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Methods for identification of plant diseases
Check for the symptoms:
Variation in symptoms in diseased plants may sometimes
lead to improper
Diagnosis..
Look for signs of disease
causal agents:
and spore-producing
bodies.
•Signs may include the
mycelia of a fungal
agent, fungal spores,
Identify plant part Affected: The symptoms
of some diseases are most commonly seen on
specific plant parts.
Check distribution of symptoms:
how the diseased plants are
distributed over the affected area
Check for host
specificity: Is the
problem occurring in
only one plant species or
are different plant
species .
Different techniques In Molecular Diagnosis
Polymerase Chain
Reaction (PCR)
PCR is used in the
diagnosis of plant
diseases has become
very common in
laboratory practice.
Efficiency for detection
of bacteria, fungi and
viruses is based on the
primer specificity.
LAMP A set of four specifically designed
primers.
Recognize six distinct sequences of the
target.
The amplification products.
Random Amplified
Polymorphic DNA (RAPD)
using short
usually 10
nucleotide
primers.
Polymorphi
sm of
amplified
fragments.
substitutio
ns or
deletions in
the priming
sites.
Restriction Fragment Length Polymorphism
(RFLP)
•Is a difference in homologous DNA
sequence.
•detected by the presence of fragments of
different lengths.
•after digestion of the DNA samples in
question with specific restriction
endonucleases.
Microarray
Technology
composed of
thousand of
specific probes
spotted onto a
solid surface.
Each probe is
complementary
to a specific DNA
sequence.
a signal that can
be detected and
analyzed.

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Plant disease management at molecular level.pptx

  • 1. Methods for identification of plant diseases Check for the symptoms: Variation in symptoms in diseased plants may sometimes lead to improper Diagnosis.. Look for signs of disease causal agents: and spore-producing bodies. •Signs may include the mycelia of a fungal agent, fungal spores, Identify plant part Affected: The symptoms of some diseases are most commonly seen on specific plant parts. Check distribution of symptoms: how the diseased plants are distributed over the affected area Check for host specificity: Is the problem occurring in only one plant species or are different plant species .
  • 2. Different techniques In Molecular Diagnosis Polymerase Chain Reaction (PCR) PCR is used in the diagnosis of plant diseases has become very common in laboratory practice. Efficiency for detection of bacteria, fungi and viruses is based on the primer specificity. LAMP A set of four specifically designed primers. Recognize six distinct sequences of the target. The amplification products. Random Amplified Polymorphic DNA (RAPD) using short usually 10 nucleotide primers. Polymorphi sm of amplified fragments. substitutio ns or deletions in the priming sites.
  • 3. Restriction Fragment Length Polymorphism (RFLP) •Is a difference in homologous DNA sequence. •detected by the presence of fragments of different lengths. •after digestion of the DNA samples in question with specific restriction endonucleases. Microarray Technology composed of thousand of specific probes spotted onto a solid surface. Each probe is complementary to a specific DNA sequence. a signal that can be detected and analyzed.