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# Two compounds- A and B- were separated by gas liquid chromatography-.docx

Two compounds, A and B, were separated by gas â€“ liquid chromatography. The retention times are 628 s and 642 s, and the widths at half-height are 8.3 s and 9.4 s, correspondingly. Find the resolution for these two compounds. Are the peaks well resolved?
Solution
Resolution in HPLC is Rs=2(tR2-tR1)/(W1+W2)
Where tR=retention time, W=peak width
Given tR1=628 s, tR2=642 s, W1=8.3 s, W2=9.4 s,
Rs=2x(642s - 628s)/(8.3s + 9.4s)
Rs=1.58.
The peaks are well resolved, since ifÂ Â R > 1.5, then peaks are completely resolved.
Here Is=1.58 > 1.5, then peaks are well resolved.
Thanks and I hope you like it. Please let me know if you have any doubt.
.

Two compounds, A and B, were separated by gas â€“ liquid chromatography. The retention times are 628 s and 642 s, and the widths at half-height are 8.3 s and 9.4 s, correspondingly. Find the resolution for these two compounds. Are the peaks well resolved?
Solution
Resolution in HPLC is Rs=2(tR2-tR1)/(W1+W2)
Where tR=retention time, W=peak width
Given tR1=628 s, tR2=642 s, W1=8.3 s, W2=9.4 s,
Rs=2x(642s - 628s)/(8.3s + 9.4s)
Rs=1.58.
The peaks are well resolved, since ifÂ Â R > 1.5, then peaks are completely resolved.
Here Is=1.58 > 1.5, then peaks are well resolved.
Thanks and I hope you like it. Please let me know if you have any doubt.
.

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