Laminin is known to play a role in the movement and organisation of embryonic cells into tissues during development. The laminin α-1 protein is encoded by the LAMA1 gene in humans. The laminin α-1 polyclonal antibody detects endogenous levels of laminin α-1 protein.

1. HOW DID OUR
ANTIBODY PERFORM?
ANTIBODY CUSTOMER REVIEW: Laminin a-1 Polyclonal Antibody (STJ93888)

2. What was used?
Primary antibody: STJ93888 Laminin a-1
Provider: St John's Laboratory Ltd.
Dilution ratio: 1:50
Application: Immunofluorescence staining
Materials for validation: Decellularised cornea
Click for product data sheet PDF

3. What Was The Protocol?
Treatment of Materials Tissue was sectioned at a 7 um thickness, and then fixed with 4% parafolmaldehyde and
permeabilized with 1% Triton X-100.
Fixation 4 % parafolmadehyde at room temperature for 20 minutes.
Permeabilization 1% Triton X-100 at room temperature for 5 minutes.
Blocking Blocking buffer (PBS with BSA and goat serum) for 1 hour.
Primary Antibody Probing Primary antibody was added to the solution of blocking buffer (PBS with BSA and goat serum),
at 4˚C overnight.
Sample Wash The sample was washed with PBS with BSA and goat serum 5 times, each for 5 minutes.
Secondary Antibody Probing Secondary antibody was added to the solution of blocking buffer (PBS with BSA and goat serum)
with a 1:100 dilution ratio, for 1 hour.
Sample Wash The sample was washed with PBS with BSA and goat serum 7 times, each for 5 minutes.
Visualization Nikon Axiovert microscope.

4. What was the protocol?
No. Antigen
Primary
antibody
Primary
antibody
dilution ratio
Secondary
antibody
dilution ratio
1
Decellularised
cornea
STJ93888
Laminin a-1
antibody
1:50 1:00

5. What were the results?

6. What did the customer think?
Antibody Specificity:
Antibody RPPA Rating:
Testing results were provided independently by F. Abidin at Newcastle University. They
stated “Visible laminin staining was observed from this tissue section.”

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