Introducing New Genes Into Crops

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Recombinant DNA (r-DNA) technology

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genetic engineering, principles, b pharma 6th sem, biotechnology What is a gene ? Definition History Process Molecular tools of genetic engineering Restriction enzymes History of restriction enzyme Mechanism of action Types of restriction enzymes Application of restriction enzymes Blunt ends Sticky ends transgenic cisgenic. knockout organism. Host organism vector TRANSGENIC PLANTS DOLLY THE SHIP TRANSGENIC ANIMALS

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This technique helps in maintaining plant tissues for prolonged periods on an artificial culture/basal medium. Small group of cells is isolated and inoculated on a culture medium comprising carbohydrate source, vitamins, minerals and defined nitrogenous source(s) under sterilized lab conditions. It is possible to cultivate any plant species using variable explants e.g. stem segment, leaf segment, pedicel, petiole, anther, pollen, microspores, petiole, cotyledons, endoperm, etc. Callus derived from a single initial group of cells can be sub-cultured, multiplied and in some instances induced to reinitiate differentiation of roots and shoots and thus form plantlets (Fig. 30-1). The latter are hardenend and then transferred to the soil.

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