4. Target
Monitoring
Sites
• Dublin
sites
have
been
changed
and
an
extra
site
has
been
included:
– Lucan
Bridge,
Malahide,
Kilcullen
Bridge
and
Poobeg
• This
sampling
will
be
taking
place
from
May
2014.
• Historical
data
from
MI
and
DCU
is
available
for
these
sites.
5. Target
Monitoring
Sites
• Upstream
site
added
to
cypermethrin
study.
• SPMD,
POCIS,
grab
samples
and
biota
to
be
collected.
• Patricia
McCreesh
and
Ray
Thomas
supplied
informaNon
on
historic
data
and
site
selecNon
for
Donegal.
6. Protocol
for
Biota
Sampling
• 50
My#lus
edulis
mussels
• 4
–
6
cm,
4
–
5
cm
• Depurate
for
24
hours
• Dissect
(shell
weight,
Nssue
weight)
• Homogenise
• Store
at
-‐30C
7. Literature
Review
• ‘Passive
Sampling
of
Emerging
Compounds’
– Environmental
PolluNon
(IF
3.730)
8. Deliverables
Work
Package
1
Status
Desk
study
and
literature
review
Complete
SelecNon
of
target
monitoring
staNons
Complete
Develop
protocols
and
select
locaNons
for
biota
monitoring
Complete
Develop
detailed
protocols
for
passive
sampler
deployments
Complete
Final
Report:
Desk
study
with
protocols
for
deployment,
biota
sampling
10. Work
Package
2
:
• Method development ongoing for:
– Organohalogen pesticides by GCMS
– HBCD and BFRs by LCMS
– PFOS by LCMS
– Pharmaceuticals and endocrine disrupting compounds
• Extraction by soxhlet/solid phase extraction (SPE)
• Analysis by gas and liquid chromatography with
tandem mass spectrometric or UV detection
11. Deliverables
Work
Package
2
Status
Method
selecNon
and
opNmisaNon
Methods
in
place
for
24
of
27
analytes
Methods
for:
Organohalogen
pesNcides,
HBCD.
Transfer
of
BFR
method
to
LC-‐MS/MS
Ongoing
Methods
for:
EDCs
Ongoing
Methods
for:
PharmaceuNcals
Ongoing
Methods
for:
PFOS
Ongoing
Note: All methods to be operational by month 26.
13. Work Package 3:
• Catchment approach has been taken in
Cork
• Deployment of POCIS and PDMS samplers
in July 2013
• POCIS collected after 4 weeks
• PDMS and water samples collected after 2
months
14. Sampling
• Second
sampling
regime
in
Cork
underway
for
PDMS
and
POCIS.
• POCIS
trial
to
Ne-‐in
with
WP
3
in
Cork
– 2,
4,
6
week
collecNons
to
evaluate
uptake
• Samplers
deployed
in
Kilkieran
(March
2014),
POCIS
and
grab
samples
collected.
16. Deliverables
Work
Package
3
Status
Prepare
equipment
(PDMS
&
POCIS)
for
deployment
Complete
Deployment
of
samplers
Phase
two
complete
CollecNon
and
screening
analysis
Ongoing
Spot
sampling
as
compliance
measure
Phase
two
complete
18. Biota
sampling
• Fish
samples
supplied
by
IFI
for
Cork
catchment
(2013)
– Trout
(Upper
Lee
catchment)
– Whole
fish
• Analysed
for
organic
pollutants
at
the
MI
– Ongoing
• IFI
to
supply
more
samples
in
2014
• Mussels
collected
from
marine
sites
19. Deliverables
Work
Package
4
Status
Sampling
programme
for
biota
at
all
locaNons
Ongoing
Sample
collecNon
&
preparaNon
Ongoing
Analysis
of
shellfish
and
fish
samples
Ongoing
RecommendaNons
Cork
PDMS
redeployed
22. County
Site
RaNonale
POCIS
PDMS
Water
Mussels
Fish
(IFI)
Cork
Inchigeelagh
Upstream
river
staNon
✓
✓
✓
✓
Inniscarra
Downstream
river
staNon
✓
✓
✓
✓
Shandon
Riverine/transiNonal
✓
✓
✓
✓
Lough
Mahon
Riverine/transiNonal
staNon
✓
✓
✓
✓
Outer
bay
Riverine/transiNonal
staNon
✓
✓
✓
✓
Dublin
Malahide
High
pressure
coastal
✓
✓
✓
✓
Poolbeg
Riverine/transiNonal
✓
✓
✓
✓
Lucan
Bridge
Downstream
river
staNon
✓
✓
✓
✓
Kilcullen
Bridge
Upstream
river
staNon
✓
✓
✓
✓
Galway
Kilkieran
Bay
Coastal
reference
staNon
✓
✓
✓
✓
Mayo
Burrishoole
Upstream
river
staNon
✓
✓
✓
✓
Donegal
Glen
Lackagh
1
Cypermethrin
study
SPMD
✓
✓
EPA
Benthic
kick
sampling
Glen
Lackagh
2
Cypermethrin
study
SPMD
✓
✓
23. Work
Package
5
:
• Cypermethrin study beginning April 2014
• Analysis by Ray Thomas, NIEA
• Site (s):
– Glen Lackagh sites 38G040900 and 38G040700
upstream of Glen Lough, Donegal
– Pre- and post- application study
24. Cypermethrin
Data
River
Q-‐Value
Date
X
Y
Remarks
from
fieldsheets
GLEN
(LACKAGH)
2-‐3
28/09/2012
212685
431467
InvesNgaNve.
Toxic
signs
at
0900.
Worse
here.
Sheep
suspected
–
cypermethrin
EffecNvely
a
dead
river
here.
Some
forestry
u/s.
Also
one
field
that
looks
''burned''
possible
herbicide?
Lots
of
heather
u/s.
didn''t
see
sheep
on
open
land.
Okay
at
0400
GLEN
(LACKAGH)
3/0
26/09/2012
211832
431043
Something
wrong
here.
Two
samples
taken.
Very
sparse.
Sheep
dip?
25. Cypermethrin
Assessment
• Large
dataset
of
usage
and
occurrence
reports
has
been
compiled.
– Proposed
desk
study
on
this
data
• InformaNon
from
census
of
agriculture,
department
of
veterinary
medicine,
Coilte,
Teagasc,
DEFRA,
department
of
Agriculture,
etc.
• Ray
Thomas,
Patricia
McCreesh
and
Danielle
Ashton
have
assisted
the
project
team
in
the
collecNon
of
this
data.
26. Deliverables
Work
Package
5
Status
Site
selecNon
and
sampler
deployment
Sites
selected,
deployment
ongoing
Spot
sample
collecNon
Ongoing
Analysis
of
samples
for
cypermethrin
May/June
2014
Passive
sampler
extracNon
and
analysis
for
cypermethrin
June
2014
Assessment
of
upland
acNvity
Ongoing
28. Work
Package
6
:
• WWTP outfall study-EDCs and pharmaceuticals
• Methods
– In place for E2 in water and biota
– Optimising method for EE2 to detect below EQS
– Diclofenac method under development
• POCIS devices suitable
– Issue with sampling rates
• MI experience with integrated study-historical data
for E2 and EE2 in Dublin
29. Deliverables
Work
Package
6
Status
Site
selecNon
and
sampler
deployment
Ongoing
Spot
sample
collecNon
Ongoing
Analysis
of
samples
for
EDCs
and
PharmaceuNcals
Ongoing
Passive
sampler
extracNon
and
analysis
for
EDCs
and
PharmaceuNcals
Ongoing
CollecNon
of
background
data
on
WWTP
efficiency
Ongoing
31. PS
and
biota
• Biota
EQS
set
for
11
compounds
• EU
guidance
document
currently
being
developed
– SelecNon
of
target
organism
differs
between
MS
– Human
health
vs
secondary
poisoning
– Require consistency and comparability to assess compliance against
the EQSbiota and in interpretation of data
• PS
and
biota
– Similar
drivers
for
uptake
– Environmental
factors
can
be
modelled
– Metabolism
and
health
status
can
be
complicaNng
factors
– Suitable
where
no
biota
are
present
32. White PhD thesis: Relationship between BAFs (dry weight) calculated from mussels and PS at the Kilkieran reference site vs. parameter LogKpw.
1
2
3
4
5
6
7
3
3.5
4
4.5
5
5.5
6
6.5
7
7.5
Log
BAF
(dry
weight)
Log
Kpw
PAH
BAFdw
PCB
BAFdw
OC
BAFdw
PBDE
BAFdw
Log BAFdw = 0.80 (Log Kpw) + 0.13 R2= 0.75
BAF
model
for
PDMS
and
biota
(mussels):
Kilkieran
34. Project
Management
• Monthly and weekly meetings and conference
calls have continued for the project team.
• Monthly analytical reports have been
introduced to facilitate the final STRIVE report
deliverable due at project completion.
35. Funding
OpportuniQes
PublicaQons
in
PreparaQon
• JPI Water call
• Passive sampling review (submitted)
• Book (August 2014)
• Review chapter (May/June 2014)
36. Deliverables
Work
Package
3
Status
Kick-‐off
meeNng
Complete
Workshops
1/3
Project
meeNngs
Ongoing
Financial
and
Technical
reports
(
EPA
Deadlines)
Ongoing
Monthly
communicaNons
Ongoing
Final
Report
2016
37. Acknowledgements
This project is funded by the EPA as part of the Science,
Technology, Research and Innovation for the Environment
(STRIVE) Programme 2007–2013. This programme is financed by
the Irish Government under the National Development Plan 2007–
2013.
It is administered on behalf of the Department of the Environment,
Heritage and Local Government by the Environmental Protection
Agency, which has the statutory function of co-ordinating and
promoting environmental research.
