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Hermi Indita/Endang Retnowati PEMERIKSAAN LIMFOSIT T CD4 + DENGAN FLOW CYTOMETRY

Aplikasi Klinis Flow Cytometry ,[object Object],[object Object],[object Object],[object Object]

Flow Cytometer BD FACSCalibur™ System

[object Object],[object Object],Excitation Optics (laser) Fluidics–Hydrodynamic Focusing Sistem Fluida

Sistem Optik ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

[object Object],[object Object],[object Object],Sistem komputer

Prosedur Pemeriksaan Limfosit CD4 + Dengan Menggunakan Flow Sitometer BD FACSCalibur™ System ,[object Object]

Interpretasi Hasil ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

[object Object],Contoh printout pemeriksaan limfosit T CD4 + menggunakan Flow Cytometer BD FACSCalibur

Kalibrasi ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Current CD4 monitoring tests and technologies include ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

T – cell subsets T T T C H CD3 CD3 CD3 CD8 CD4

Foreign Invader Specific Antibody Antigen

Analyze Two-Color Direct Staining Incubate

[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Right Angle Light Detector  Cell Complexity Forward Light Detector  Cell Surface Area Incident Light Source Properties of FSC and SSC

Side Scatter Forward Light Scatter Lysed Whole Blood 0 200 400 600 800 1000 0 200 400 600 800 1000 Lymphocytes Monocytes Neutrophils largest and most complex population smallest and least complex population

Limfosit subset CD3 T Llimfosit CD4 Helper T limfosit CD8 Supresor T limfosit CD19 B limfosit CD45 Lekosit CD3/CD4 Helper / Inducer T limfosit CD3/CD8 Supresor / sitotoksik limfosit CD16/CD56 NK sel CD4:CD8 Helper / supresor ratio

Menghitung Σ absolut CD4 ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Aplikasi klinis flow sitometer ,[object Object],[object Object],[object Object],[object Object],[object Object]

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