1. A preliminary assessment of phytochemical constituents and antimicrobial properties in three common traditional medicinal plants of Madurai by ANANDARAJ P (06BOT01) ANANTHVINOTHAN I (06BOT02) ARUN PANDI N (06BOT04) Under the guidance of Dr. G. C. ABRAHAM

2. INTRODUCTION Resurgence of interest on herbal medicine and traditional practices. FRLHT, MSSRF – agency involved in protecting the indigenous knowledge and information.

3. AIM AND OBJECTIVES To collect information on common plants that are used by the community as medicine. To study the specific uses of the selected plants. To conduct phytochemical tests and an antimicrobial assay on the selected plants.

4. METHODOLOGY Field survey and collection of data. (a) Interviewing the practitioners and public with prepared questionnaire (b) Documentation of information in the form of tables Phytochemical investigation of shoot extracts. (c) Solvent extraction 3. Antimicrobial assay

5. QUESTIONNAIRE (model)

6. A mini-survey on the therapeutic uses of certain common medicinal herbs at Madurai and its suburbs

7. Binomial – Vitex negundo Linn Common – Cat leaf chaste tree Family – Verbenaceae Tamil - Notchi Binomial – Coccinia indica W&A Common – Ivy-gourd Family – Cucurbitaceae Tamil – Kovai kodi Binomial – Cardiospermum halicacabum Linn Common – Ballon vine, winter cherry. Family – Sapindaceae Tamil - Mudakkathan

8. PLANT EXTRACTION PROCEDURE Dry coarse shoot material extracted by Soxhlet method and in iodine flask Extracted with distilled water Extracted with alcohol Alcoholic extract Aqueous extract Greenish black residue Dark green residue

9. PHYTOCHEMICALTESTS

10. Phytochemical screening of selected plant species (End results) PE -Petroleum Ether, OH –Methanol and Aq stands for Aqueous extracts.

11. ANTIMICROBIAL ASSAY Selection of microbial culture. Preparation of Muller-Hinton agar plates. Spreading the microbial culture. Wells creation over the medium. Loading the wells with extracts. Analyzing the zone of inhibition after incubation.

12. Test for the antimicrobial activity of three selected species of medicinal plants RESPONSES: - indicate absence of any effect. +, ++ & +++ indicate the extent of the zone of inhibition in increasing order.

13. ANTIMICROBIAL ASSAY WITH SELECTED STRAINS Control (Staphyllococcus aureus) Control (E.coli) Methanol extract of Coccinia showing inhibition activity over E.coli.. Coccinia indica W&A pet ether extract showing resistance against S.aureus growth.

14. DISCUSSION AND CONCLUSION Many common plants have medicinal property. Of these, the three selected plants have some special constituents. Among the selected strains, S.aureus (G+) is controlled more than E.coli (G-). Of the three plants, Coccinia is more effective than the other two. Among the extracts, organic is more effective than aqueous and in that methanol is better than per ether extract.

15. THANK YOU