An Add On Nitrifying MBR As Effluent Polishing Technique For Ee2

ISEST 2009, Shanghai, June 4, 2009 Bart De Gusseme B. Pycke, T. Hennebel, A. Marcoen, S. E. Vlaeminck, H. Noppe, N. Boon, W. Verstraete Laboratory of Microbial Ecology & Technology, Ghent University, Belgium ,[object Object],Bart De Gusseme An add-on MBR as effluent polishing technique for 17α-ethinylestradiol ISEST 2009, Shanghai, June 4, 2009

Introduction ,[object Object],[object Object],[object Object],Bart De Gusseme An add-on MBR as effluent polishing technique for 17α-ethinylestradiol ISEST 2009, Shanghai, June 4, 2009

17α-ethinylestradiol ,[object Object],[object Object],[object Object],Bart De Gusseme An add-on MBR as effluent polishing technique for 17α-ethinylestradiol ISEST 2009, Shanghai, June 4, 2009

Need for effluent post-treatment ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Bart De Gusseme An add-on MBR as effluent polishing technique for 17α-ethinylestradiol ISEST 2009, Shanghai, June 4, 2009

Need for effluent post-treatment ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Bart De Gusseme An add-on MBR as effluent polishing technique for 17α-ethinylestradiol ISEST 2009, Shanghai, June 4, 2009 Need for alternative and sustainable WWTP effluent polishing techniques

Nitrifying cultures and EE2 removal ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Bart De Gusseme An add-on MBR as effluent polishing technique for 17α-ethinylestradiol ISEST 2009, Shanghai, June 4, 2009

Research questions ,[object Object],[object Object],[object Object],Commercially available NEC (ABIL) (Grommen et al., 2002; Aquacult, 211: 115-124; LabMET) AOB: Nitrosomonas NOB: Nitrobacter & Nitrospira Heterotrophic strains Bart De Gusseme An add-on MBR as effluent polishing technique for 17α-ethinylestradiol ISEST 2009, Shanghai, June 4, 2009

1. Batch incubation experiments ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Bart De Gusseme An add-on MBR as effluent polishing technique for 17α-ethinylestradiol ISEST 2009, Shanghai, June 4, 2009

1. Batch incubation experiments Heat-treated NEC B iomass-free control Bart De Gusseme An add-on MBR as effluent polishing technique for 17α-ethinylestradiol ISEST 2009, Shanghai, June 4, 2009 NEC in WWTP effluent 9 μg EE2/g VSS.h NEC in minimal medium 9 μg EE2/g VSS.h NH 4 + -N : 52.5  < 2 mg/L

[object Object],1. Batch incubation experiments Bart De Gusseme An add-on MBR as effluent polishing technique for 17α-ethinylestradiol ISEST 2009, Shanghai, June 4, 2009 ,[object Object],[object Object],[object Object]

2. Mechanism ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Bart De Gusseme An add-on MBR as effluent polishing technique for 17α-ethinylestradiol ISEST 2009, Shanghai, June 4, 2009

2. Mechanism Bart De Gusseme An add-on MBR as effluent polishing technique for 17α-ethinylestradiol ISEST 2009, Shanghai, June 4, 2009 EE2 removal ---- Nitrate production 96  1 % removal without ATU Complete nitrification 38  1 % removal with ATU No nitrification ,[object Object]

3. Continuous EE2 removal in MBR A. Reactor design Bart De Gusseme An add-on MBR as effluent polishing technique for 17α-ethinylestradiol ISEST 2009, Shanghai, June 4, 2009 Influent (tap water containing EE2) Plate membranes Effluent Gas diffuser Sprue with vacuum Air

3. Continuous EE2 removal in MBR B. Operational parameters ,[object Object],[object Object],[object Object],[object Object],Bart De Gusseme An add-on MBR as effluent polishing technique for 17α-ethinylestradiol ISEST 2009, Shanghai, June 4, 2009 100 μg/L 83 ng/L

3. Continuous EE2 removal in MBR C. Reactor performance Bart De Gusseme An add-on MBR as effluent polishing technique for 17α-ethinylestradiol ISEST 2009, Shanghai, June 4, 2009 ,[object Object],[object Object],[object Object]

Future perspectives Bart De Gusseme An add-on MBR as effluent polishing technique for 17α-ethinylestradiol ISEST 2009, Shanghai, June 4, 2009 ,[object Object],[object Object],Discharge to surface waters Add-on MBR with NEC Wastewater Treatment Plant Effluent Activated sludge If necessary, d osage of NH 4 + -N to 1 mg/L

Conclusions ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Bart De Gusseme An add-on MBR as effluent polishing technique for 17α-ethinylestradiol ISEST 2009, Shanghai, June 4, 2009

Acknowledgements Bart De Gusseme (Aspirant), Tom Hennebel (project no. 7741-02) and Siegfried E. Vlaeminck (Aspirant) are supported by the Fund of Scientific Research (FWO)-Flanders Benny Pycke is supported by a PhD grant (IWT-SB/53360) of the Institute for the Promotion of Innovation through Science and Technology in Flanders (IWT) This study was part of the EU Neptune project (ContractNo. 036845, SUSTDEV-2005-3.II.3.2), which was financed by the EU Commission and the Sixth Framework (FP6-2005-Global-4). Bart De Gusseme An add-on MBR as effluent polishing technique for 17α-ethinylestradiol ISEST 2009, Shanghai, June 4, 2009

B. De Gusseme, B. Pycke, T. Hennebel, A. Marcoen, S. E. Vlaeminck, H. Noppe, N. Boon & W. Verstraete ,[object Object],[email_address] LabMET.UGent.be Bart De Gusseme An add-on MBR as effluent polishing technique for 17α-ethinylestradiol ISEST 2009, Shanghai, June 4, 2009

An Add On Nitrifying MBR As Effluent Polishing Technique For Ee2

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