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  1. 1. SEMINARONSTRUCTURE OF ANTIBODYPRESENTED BYPREETI MADHARIAM.Sc. PREVIOUS (BIOTECHNOLOGY)
  2. 2. CONTENTSINTRODUCTIONHISTORYPROPERTIES OF ANTIBODYENZYMATIC EXPERIMENTS WITH ANTIBODYSTRUCTURE OF ANTIBODYTYPES OF ANTIBODYFUNCTIONS OF ANTIBODYCONCLUSIONREFERENCES
  3. 3. INTRODUCTION Antibodies are the antigens binding proteins presenton B cell membrane and secreted by plasma cells. Antibodies are active serum proteins formed inresponse to an antigen and react specifically withthat antigen.HISTORY Antibodies were contained in particular serum protein fractionscame from a classic experiment by A.Tiselius and E.A. Kabat in1939. Rodney Porter in 1962 proposed the basic structure of antibody.
  4. 4. PROPERTIES OF ANTIBODY Antibody is a glycoprotein. Antibody is a “Y” shaped molecules. Antibody is made up of four polypeptide chain. Antibody is heterodimeric structure. All antibodies are immunoglobulins ,but allimmunoglobulins may not be antibodies. Antibodies contain disulphide bonds.
  5. 5. ENZYMATIC EXPERIMENT WITH ANTIBODYB- marcaptoethanol
  6. 6. STRUCTURE OF ANTIBODYHingeregionAntibody DomainsVHVCFabFcDisulphideBondVC
  7. 7. TYPES OF ANTIBODY(PENTAMER)(DIMER)(MONOMER)(MONOMER)(MONOMER)Light chainHeavy chain
  8. 8. IgGIgG1IgG2 IgG3IgG4TYPES OF I Gg
  9. 9. FUNCTIONS OF ANTIBODIES The main function of antibody is protect the body against antigen. The protective role is carried out into different ways-1. By Direct :-a) Precipitation : - Antibody combines with soluble antigensand form a lattice is known asprecipitation reaction.b) Agglutination :- The interaction between antibody and aparticulate antigens result in visibleclumping is known as agglutination.c) Opsonisation :- A process by which Phagocytotic cell isfacilated by deposition of opsonin isknown as opsonisation.
  10. 10. 2. By Indirect :-a) Activation of complement system :- Complement is aset of plasma protein that is activated by bindingantigens.b) Antibody dependent cell mediated cytotoxicity(ADCC):-It is a form of lymphocytes mediated cytotoxicity inwhich an effector kills an antibody coated target cell.
  11. 11. CONCLUSIONAntibody is a glycoproteins which helps toimmune system. It has an antigen binding sitethrough which it is attached to the foreign particlesor antigens like bacteria, viruses etc and degradethem.
  12. 12. REFERENCES Kuby J. Antibodies: Structure and functions(pg. 76-101) Immunology 5th ed. Fatima D. Immunoglobulins (pg.81-99), Saraspublication Websites:www.qwickstep.comwww.biochemistry4all.comwww.dels-old.nas.eduwww.medicine.virginia.eduwww.goodpsych.com
  13. 13. THANK YOU

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