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Photos of Rio Tapajós in
Farm Maloquinha
Itaituba Para
Amazon Brazil
Identification of the positively selected genes governing host-pathogen arm r...
Bacterial evolution is due to the adaptive nature of the core bacterial genomes that plays critical role in diversification, fitness and adaptation of the species to different environment and host. Since Vibrio cholerae represents an appropriate model organism for studying the interplay of environment and host driven factors shaping the microbial genome structure and function, the current study aims to identify genes that are under these strong forces in V. cholerae. Here, we employed a comparative genomics approach to identify genes that are under positive selection in ten strains of Vibrio sp. including four pathogenic V. cholerae strains. From the available genome sequence data, a total of 422 orthologous genes were identified by reciprocal BLAST best-hit method, recombination breakpoint frequency analysis and tree comparison method. These 422 genes, representing the core genome of Vibrio sp., constituted the dataset to be analyzed for evolutionary selections. The analysis of natural selection, based on Maximum Likelihood method on synonymous and non-synonymous substitution rate, confirms the hypothesis that the bacterial core genomes are mostly under purifying selection with a few positively selected regions. However, our finding also reveals that positively selected sites in the Vibrio genome occur in a wide range of different genes encompassing diverse functional pathways including cell surface proteins (e.g. outer membrane-specific lipoprotein transporter/assembly proteins etc.), cell motility proteins (e.g. flagellar motor switch proteins, flagellar hook and assembly proteins), nutrient acquisition (e.g. amino acid, carbohydrate and phosphate ABC transporters), DNA repair and transcription related proteins. Interestingly, these positively selected gene products are directly involved with host-pathogen interactions and fitness in gastrointestinal environment. Therefore, the collective evidences of these positively selected genes spanning several pathways raise the possibility of their involvement in evolutionary arms races with other bacteria, phages, and/or the host immune system. This finding points to the natural selections which is the responsible factor for the diversification of Vibrio genus.
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The document discusses the structure and function of skeletal muscle. It describes the hierarchy of muscle organization from the whole muscle down to the sarcomere level. Key components of muscle fibers and sarcomeres such as myofibrils, actin, and myosin are defined. The process of muscle contraction is summarized in three steps: 1) an action potential causes calcium release and binding to troponin, 2) myosin binds to actin and generates force through its power stroke, and 3) relaxation occurs when calcium is reabsorbed and the sarcomere returns to its resting state. Muscle fiber types and their roles in athletic performance are also overviewed.
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Identification of the positively selected genes governing host-pathogen arm r...
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Cáncer de células pequeñas de pulmón en geriatría definitivo
Cáncer de células pequeñas de pulmón en geriatría definitivo