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Stem Cell quick guide 2013
- 1. Mesenchymal stem cells
Driving your MSC research
Human samples Mouse samples
Phenotyping of expanded MSCs
Human
• MSC Phenotyping Kit
• Fluorochrome-conjugated antibodies
against, e.g., CD73, CD90, CD105,
CD45, CD34, CD14, CD20, etc.
Mouse
• Fluorochrome-conjugated antibodies
against, e.g., Lineage markers, CD105,
CD117, Anti-Sca-1, CD45, CD116, etc.
• MACSQuant® Flow Cytometers
Molecular analysis of MSCs
• mRNA Isolation Kit and cDNA Synthesis Kit (for RT-PCR)
• Protein Isolation Kits (for ChIP or normal IPs)
• Microarray services
• SuperAmp™ Technology
• Bioinformatics
• MultiMACS™ M96 Separator
• Mitochondria isolation
Differentiation of MSCs
Human
• NH Differentiation Media
• Cytokines, e.g., TGF-β
• NH CFU-F
Mouse
• Cytokines, e.g., FGF-8b
Characterization of MSCs
Sample type
Frozen
• Dead Cell Removal Kit
• Pre-Separation Filters
Tissues
• gentleMACS™ Dissociator
• Pre-Separation Filters
Phenotyping of MSCs prior to expansion
Fluorochrome-conjugated
antibodies against, e.g.,
• CD271
• Anti-MSCA-1
• CD146
• CD105
• CD45
Phenotyping of MSCs prior to expansion
Fluorochrome-conjugated
antibodies against, e.g.,
• CD45
• Anti-Ter-119
• Anti-Sca-1
• CD105
Isolation of MSCs
Magnetic isolation
• CD271 (LNGFR) MicroBead Kits
• Anti-MSCA-1 (W8B2)
MicroBead Kit
• CD90 MicroBeads
• CD105 MicroBeads
• CD146 MicroBead Kit
• CD73 Antibodies
• Lineage Cell Depletion Kit
Flow sorting
• Fluorochrome-conjugated
antibodies
(markers as listed)
Plastic adherence
• NH Expansion Media
• CytoMix – MSC
Expansion of MSCs
• NH Expansion
Media
• CytoMix – MSC
• Cytokines, e.g.,
FGF-2, PDGF-BB,
TGF-β
Isolation of MSCs
Magnetic isolation
• Lineage Cell Depletion Kit
• CD105 MultiSort Kit (PE), CD117
MicroBeads, Anti-Sca-1
MicroBead Kit (FITC)
• CD45 MicroBeads, Anti-Ter-119
MicroBeads removal pre-culture
• CD11b MicroBeads removal
post-culture
Flow sorting
• Fluorochrome-
conjugated antibodies
(markers as listed)
Plastic adherence
Expansion of MSCs
• Cytokines, e.g., TGF-β, EGF
Translate research to clinical applications
• GMP Cytokines
• CliniMACS® System
• CliniMACS Reagents, e.g., CD271
Sample preparation Cell separation Cell analysis Cell culture Molecular applications Clinical applications
Quick
guide
inside!
MACS® Stem Cell Research
macs-stemcells.com
Germany/Austria/
Switzerland
Miltenyi Biotec GmbH
Friedrich-Ebert-Straße 68
51429 Bergisch Gladbach
Germany
Phone +49 2204 8306-0
Fax +49 2204 85197
macs@miltenyibiotec.de
USA/Canada
Miltenyi Biotec Inc.
2303 Lindbergh Street
Auburn, CA 95602, USA
Phone 800 FOR MACS
Phone +1 530 888 8871
Fax +1 530 888 8925
macs@miltenyibiotec.com
www.miltenyibiotec.com
Australia
Miltenyi Biotec
Australia Pty. Ltd.
Unit 16A, 2 Eden Park Drive
North Ryde, NSW 2113,
Australia
Phone +61 2 8877 7400
Fax +61 2 9889 5044
macs@miltenyibiotec.com.au
Benelux
Miltenyi Biotec B.V.
Schipholweg 68 H
2316 XE Leiden
The Netherlands
macs@miltenyibiotec.nl
Customer service:
The Netherlands
Phone 0800 4020120
Fax 0800 4020100
Belgium
Phone 0800 94016
Fax 0800 99626
Luxembourg
Phone 800 24971
Fax 800 24984
China
Miltenyi Biotec GmbH
Shanghai Office
Rm. 2309
No. 319 Xianxia Rd.
Shanghai 200051, P.R. China
Phone +86 21 62351005
Fax +86 21 62350953
macs@miltenyibiotec.com.cn
France
Miltenyi Biotec SAS
10 rue Mercoeur
75011 Paris, France
Phone +33 1 56 98 16 16
Fax +33 1 56 98 16 17
macs@miltenyibiotec.fr
Italy
Miltenyi Biotec S.r.l.
Via Persicetana, 2/D
40012 Calderara di Reno (BO)
Italy
Phone +39 051 6 460 411
Fax +39 051 6 460 499
macs@miltenyibiotec.it
Japan
Miltenyi Biotec K.K.
Nittsu-Eitai Building 5F
16-10 Fuyuki, Koto-ku,
Tokyo 135-0041, Japan
Phone +81 3 5646 8910
Fax +81 3 5646 8911
macs@miltenyibiotec.jp
Nordics and Baltics
Miltenyi Biotec Norden AB
Scheelevägen 17
223 70 Lund
Sweden
macs@miltenyibiotec.se
Customer service:
Sweden
Phone 0200-111 800
Fax 046-280 72 99
Denmark
Phone 80 20 30 10
Fax +46 46 280 72 99
Norway, Finland, Iceland,
and Baltic countries
Phone +46 46 280 72 80
Fax +46 46 280 72 99
Singapore
Miltenyi Biotec Asia Pacific Pte Ltd.
100 Beach Road
#28-06 to 28-08 Shaw Tower
Singapore 189702
Phone +65 6238 8183
Fax +65 6238 0302
macs@miltenyibiotec.com.sg
Spain
Miltenyi Biotec S.L.
C/Luis Buñuel 2
Ciudad de la Imagen
28223 Pozuelo de Alarcón (Madrid)
Spain
Phone +34 91 512 12 90
Fax +34 91 512 12 91
macs@miltenyibiotec.es
United Kingdom
Miltenyi Biotec Ltd.
Almac House, Church Lane
Bisley, Surrey GU24 9DR, UK
Phone +44 1483 799 800
Fax +44 1483 799 811
macs@miltenyibiotec.co.uk
130-097-901.02
Miltenyi Biotec provides products and services worldwide. Visit www.miltenyibiotec.com/local to find your nearest Miltenyi Biotec contact.
Unless otherwise specifically indicated, Miltenyi Biotec products and services are for research use only and not for therapeutic or diagnostic use.
MACS, MACSelect, MACSQuant, CliniMACS, gentleMACS, MultiMACS, and SuperAmp are either registered trademarks or trademarks of Miltenyi Biotec GmbH.
CryoStem, Pluriton, StainAlive, and Stemfect are all trademarks of Stemgent, Inc. Copyright © 2013 Miltenyi Biotec GmbH. All rights reserved.
- 2. Mesenchymal stem cells
Driving your MSC research
Human samples Mouse samples
Phenotyping of expanded MSCs
Human
• MSC Phenotyping Kit
• Fluorochrome-conjugated antibodies
against, e.g., CD73, CD90, CD105,
CD45, CD34, CD14, CD20, etc.
Mouse
• Fluorochrome-conjugated antibodies
against, e.g., Lineage markers, CD105,
CD117, Anti-Sca-1, CD45, CD116, etc.
• MACSQuant® Flow Cytometers
Molecular analysis of MSCs
• mRNA Isolation Kit and cDNA Synthesis Kit (for RT-PCR)
• Protein Isolation Kits (for ChIP or normal IPs)
• Microarray services
• SuperAmp™ Technology
• Bioinformatics
• MultiMACS™ M96 Separator
• Mitochondria isolation
Differentiation of MSCs
Human
• NH Differentiation Media
• Cytokines, e.g., TGF-β
• NH CFU-F
Mouse
• Cytokines, e.g., FGF-8b
Characterization of MSCs
Sample type
Frozen
• Dead Cell Removal Kit
• Pre-Separation Filters
Tissues
• gentleMACS™ Dissociator
• Pre-Separation Filters
Phenotyping of MSCs prior to expansion
Fluorochrome-conjugated
antibodies against, e.g.,
• CD271
• Anti-MSCA-1
• CD146
• CD105
• CD45
Phenotyping of MSCs prior to expansion
Fluorochrome-conjugated
antibodies against, e.g.,
• CD45
• Anti-Ter-119
• Anti-Sca-1
• CD105
Isolation of MSCs
Magnetic isolation
• CD271 (LNGFR) MicroBead Kits
• Anti-MSCA-1 (W8B2)
MicroBead Kit
• CD90 MicroBeads
• CD105 MicroBeads
• CD146 MicroBead Kit
• CD73 Antibodies
• Lineage Cell Depletion Kit
Flow sorting
• Fluorochrome-conjugated
antibodies
(markers as listed)
Plastic adherence
• NH Expansion Media
• CytoMix – MSC
Expansion of MSCs
• NH Expansion
Media
• CytoMix – MSC
• Cytokines, e.g.,
FGF-2, PDGF-BB,
TGF-β
Isolation of MSCs
Magnetic isolation
• Lineage Cell Depletion Kit
• CD105 MultiSort Kit (PE), CD117
MicroBeads, Anti-Sca-1
MicroBead Kit (FITC)
• CD45 MicroBeads, Anti-Ter-119
MicroBeads removal pre-culture
• CD11b MicroBeads removal
post-culture
Flow sorting
• Fluorochrome-
conjugated antibodies
(markers as listed)
Plastic adherence
Expansion of MSCs
• Cytokines, e.g., TGF-β, EGF
Translate research to clinical applications
• GMP Cytokines
• CliniMACS® System
• CliniMACS Reagents, e.g., CD271
Sample preparation Cell separation Cell analysis Cell culture Molecular applications Clinical applications
Quick
guide
inside!
MACS® Stem Cell Research
macs-stemcells.com
Germany/Austria/
Switzerland
Miltenyi Biotec GmbH
Friedrich-Ebert-Straße 68
51429 Bergisch Gladbach
Germany
Phone +49 2204 8306-0
Fax +49 2204 85197
macs@miltenyibiotec.de
USA/Canada
Miltenyi Biotec Inc.
2303 Lindbergh Street
Auburn, CA 95602, USA
Phone 800 FOR MACS
Phone +1 530 888 8871
Fax +1 530 888 8925
macs@miltenyibiotec.com
www.miltenyibiotec.com
Australia
Miltenyi Biotec
Australia Pty. Ltd.
Unit 16A, 2 Eden Park Drive
North Ryde, NSW 2113,
Australia
Phone +61 2 8877 7400
Fax +61 2 9889 5044
macs@miltenyibiotec.com.au
Benelux
Miltenyi Biotec B.V.
Schipholweg 68 H
2316 XE Leiden
The Netherlands
macs@miltenyibiotec.nl
Customer service:
The Netherlands
Phone 0800 4020120
Fax 0800 4020100
Belgium
Phone 0800 94016
Fax 0800 99626
Luxembourg
Phone 800 24971
Fax 800 24984
China
Miltenyi Biotec GmbH
Shanghai Office
Rm. 2309
No. 319 Xianxia Rd.
Shanghai 200051, P.R. China
Phone +86 21 62351005
Fax +86 21 62350953
macs@miltenyibiotec.com.cn
France
Miltenyi Biotec SAS
10 rue Mercoeur
75011 Paris, France
Phone +33 1 56 98 16 16
Fax +33 1 56 98 16 17
macs@miltenyibiotec.fr
Italy
Miltenyi Biotec S.r.l.
Via Persicetana, 2/D
40012 Calderara di Reno (BO)
Italy
Phone +39 051 6 460 411
Fax +39 051 6 460 499
macs@miltenyibiotec.it
Japan
Miltenyi Biotec K.K.
Nittsu-Eitai Building 5F
16-10 Fuyuki, Koto-ku,
Tokyo 135-0041, Japan
Phone +81 3 5646 8910
Fax +81 3 5646 8911
macs@miltenyibiotec.jp
Nordics and Baltics
Miltenyi Biotec Norden AB
Scheelevägen 17
223 70 Lund
Sweden
macs@miltenyibiotec.se
Customer service:
Sweden
Phone 0200-111 800
Fax 046-280 72 99
Denmark
Phone 80 20 30 10
Fax +46 46 280 72 99
Norway, Finland, Iceland,
and Baltic countries
Phone +46 46 280 72 80
Fax +46 46 280 72 99
Singapore
Miltenyi Biotec Asia Pacific Pte Ltd.
100 Beach Road
#28-06 to 28-08 Shaw Tower
Singapore 189702
Phone +65 6238 8183
Fax +65 6238 0302
macs@miltenyibiotec.com.sg
Spain
Miltenyi Biotec S.L.
C/Luis Buñuel 2
Ciudad de la Imagen
28223 Pozuelo de Alarcón (Madrid)
Spain
Phone +34 91 512 12 90
Fax +34 91 512 12 91
macs@miltenyibiotec.es
United Kingdom
Miltenyi Biotec Ltd.
Almac House, Church Lane
Bisley, Surrey GU24 9DR, UK
Phone +44 1483 799 800
Fax +44 1483 799 811
macs@miltenyibiotec.co.uk
130-097-901.02
Miltenyi Biotec provides products and services worldwide. Visit www.miltenyibiotec.com/local to find your nearest Miltenyi Biotec contact.
Unless otherwise specifically indicated, Miltenyi Biotec products and services are for research use only and not for therapeutic or diagnostic use.
MACS, MACSelect, MACSQuant, CliniMACS, gentleMACS, MultiMACS, and SuperAmp are either registered trademarks or trademarks of Miltenyi Biotec GmbH.
CryoStem, Pluriton, StainAlive, and Stemfect are all trademarks of Stemgent, Inc. Copyright © 2013 Miltenyi Biotec GmbH. All rights reserved.
- 3. Sample preparation Cell separation Cell analysis Cell culture Molecular applications Clinical applications Sample preparation Cell separation Cell analysis Cell culture Molecular applications Reprogramming
Embryonic and induced pluripotent stem cells
Pioneering solutions for ES and iPS cells research
Hematopoietic stem cells
Making headway in your HSC research
iPS cells
Do you differentiate your ES or iPS cells?
Characterization of reprogrammed cells
Human
• Extracellular PSC staining: TRA-1-60,
TRA-1-81, SSEA-3, SSEA-4, CD326,
CD324, etc.
• Extracellular differentiated cell staining:
SSEA-1
• Transcription factor staining: Oct4, Sox2,
Nanog, etc.
• MACSQuant® Flow Cytometers
Mouse
• Extracellular PSC staining: SSEA-1
• Extracellular differentiated cell staining:
Anti-Feeder antibodies
• Transcription factor staining: Oct4, Sox2,
Nanog, etc.
• MACSQuant Flow Cytometers
Isolation of reprogrammed cells
Magnetic isolation
Human
• Anti-TRA-1-60 MicroBead Kit
• Pluripotent Stem Cell MicroBeads
• Anti-SSEA-4 MicroBeads
• Isolation of TRA-1-81+
cells via indirect
magnetic labeling
• hES Cloning and Recovery Supplement
Mouse
• Anti-SSEA-1 MicroBeads
Flow sorting
• Fluorochrome-conjugated antibodies
(markers above)
Colony Picking
• StainAlive™ Antibodies
Generation of iPS cells
Human
• mRNA Reprogramming Reagents
• Pluriton™ Reprogramming Media
• Small molecules
Mouse
• Reprogramming Ecotropic Retrovirus
• Small Molecules
Differentiation of ES or iPS cells
Human or mouse
• Cytokines
• Small molecules
Note: Different cytokines are used in accordance
with the differentiation pathway.
Purification of ES or iPS cells prior to differentiation
Human
• Anti-TRA-1-60 MicroBead Kit
• Pluripotent Stem Cell MicroBeads
Mouse
• Pluripotent Stem Cell Isolation Kit
Differentiation of ES or iPS cells via
embryoid bodies
• Embryoid Body Dissociation Kit
• gentleMACS™ Dissociator
Monitoring differentiation by flow cytometry
or immunohistochemistry
• Antibodies, for example, use CD34 antibodies to analyze
cells during differentiation into hematopoietic or
endothelial lineages.
• MACSQuant Flow Cytometers
Purification of cells during or after differentiation
MicroBead reagents, for example, cell separation reagents
for differentiated cells, e.g.,
• Neural Crest Stem Cell MicroBeads
• CD34 MicroBeads
• CD144 (VE-Cadherin) MicroBeads
• CD309 (Flk-1) MicroBead Kit
• Anti-PSA-NCAM MicroBead Kit
• Anti-A2B5 MicroBead Kit
Human samples Mouse samples
Sample type
Frozen
• Dead Cell Removal Kit
• Pre-Separation Filters
PBMNCs or lysed
whole blood
• Red Blood Cell Lysis Solution
Isolation of HSCs
Magnetic isolation
• Lineage Cell Depletion Kit
+ CD117 MicroBeads
+ Anti-Sca-1 MicroBead Kit
(FITC)
• CD105 Multisort Kit (PE) +
Anti-Sca-1 MicroBead Kit
(FITC)
Flow sorting
Fluorochrome-conjugated
antibodies against, e.g.,
• Lineage markers
• Anti-Sca-1
• CD117 (c-kit)
• CD135 (Flt3R)Isolation of HSCs
Magnetic isolation
• CD34 MicroBead Kit
• CD133 MicroBead Kit
• Lineage Cell Depletion Kit
• CD117 MicroBead Kit
Flow sorting
Fluorochrome-conjugated
antibodies against, e.g.,
• CD34
• CD133
• CD45
• CD90
• CD38
Expansion of HSCs
• Cytokines, e.g., TPO, SCF, Flt3
Expansion of HSCs
• Cytokines, e.g., SCF, Flt3
Phenotyping of HSCs
Human
Fluorochrome-conjugated
antibodies against, e.g.,
• CD34
• CD133
• CD45
• CD90
• CD38
• MACSQuant®
Flow Cytometers
Mouse
Fluorochrome-conjugated
antibodies against, e.g.,
• Lineage markers
• Sca-1
• CD117 (c-kit)
• CD135 (Flt3R)
• MACSQuant
Flow Cytometers
Molecular analysis of HSCs
• mRNA Isolation Kit and
cDNA Synthesis Kit (for
RT-PCR)
• Protein Isolation Kits
(for ChIP or normal IPs)
• Microarray services
• SuperAmp™ Technology
• Bioinformatics
• MultiMACS™ M96 Separator
• Mitochondria isolation
CFU assays for HSCs
Human
• HSC-CFU Media
Differentiation of HSCs
Human or mouse
• Cytokines
Note: Different cytokines are
used in accordance
with the differentiation
pathway.
Characterization of HSCs
ES cells
Transfection of ES or iPS cells
Human
• Stemfect™ hESC DNA Transfection Kit
• Stemfect Enhanced hESC DNA Transfection Kit
• Stemfect RNA Transfection Kit
• MACSelect™ Products
Mouse
• Stemfect 2.0-mESC DNA Transfection Reagent
• MACSelect™ Products
Single-cell passaging of ES or iPS cells
Human
• hES Cloning and Recovery Supplement
• Anti-TRA-1-60 MicroBead Kit
• Pluripotent Stem Cell MicroBeads
Monitoring of pluripotency by flow cytometry or immunohistochemistry
Human
• Extracellular PSC staining: TRA-1-60, TRA-1-81, SSEA-3, SSEA-4, CD326, CD324, etc.
• Extracellular differentiated cell staining: SSEA-1
• Transcription factor staining: Oct4, Sox2, Nanog, etc.
• MACSQuant® Flow Cytometers
Mouse
• Extracellular PSC staining: SSEA-1
• Extracellular differentiated cell staining: Anti-Feeder antibodies
• Transcription factor staining: Oct4, Sox2, Nanog, etc.
• MACSQuant® Flow Cytometers
Translate research to clinical applications
• GMP Cytokines
• CliniMACS System
• CliniMACS® Reagents, e.g., CD34, CD133
Removal of feeder cells
Depleting
feeder cells
Human
• Anti-Fibroblast
MicroBeads
Mouse
• Feeder Removal
MicroBeads
Isolating pluripotent
stem cells
Human
• Anti-TRA-1-60
MicroBead Kit
• Pluripotent Stem
Cell MicroBeads
Mouse
• Anti-SSEA-1
MicroBeads
Culture of ES or iPS cells
Feeder-based
Human
• C ytokines, e.g., FGF2
• CryoStem™ Freezing Medium
• Small molecules
Mouse
• Cytokines, e.g., LIF
• Small molecules
Feeder-free
Human
• NutriStem™ Medium
• Cytokines, e.g., FGF2
• Small molecules
• CryoStem Freezing Medium
Mouse
• Cytokines, e.g., LIF
• Small molecules
- 4. Sample preparation Cell separation Cell analysis Cell culture Molecular applications Clinical applications Sample preparation Cell separation Cell analysis Cell culture Molecular applications Reprogramming
Embryonic and induced pluripotent stem cells
Pioneering solutions for ES and iPS cells research
Hematopoietic stem cells
Making headway in your HSC research
iPS cells
Do you differentiate your ES or iPS cells?
Characterization of reprogrammed cells
Human
• Extracellular PSC staining: TRA-1-60,
TRA-1-81, SSEA-3, SSEA-4, CD326,
CD324, etc.
• Extracellular differentiated cell staining:
SSEA-1
• Transcription factor staining: Oct4, Sox2,
Nanog, etc.
• MACSQuant® Flow Cytometers
Mouse
• Extracellular PSC staining: SSEA-1
• Extracellular differentiated cell staining:
Anti-Feeder antibodies
• Transcription factor staining: Oct4, Sox2,
Nanog, etc.
• MACSQuant Flow Cytometers
Isolation of reprogrammed cells
Magnetic isolation
Human
• Anti-TRA-1-60 MicroBead Kit
• Pluripotent Stem Cell MicroBeads
• Anti-SSEA-4 MicroBeads
• Isolation of TRA-1-81+
cells via indirect
magnetic labeling
• hES Cloning and Recovery Supplement
Mouse
• Anti-SSEA-1 MicroBeads
Flow sorting
• Fluorochrome-conjugated antibodies
(markers above)
Colony Picking
• StainAlive™ Antibodies
Generation of iPS cells
Human
• mRNA Reprogramming Reagents
• Pluriton™ Reprogramming Media
• Small molecules
Mouse
• Reprogramming Ecotropic Retrovirus
• Small Molecules
Differentiation of ES or iPS cells
Human or mouse
• Cytokines
• Small molecules
Note: Different cytokines are used in accordance
with the differentiation pathway.
Purification of ES or iPS cells prior to differentiation
Human
• Anti-TRA-1-60 MicroBead Kit
• Pluripotent Stem Cell MicroBeads
Mouse
• Pluripotent Stem Cell Isolation Kit
Differentiation of ES or iPS cells via
embryoid bodies
• Embryoid Body Dissociation Kit
• gentleMACS™ Dissociator
Monitoring differentiation by flow cytometry
or immunohistochemistry
• Antibodies, for example, use CD34 antibodies to analyze
cells during differentiation into hematopoietic or
endothelial lineages.
• MACSQuant Flow Cytometers
Purification of cells during or after differentiation
MicroBead reagents, for example, cell separation reagents
for differentiated cells, e.g.,
• Neural Crest Stem Cell MicroBeads
• CD34 MicroBeads
• CD144 (VE-Cadherin) MicroBeads
• CD309 (Flk-1) MicroBead Kit
• Anti-PSA-NCAM MicroBead Kit
• Anti-A2B5 MicroBead Kit
Human samples Mouse samples
Sample type
Frozen
• Dead Cell Removal Kit
• Pre-Separation Filters
PBMNCs or lysed
whole blood
• Red Blood Cell Lysis Solution
Isolation of HSCs
Magnetic isolation
• Lineage Cell Depletion Kit
+ CD117 MicroBeads
+ Anti-Sca-1 MicroBead Kit
(FITC)
• CD105 Multisort Kit (PE) +
Anti-Sca-1 MicroBead Kit
(FITC)
Flow sorting
Fluorochrome-conjugated
antibodies against, e.g.,
• Lineage markers
• Anti-Sca-1
• CD117 (c-kit)
• CD135 (Flt3R)Isolation of HSCs
Magnetic isolation
• CD34 MicroBead Kit
• CD133 MicroBead Kit
• Lineage Cell Depletion Kit
• CD117 MicroBead Kit
Flow sorting
Fluorochrome-conjugated
antibodies against, e.g.,
• CD34
• CD133
• CD45
• CD90
• CD38
Expansion of HSCs
• Cytokines, e.g., TPO, SCF, Flt3
Expansion of HSCs
• Cytokines, e.g., SCF, Flt3
Phenotyping of HSCs
Human
Fluorochrome-conjugated
antibodies against, e.g.,
• CD34
• CD133
• CD45
• CD90
• CD38
• MACSQuant®
Flow Cytometers
Mouse
Fluorochrome-conjugated
antibodies against, e.g.,
• Lineage markers
• Sca-1
• CD117 (c-kit)
• CD135 (Flt3R)
• MACSQuant
Flow Cytometers
Molecular analysis of HSCs
• mRNA Isolation Kit and
cDNA Synthesis Kit (for
RT-PCR)
• Protein Isolation Kits
(for ChIP or normal IPs)
• Microarray services
• SuperAmp™ Technology
• Bioinformatics
• MultiMACS™ M96 Separator
• Mitochondria isolation
CFU assays for HSCs
Human
• HSC-CFU Media
Differentiation of HSCs
Human or mouse
• Cytokines
Note: Different cytokines are
used in accordance
with the differentiation
pathway.
Characterization of HSCs
ES cells
Transfection of ES or iPS cells
Human
• Stemfect™ hESC DNA Transfection Kit
• Stemfect Enhanced hESC DNA Transfection Kit
• Stemfect RNA Transfection Kit
• MACSelect™ Products
Mouse
• Stemfect 2.0-mESC DNA Transfection Reagent
• MACSelect™ Products
Single-cell passaging of ES or iPS cells
Human
• hES Cloning and Recovery Supplement
• Anti-TRA-1-60 MicroBead Kit
• Pluripotent Stem Cell MicroBeads
Monitoring of pluripotency by flow cytometry or immunohistochemistry
Human
• Extracellular PSC staining: TRA-1-60, TRA-1-81, SSEA-3, SSEA-4, CD326, CD324, etc.
• Extracellular differentiated cell staining: SSEA-1
• Transcription factor staining: Oct4, Sox2, Nanog, etc.
• MACSQuant® Flow Cytometers
Mouse
• Extracellular PSC staining: SSEA-1
• Extracellular differentiated cell staining: Anti-Feeder antibodies
• Transcription factor staining: Oct4, Sox2, Nanog, etc.
• MACSQuant® Flow Cytometers
Translate research to clinical applications
• GMP Cytokines
• CliniMACS System
• CliniMACS® Reagents, e.g., CD34, CD133
Removal of feeder cells
Depleting
feeder cells
Human
• Anti-Fibroblast
MicroBeads
Mouse
• Feeder Removal
MicroBeads
Isolating pluripotent
stem cells
Human
• Anti-TRA-1-60
MicroBead Kit
• Pluripotent Stem
Cell MicroBeads
Mouse
• Anti-SSEA-1
MicroBeads
Culture of ES or iPS cells
Feeder-based
Human
• C ytokines, e.g., FGF2
• CryoStem™ Freezing Medium
• Small molecules
Mouse
• Cytokines, e.g., LIF
• Small molecules
Feeder-free
Human
• NutriStem™ Medium
• Cytokines, e.g., FGF2
• Small molecules
• CryoStem Freezing Medium
Mouse
• Cytokines, e.g., LIF
• Small molecules
- 5. Mesenchymal stem cells
Driving your MSC research
Human samples Mouse samples
Phenotyping of expanded MSCs
Human
• MSC Phenotyping Kit
• Fluorochrome-conjugated antibodies
against, e.g., CD73, CD90, CD105,
CD45, CD34, CD14, CD20, etc.
Mouse
• Fluorochrome-conjugated antibodies
against, e.g., Lineage markers, CD105,
CD117, Anti-Sca-1, CD45, CD116, etc.
• MACSQuant® Flow Cytometers
Molecular analysis of MSCs
• mRNA Isolation Kit and cDNA Synthesis Kit (for RT-PCR)
• Protein Isolation Kits (for ChIP or normal IPs)
• Microarray services
• SuperAmp™ Technology
• Bioinformatics
• MultiMACS™ M96 Separator
• Mitochondria isolation
Differentiation of MSCs
Human
• NH Differentiation Media
• Cytokines, e.g., TGF-β
• NH CFU-F
Mouse
• Cytokines, e.g., FGF-8b
Characterization of MSCs
Sample type
Frozen
• Dead Cell Removal Kit
• Pre-Separation Filters
Tissues
• gentleMACS™ Dissociator
• Pre-Separation Filters
Phenotyping of MSCs prior to expansion
Fluorochrome-conjugated
antibodies against, e.g.,
• CD271
• Anti-MSCA-1
• CD146
• CD105
• CD45
Phenotyping of MSCs prior to expansion
Fluorochrome-conjugated
antibodies against, e.g.,
• CD45
• Anti-Ter-119
• Anti-Sca-1
• CD105
Isolation of MSCs
Magnetic isolation
• CD271 (LNGFR) MicroBead Kits
• Anti-MSCA-1 (W8B2)
MicroBead Kit
• CD90 MicroBeads
• CD105 MicroBeads
• CD146 MicroBead Kit
• CD73 Antibodies
• Lineage Cell Depletion Kit
Flow sorting
• Fluorochrome-conjugated
antibodies
(markers as listed)
Plastic adherence
• NH Expansion Media
• CytoMix – MSC
Expansion of MSCs
• NH Expansion
Media
• CytoMix – MSC
• Cytokines, e.g.,
FGF-2, PDGF-BB,
TGF-β
Isolation of MSCs
Magnetic isolation
• Lineage Cell Depletion Kit
• CD105 MultiSort Kit (PE), CD117
MicroBeads, Anti-Sca-1
MicroBead Kit (FITC)
• CD45 MicroBeads, Anti-Ter-119
MicroBeads removal pre-culture
• CD11b MicroBeads removal
post-culture
Flow sorting
• Fluorochrome-
conjugated antibodies
(markers as listed)
Plastic adherence
Expansion of MSCs
• Cytokines, e.g., TGF-β, EGF
Translate research to clinical applications
• GMP Cytokines
• CliniMACS® System
• CliniMACS Reagents, e.g., CD271
Sample preparation Cell separation Cell analysis Cell culture Molecular applications Clinical applications
Quick
guide
inside!
MACS® Stem Cell Research
macs-stemcells.com
Germany/Austria/
Switzerland
Miltenyi Biotec GmbH
Friedrich-Ebert-Straße 68
51429 Bergisch Gladbach
Germany
Phone +49 2204 8306-0
Fax +49 2204 85197
macs@miltenyibiotec.de
USA/Canada
Miltenyi Biotec Inc.
2303 Lindbergh Street
Auburn, CA 95602, USA
Phone 800 FOR MACS
Phone +1 530 888 8871
Fax +1 530 888 8925
macs@miltenyibiotec.com
www.miltenyibiotec.com
Australia
Miltenyi Biotec
Australia Pty. Ltd.
Unit 16A, 2 Eden Park Drive
North Ryde, NSW 2113,
Australia
Phone +61 2 8877 7400
Fax +61 2 9889 5044
macs@miltenyibiotec.com.au
Benelux
Miltenyi Biotec B.V.
Schipholweg 68 H
2316 XE Leiden
The Netherlands
macs@miltenyibiotec.nl
Customer service:
The Netherlands
Phone 0800 4020120
Fax 0800 4020100
Belgium
Phone 0800 94016
Fax 0800 99626
Luxembourg
Phone 800 24971
Fax 800 24984
China
Miltenyi Biotec GmbH
Shanghai Office
Rm. 2309
No. 319 Xianxia Rd.
Shanghai 200051, P.R. China
Phone +86 21 62351005
Fax +86 21 62350953
macs@miltenyibiotec.com.cn
France
Miltenyi Biotec SAS
10 rue Mercoeur
75011 Paris, France
Phone +33 1 56 98 16 16
Fax +33 1 56 98 16 17
macs@miltenyibiotec.fr
Italy
Miltenyi Biotec S.r.l.
Via Persicetana, 2/D
40012 Calderara di Reno (BO)
Italy
Phone +39 051 6 460 411
Fax +39 051 6 460 499
macs@miltenyibiotec.it
Japan
Miltenyi Biotec K.K.
Nittsu-Eitai Building 5F
16-10 Fuyuki, Koto-ku,
Tokyo 135-0041, Japan
Phone +81 3 5646 8910
Fax +81 3 5646 8911
macs@miltenyibiotec.jp
Nordics and Baltics
Miltenyi Biotec Norden AB
Scheelevägen 17
223 70 Lund
Sweden
macs@miltenyibiotec.se
Customer service:
Sweden
Phone 0200-111 800
Fax 046-280 72 99
Denmark
Phone 80 20 30 10
Fax +46 46 280 72 99
Norway, Finland, Iceland,
and Baltic countries
Phone +46 46 280 72 80
Fax +46 46 280 72 99
Singapore
Miltenyi Biotec Asia Pacific Pte Ltd.
100 Beach Road
#28-06 to 28-08 Shaw Tower
Singapore 189702
Phone +65 6238 8183
Fax +65 6238 0302
macs@miltenyibiotec.com.sg
Spain
Miltenyi Biotec S.L.
C/Luis Buñuel 2
Ciudad de la Imagen
28223 Pozuelo de Alarcón (Madrid)
Spain
Phone +34 91 512 12 90
Fax +34 91 512 12 91
macs@miltenyibiotec.es
United Kingdom
Miltenyi Biotec Ltd.
Almac House, Church Lane
Bisley, Surrey GU24 9DR, UK
Phone +44 1483 799 800
Fax +44 1483 799 811
macs@miltenyibiotec.co.uk
130-097-901.02
Miltenyi Biotec provides products and services worldwide. Visit www.miltenyibiotec.com/local to find your nearest Miltenyi Biotec contact.
Unless otherwise specifically indicated, Miltenyi Biotec products and services are for research use only and not for therapeutic or diagnostic use.
MACS, MACSelect, MACSQuant, CliniMACS, gentleMACS, MultiMACS, and SuperAmp are either registered trademarks or trademarks of Miltenyi Biotec GmbH.
CryoStem, Pluriton, StainAlive, and Stemfect are all trademarks of Stemgent, Inc. Copyright © 2013 Miltenyi Biotec GmbH. All rights reserved.