anticoagulants or additives
∗ Specimen is serum
∗ Allow to clot for 60 minutes
∗ Used for chemistry, serology, and blood bank testing
RED GLASS TUBES
51. ∗ No additives or anticoagulants
∗ Used as a discard tube
CLEAR/RED-LIGHT GRAY TUBES
54. ∗Tissue thromboplastin affecting
coagulation tests
∗Transfer of EDTA to a tube for
calcium testing
∗Transfer of Na citrate or K oxalate
to a tube for electrolyte testing
EXAMPLES
55.
56. CLSI-RECOMMENDED ORDER OF
DRAW
∗ Blood culture
∗ Light-blue
∗ Red/gray, gold, red plastic or glass
∗ Light green PST, green
∗ Lavender, pink, white
∗ Gray
∗ Yellow/gray, orange
57. ∗Used for small, fragile veins
∗Suction pressure on vein can be
controlled
∗Coordinate syringe size with
volume of blood required
SYRINGES
65. COMBINATION SYSTEMS
∗ Blood collection tube and
collection device are combined in
one unit
∗ Blood collected by either
evacuated tube or syringe
technique
∗ Use regular needles or winged
blood collection needles
77. ∗Short draws
• Excessive dilution by liquid anticoagulant
• Underfilled sodium citrate tubes cause a falsely lengthened
aPTT result
• Distortion of cellular structures
• Underfilled EDTA tubes cause red blood cell shrinkage
∗Avoid manual filling of additive
tubes
POSSIBLE ERRORS