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M-finite applications of the M-Series (MRI) Presentation​.pptx

The document provides an overview of Scintica's M-Series compact MRI systems designed for pre-clinical research, highlighting their design, user-friendly interface, and various applications in anatomy, neurology, cancer biology, and cardiovascular studies. The M-Series requires minimal infrastructure, operates quietly, and can produce high-quality imaging for a range of experimental needs. It also emphasizes the integration of advanced software for efficient imaging protocol management and data analysis.

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WWW.SCINTICA.COM Footer 1 1 WWW.SCINTICA.COM M-finite applications of the M-Series Compact, Self-Shielded, High Performance, Small Animal MRI
WWW.SCINTICA.COM Outline • M-Series Magnet Design and User Experience • Poll Question • Application Areas • Anatomy & Morphology • Neurology • Cancer Biology • Cardiovascular Biology • Contrast Agents • Multi-Modal Imaging • Ex Vivo Imaging Footer 2
WWW.SCINTICA.COM M-SeriesTM Magnet Design • Requires no special infrastructure or cooling • Compact and self-shielded with minimal external fringe field • Easily installed within an animal facility, or existing laboratory next to other equipment or furnishings • Operates very quietly during image acquisition • Systems are installed with first images being acquired in less than 1 day 3
WWW.SCINTICA.COM M-Series (M5) Components Footer 4 • Electronics Cabinet and User Workstation • Compact Magnet • Animal Handling System • Animal handling systems for mice and rats
WWW.SCINTICA.COM Animal Handling System • Fully integrated animal handling and coil system includes: • Mouse or rat beds to suite a variety of sized animals • Anatomy specific coils • Animal heating • Physiological monitoring • Anesthesia delivery and scavenging 5
WWW.SCINTICA.COM Animal Preparation and Imaging Set-Up 6
WWW.SCINTICA.COM Software Interface and User Experience • The M-Series software is intuitive, and easy to operate; designed to quickly generate reproducible, and quantitative results • No need to have a background in MR physics to operate the system – default sequences are available and can be further optimized • Experienced MR users have full flexibility to customize options to tailor the performance of the system to meet their needs • Software is study based, and optimized protocol sets may be selected by the user 7
WWW.SCINTICA.COM M-Series Systems • The M-Series are designed with the pre- clinical researcher in mind • No MRI technician needed • Streamlined workflow for animal preparation and positioning • User-friendly software interface • Wide variety of applications Footer 8
Audience Poll
WWW.SCINTICA.COM Key Research Applications • Anatomy and Morphology • Neurology • Cancer Biology • Cardiovascular Biology • Contrast Agents • Multi-Modal Imaging • Ex Vivo Imaging 10
WWW.SCINTICA.COM Anatomy and Morphology: Normal Mouse 11 T2 weighted FSE on Mouse Abdomen T1 weighted SE on Mouse Abdomen 400µm resolution 4:54m:s, 7 excitations 420µm resolution 6:12m:s, 11 excitations
WWW.SCINTICA.COM Anatomy and Morphology: Spinal Imaging Footer 13 Fixed mouse T1 weighted imaging Live mouse T2 weighted imaging Live mouse T2 weighted imaging
WWW.SCINTICA.COM Anatomy and Morphology: Hind Limb Inflammation • Acute inflammation was induced by topical application of an irritant • Lesion volume (red) = 486mm3 • Entire ipsilateral leg volume (blue+red) = 1120mm3 • Contralateral leg volume (green) = 824mm3 14 T2 weighted: SE(TE/TR=50/1500, FOV=60mm, Matrix=256x256, Res. 235um, Acq. Time 6:24m:s)
WWW.SCINTICA.COM Anatomy and Morphology: Visceral Fat Segmentation • Images can be automatically segmented based on grey- scale intensities of connected voxels – in these T1 weighted images adipose tissue appears very bright • Volume = 1075mm3 15
WWW.SCINTICA.COM Key Research Applications • Anatomy and Morphology • Neurology • Cancer Biology • Cardiovascular Biology • Contrast Agents • Multi-Modal Imaging • Ex Vivo Imaging 16
WWW.SCINTICA.COM Neurology: Normal Mouse 17 T2 weighted: FSE Coronal/Sagittal 120um, 12:20m:s; Axial 160um, 16:00m:s
WWW.SCINTICA.COM Neurology: Glioblastoma (UIC training) Footer 18 Glioblastoma model with frequent edema Fluid attenuation T2 weighted imaging (FLAIR) T2 weighted imaging T2 weighted imaging Edema in red Edema in red Tumor in blue T2 weighted: FSE Axial 250um, 6:44m:s Images courtesy of Dr. David Largaespada’s group at University of Minnesota
WWW.SCINTICA.COM Neurology: Orthotopic Glioblastoma • Day 4 – normal anatomical structures are visible • Day 15 – tumor is visible, spread throughout the brain, enlarged ventricles • Tumor volume = 20mm3 19 T2 weighted: FSE (TE/TR=73.8/3100, FOV=40x20mm, Matrix=256x128, NEX=20, ETL=16, Res. 156um, Acq. Time 10 min) 4 days post injection 15 days post injection
WWW.SCINTICA.COM Neurology: Traumatic Brain Injury • TBI caused by percussion injury to the skull • Injury appears clearly on a T2 weighted image due to the inflammatory lesion • Effect of preventative measures or therapeutic response can be evaluated 20 T2 weighted: FSE (TE/TR=74/2840, FOV=50, Matrix=256x256, Res. 195µm, Acq. Time 13:46 min:sec) Images courtesy of Prov. A. Friedman & S. Lublinsky – Brain Imaging Research Center, Ben-Gurion University of the Negev
WWW.SCINTICA.COM Neurology: Epilepsy • Epilepsy was induced by intoxication with paraoxone causing severe cholinergic symptoms • Significant changes are visible in the cortex 48 hours post exposure 21 T2 weighted: FSE (TE/TR=74/3400, FOV=50, Matrix=256x256, Res. 195µm, Acq. Time 16:30 min:sec) Images courtesy of Prov. A. Friedman & S. Lublinsky – Brain Imaging Research Center, Ben-Gurion University of the Negev 3 hours post PO exposure 48 hours post PO exposure
WWW.SCINTICA.COM Neurology: Stroke • Stroke was induced by photothrombosis in the rat brain • The stroke lesion is clearly visible on T2 weighted images due to the inflammation in the area 22 T2 weighted: FSE (TE/TR=74/3030, FOV=50, Matrix=256x256, Res. 195µm, Acq. Time 14:41 min:sec) Images courtesy of Prov. A. Friedman & S. Lublinsky – Brain Imaging Research Center, Ben-Gurion University of the Negev
WWW.SCINTICA.COM Neurology: Stroke • Stroke volume was calculated in VivoQuant, and found to be 19.0mm3 23 Images courtesy of L. Lacovitti’s lab at Thomas Jefferson University, Hospital for Neuroscience • Stroke was induced by photothrombosis in the mouse brain • The stroke lesion is clearly visible on T2 weighted images due to the inflammation in the area
WWW.SCINTICA.COM Key Research Applications • Anatomy and Morphology • Neurology • Cancer Biology • Cardiovascular Biology • Contrast Agents • Multi-Modal Imaging • Ex Vivo Imaging 24
WWW.SCINTICA.COM Cancer Biology: Tumor Detection Fat-suppressed T2-weighed MRI can be used to monitored tumour burden over time using the same animal as its own control 25 Fat-suppressed T2 weighted: FSE (TE/TR=10-60/4000ms, Inversion time = 100ms) Th-MYCN GEMM 2min scan 8min scan TRAMP GEMM 8min scan O.t. medulloblastoma 3min scan KPC GEMM O.t. Prostate bone marrow metastasis 12min scan RH-41 xenograft 4min scan
WWW.SCINTICA.COM Cancer Biology: Tumour Response Assessment in GEM model of neuroblastoma 26 Fat-suppressed T2 weighted: FSE (TE/TR=10/4500ms, FOV=60x30mm, slice thickness 1 mm, Matrix=144x72, NEX=2, Inversion time = 100ms, total scan time <2min) • Rapid screening: Mouse is back in its cage within 5mins • Reduced bias: Compared to physical palpation • Reduction in animal used: enhanced statistical power • Enhanced data curation • Nijhuis A et al., Nat Commun. 2022 • Poon E et al., J Clin Investig. 2020 • Dubiella C et al., Nat Chem Bio. 2021 • Roeschert C et al., Nat Cancer. 2021 183 mm3 426 mm3 593 mm3 29 mm3 277 mm3 867 mm3 143 mm3 0 mm3
WWW.SCINTICA.COM Cancer Biology: Flank tumor (UIC training) 27 T1 weighted imaging T2 weighted imaging Images courtesy of Dr. David Largaespada’s group at University of Minnesota
WWW.SCINTICA.COM Cancer Biology: Xenograft Tumor Model • Subcutaneous head and neck tumor located on the hind limb • Tumor can be identified with clear borders on the T2 weighted image – images taken 3 weeks post implantation • Internal structures, such as cysts and lobes, can easily be seen • Tumor volume is easily quantified = 730mm3 28 T2 weighted: FSE (TE/TR=52.7/3500, FOV=80x30mm, Matrix=256x96, NEX=8, ETL=16, Res. 312um, Acq. Time 4:40 min) Model Courtesy of Dr. J. Mahmood, PhD., Radiation Medicine Program, Princess Margaret Cancer Center, UHN
WWW.SCINTICA.COM Cancer Biology: Nerve Sheath (UIC training) 29 Images courtesy of Dr. David Largaespada’s group at University of Minnesota
WWW.SCINTICA.COM Cancer Biology: Orthotopic Cervical Tumor Model Therapeutic effect can be monitored over time using the same animal as it’s own control 30 T2 weighted: FSE (TE/TR=52.7/3500, FOV=80x30mm, Matrix=256x96, NEX=8, ETL=16, Res. 312um, Acq Time 4:40 min) T1 weighted: SE (TE/TR=9.8/500, FOV=80x30mm, Matrix=256x96, NEX=3, Res. 312um, Acq Time 2:42min:sec) Model Courtesy of Drs. Naz Chaudary, Richard Hill & Shawn Stapleton, Princess Margaret Cancer Center 0 50 100 150 200 250 300 350 5.5 Weeks 7 Weeks Tumor Volume (mm 3 ) Control Treated Control (n=4) Treated (n=30) (n=30) 5.5 weeks weeks 179±46 mm3 93±8.5 mm3 7 weeks 227±64 mm3 134±11 mm3 Control Treated
WWW.SCINTICA.COM Cancer Biology: Multi modal detection of orthotopic model of prostate bone marrow metastasis 31 Fat-suppressed T2 weighted: FSE (TE/TR=36/7300ms, FOV=60x30mm, slice thickness 0.8 mm, Matrix=240x120, NEX=8, Inversion time = 100ms, total scan time 12min) Control Injected (right leg) Bioluminescence imaging Maximum intensity projection (MIP) (3 slices) Control Injected
WWW.SCINTICA.COM Key Research Applications • Anatomy and Morphology • Neurology • Cancer Biology • Cardiovascular Biology • Contrast Agents • Multi-Modal Imaging • Ex Vivo Imaging 32
WWW.SCINTICA.COM Cardiovascular Biology: CINE Imaging 33 Images courtesy of Erik Blackwood at the Translation Cardiac Research Center The University of Arizona
WWW.SCINTICA.COM 34 Data from short axis For the Left Ventricle: o End Diastole and End Systole LV mass (ED/ED LVM), in g o End Diastolic Volume (EDV), in ml o End Systolic Volume (ESV), in ml o Stroke Volume (SV), in ml o Ejection Fraction (EF), as % o Cardiac Output, in l/min, when the user inputs the heart rate o Heart Rate, in bpm, as input by the user For the Right Ventricle: o End Diastole and End Systole LV mass (ED/ED LVM), in g o End Diastolic Volume (RVEDV), in ml o End Systolic Volume (RVESV), in ml o Stroke Volume (RVSV), in ml o Ejection Fraction (EF), as %
WWW.SCINTICA.COM Strain Analysis-Long Axis (CINE) 35
WWW.SCINTICA.COM Strain Analysis-Exported data 36
WWW.SCINTICA.COM Angiography – TOF 2D Footer 37 Mouse: Brain Mouse: Cardiovascular system Mapping of blood vessels in the body without the use of contrast agents
WWW.SCINTICA.COM Key Research Applications • Anatomy and Morphology • Neurology • Cancer Biology • Cardiovascular Biology • Contrast Agents • Multi-Modal Imaging • Ex Vivo Imaging 38
WWW.SCINTICA.COM Contrast Agent Imaging • 50µL Primovist (Gd-EOB- DTPA), was injected i.v. through the tail vein • T1 weighted imaging was performed pre and post contrast injection 39
WWW.SCINTICA.COM Contrast Agent Imaging • VivoQuant was used to subtract the pre and post contrast injection images, the results were colored green 40
WWW.SCINTICA.COM Contrast Agent Imaging: Normal Mouse • Dynamic Contrast Enhanced imaging was performed during the injection – 1 frame/sec 41
WWW.SCINTICA.COM Key Research Applications • Anatomy and Morphology • Neurology • Cancer Biology • Cardiovascular Biology • Contrast Agents • Multi-Modal Imaging • Ex Vivo Imaging 42
WWW.SCINTICA.COM Multi-Modal Imaging: PET/MRI • The SimPET insert expands the capabilities of the M7 system to allow for simultaneous PET/MR imaging • MR images compliment the highly sensitive PET images in detecting functional information, abnormalities, and early disease, providing anatomical context 43
WWW.SCINTICA.COM Multi-Modal Imaging: PET/MRI • Reconstructed images of a hot rod phantom with rod diameters of 0.75, 1.00, 1.35, 1.70, 2.00, 2.40mm • Reconstructed using 3D OSEM algorithm with incorporation of point spread function (a) or without point spread function (b) • Example image: effects of tumor associated macrophages on tumor hypoxia and aerobic glycolysis (Cancer Research, 2019) 44
WWW.SCINTICA.COM Multi-Modal Imaging: PET/MRI – Tumor Imaging • Tumor showed increase metabolism on FDG-PET compared to contralateral muscle (ratio = 2.7) • Central region of tumor showed decreased PET signal, T2 weighted MR image indicates increased fluid content – possibly a necrotic core • CT images may provide additional anatomical context 45 Model courtesy of Dr. R. DeSouza, STTARR (UHN) PET PET + CT + MRI MRI – T1w CT MRI – T2w Necrotic Core Tumor Tumor volume is best measured on MRI = 410 mm3
WWW.SCINTICA.COM Complimentary Nature of Imaging Modalities Bioluminescence helps to confirm viability of the tumor cells, as they express luciferase, approximate volumes may be possible from the BLI signal; anatomical images help to confirm tumor volume - ultrasound (263mm3) or MRI (273mm3) 46 Orthotopic Mammary Fat Pad Tumor (MDA-MB-231) Optical Imaging - BLI Ultrasound MRI
WWW.SCINTICA.COM Key Research Applications • Anatomy and Morphology • Neurology • Cancer Biology • Cardiovascular Biology • Contrast Agents • Multi-Modal Imaging • Ex Vivo Imaging 47
WWW.SCINTICA.COM Ex Vivo Imaging: MR-Based Histology • High resolution images of an ex vivo fixed rat brain sample • Exquisite details of the structures within the brain can be visualized, identified, and quantified 48 Sample Courtesy of Prof. Alan Johnson- Duke University, NC Image Resolution: 83x83x300 µm
WWW.SCINTICA.COM Ex Vivo Imaging: MR-Based Histology • Toxicology studies rely on a few histological samples taken throughout an organ to look for lesions, i.e. liver toxicity • MR-based histology is performed on intact fixed samples, providing a full 3D image of an organ • Lesions are identified, counted, and volume calculated • MR images may be used to guide tissue sectioning to confirm lesion characteristics using conventional histology 49
WWW.SCINTICA.COM Key Research Applications • Anatomy and Morphology • Neurology • Cancer Biology • Cardiovascular Biology • Contrast Agents • Multi-Modal Imaging • Ex Vivo Imaging 50
WWW.SCINTICA.COM M-Series Systems • The M-Series are designed for pre-clinical researchers • No MRI technician needed • Streamlined workflow for animal preparation and positioning • User-friendly software interface • Wide variety of applications that can contribute to your research Footer 51 WWW.SCINTICA.COM
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