3. Suzuki, Y. & Gojobori, T. The origin and evolution of Ebola and Marburg viruses. Mol Biol Evol 14, 800-806 (1997)
Marburg and Ebola diverged
nearly 10,000 years ago
Filoviruses have ancient origins
11. Deep sequencing to reveal new insights on EVD
Over 99% coverage of genome at
2000x
12. Origin and transmission of 2014 EVD outbreak
West African Ebola virus diverged from Central African lineages c. 2004
Diversity data consistent with single importation events from the reservoir
Ebola crosses from Guinea to Sierra Leone - 2 distinct clades enter via 12 travelers
13. Sierra Leone falls into three main genetic clusters
NS
S
NC
GUINEA
SIERRA LEONE CLUSTER 1
SIERRA LEONE CLUSTER 2
SIERRA LEONE CLUSTER 3
NP VP35 VP40 GP VP30 VP24 L POLYMERASE
19. An early and robust IgG response is indicative of survival
Baize, S. et al. Defective humoral responses and extensive intravascular apoptosis are associated with fatal outcome in Ebola virus-infected patients. Nature medicine 5, 423-426(1999)
23. Elevation of CRE and BUN is significant both to EVD and
outcome
Significance to EVD Significance to outcome
Dehydration and worsening renal function play significant role in
disease progression
24. Extensive liver damage from Ebola is often fatal
Significance to EVD Significance to outcome
ALT and AST significant to EVD; AST significant to outcome