SlideShare una empresa de Scribd logo
1 de 25
 
What is Chromatography? Chromatography is a technique for separating mixtures into their components in order to analyze, identify, purify, and/or quantify the mixture or components. Separate ,[object Object],[object Object],[object Object],[object Object],Components Mixture
Uses for Chromatography ,[object Object],[object Object],[object Object],[object Object],[object Object]
Uses for Chromatography ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Definition of Chromatography ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Definition of Chromatography ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Illustration of Chromatography Mixture Components Separation Stationary Phase Mobile Phase Components Affinity to Stationary Phase Affinity to Mobile Phase Blue ---------------- Insoluble in Mobile Phase Black                     Red                    Yellow                          
 
Types of Chromatography ,[object Object],[object Object],[object Object],[object Object],Types of Chromatography
(A) uses charge, (B) uses pores, and (C) uses covalent bonds to create the differential affinities among the mixture components for the stationary phase.
Principles of Paper Chromatography ,[object Object],[object Object],[object Object]
Paper Chromatography Experiment What Color is that Sharpie?
Overview of the Experiment ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Materials List
Preparing the Isopropanol Solutions ,[object Object],[object Object]
Preparing the Chromatography Strips ,[object Object],[object Object],[object Object],[object Object]
Developing the Chromatograms ,[object Object],[object Object],[object Object],[object Object],[object Object]
Developing the Chromatograms
Developing the Chromatograms
 
Observing the Chromatograms Concentration of Isopropanol 0% 20% 50% 70% 100%
Black Dye ,[object Object],[object Object],[object Object],Concentration of Isopropanol 0% 20% 50% 70% 100%
Blue Dye Concentration of Isopropanol 0% 20% 50% 70% 100% 1. Dye separated – blue 2. Not very soluble in low concentrations of isopropanol 3. Completely soluble in high concentrations of isopropanol
Green Dye Concentration of Isopropanol 0% 20% 50% 70% 100% 1. Dye separated – blue and yellow 2. Blue – Soluble in concentrations of isopropanol >20% 3. Yellow – Soluble in concentrations of isopropanol >0%
Red Dye ,[object Object],[object Object],[object Object],Concentration of Isopropanol 0% 20% 50% 70% 100% 3. Red –  slightly soluble in low concentrations of isopropanol, and more soluble in concentrations of isopropanol >20%

Más contenido relacionado

La actualidad más candente

Paper Chromatography PPT (new)
Paper Chromatography PPT (new)Paper Chromatography PPT (new)
Paper Chromatography PPT (new)
shaisejacob
 
Paper chromatography(final)
Paper chromatography(final)Paper chromatography(final)
Paper chromatography(final)
Myquie de Guzman
 

La actualidad más candente (20)

Paper chromatography
Paper chromatographyPaper chromatography
Paper chromatography
 
Chromatography
ChromatographyChromatography
Chromatography
 
Column chromatography
Column chromatographyColumn chromatography
Column chromatography
 
Paper Chromatography PPT (new)
Paper Chromatography PPT (new)Paper Chromatography PPT (new)
Paper Chromatography PPT (new)
 
PAPER CHROMATOGRAPHY
PAPER CHROMATOGRAPHYPAPER CHROMATOGRAPHY
PAPER CHROMATOGRAPHY
 
Gel permeation chromatography copy
Gel permeation chromatography   copyGel permeation chromatography   copy
Gel permeation chromatography copy
 
Column Chromatography.pptx
Column Chromatography.pptxColumn Chromatography.pptx
Column Chromatography.pptx
 
Paper chromatography
Paper chromatographyPaper chromatography
Paper chromatography
 
Thin layer chromatography by khaleel S.G
Thin layer chromatography by khaleel S.GThin layer chromatography by khaleel S.G
Thin layer chromatography by khaleel S.G
 
Paper chromatography
Paper chromatographyPaper chromatography
Paper chromatography
 
Hplc
Hplc Hplc
Hplc
 
Paper chromatography(final)
Paper chromatography(final)Paper chromatography(final)
Paper chromatography(final)
 
Chromatography
ChromatographyChromatography
Chromatography
 
CHROMATOGRAPHY
CHROMATOGRAPHY CHROMATOGRAPHY
CHROMATOGRAPHY
 
Paper Chromatography
Paper Chromatography Paper Chromatography
Paper Chromatography
 
CHROMATOGRAPHY
CHROMATOGRAPHYCHROMATOGRAPHY
CHROMATOGRAPHY
 
Chromatography
ChromatographyChromatography
Chromatography
 
Classification of chromatography
Classification of chromatographyClassification of chromatography
Classification of chromatography
 
Paper chromatography
Paper chromatographyPaper chromatography
Paper chromatography
 
Theory of chromatographic separations
Theory of chromatographic separationsTheory of chromatographic separations
Theory of chromatographic separations
 

Destacado

Chromatography and its types
Chromatography and its typesChromatography and its types
Chromatography and its types
nadeem akhter
 
Statistical mechanics
Statistical mechanics Statistical mechanics
Statistical mechanics
Kumar
 
Types of chromatography
Types of chromatographyTypes of chromatography
Types of chromatography
Fizan Chee
 
Presentation on principle of paper chromatography and Rf Value
Presentation on principle of paper chromatography and Rf ValuePresentation on principle of paper chromatography and Rf Value
Presentation on principle of paper chromatography and Rf Value
Manoj Kumar Tekuri
 
Thin layer chromatography
Thin layer chromatographyThin layer chromatography
Thin layer chromatography
Guruprasad Rao
 
TLC, thin layer chromatography
TLC, thin layer chromatographyTLC, thin layer chromatography
TLC, thin layer chromatography
shaisejacob
 

Destacado (20)

Paper chromatography experiment
Paper chromatography experimentPaper chromatography experiment
Paper chromatography experiment
 
Chromatography and its types
Chromatography and its typesChromatography and its types
Chromatography and its types
 
Statistical Mechanics & Thermodynamics 2: Physical Kinetics
Statistical Mechanics & Thermodynamics 2: Physical KineticsStatistical Mechanics & Thermodynamics 2: Physical Kinetics
Statistical Mechanics & Thermodynamics 2: Physical Kinetics
 
Statistical mechanics
Statistical mechanics Statistical mechanics
Statistical mechanics
 
Types of chromatography
Types of chromatographyTypes of chromatography
Types of chromatography
 
Attracting Curiosity - EAInnovates2015
Attracting Curiosity - EAInnovates2015Attracting Curiosity - EAInnovates2015
Attracting Curiosity - EAInnovates2015
 
Magnets
MagnetsMagnets
Magnets
 
Magnets in orthodontics.1
Magnets in orthodontics.1Magnets in orthodontics.1
Magnets in orthodontics.1
 
Provoking Original Ideas from Student Makers
Provoking Original Ideas from Student Makers Provoking Original Ideas from Student Makers
Provoking Original Ideas from Student Makers
 
Presentation on principle of paper chromatography and Rf Value
Presentation on principle of paper chromatography and Rf ValuePresentation on principle of paper chromatography and Rf Value
Presentation on principle of paper chromatography and Rf Value
 
Planar Chromatography
Planar ChromatographyPlanar Chromatography
Planar Chromatography
 
Rflp technology
Rflp technologyRflp technology
Rflp technology
 
Magnets
MagnetsMagnets
Magnets
 
chromatographic techniques
chromatographic techniqueschromatographic techniques
chromatographic techniques
 
Types of chromatographic methods
Types of chromatographic methodsTypes of chromatographic methods
Types of chromatographic methods
 
Thin Layer Chroatography
Thin Layer ChroatographyThin Layer Chroatography
Thin Layer Chroatography
 
Thin layer chromatography
Thin layer chromatographyThin layer chromatography
Thin layer chromatography
 
Thin layer chromatography (tlc)
Thin layer chromatography (tlc)Thin layer chromatography (tlc)
Thin layer chromatography (tlc)
 
TLC, thin layer chromatography
TLC, thin layer chromatographyTLC, thin layer chromatography
TLC, thin layer chromatography
 
Thin layer chromatography
Thin layer chromatographyThin layer chromatography
Thin layer chromatography
 

Similar a Paper Chromatography

Chemistry review c1 c5
Chemistry review c1 c5Chemistry review c1 c5
Chemistry review c1 c5
Mary Rodriguez
 
Paper chromatography
Paper chromatographyPaper chromatography
Paper chromatography
Akshay Pareek
 
Chromatography_Md. Asaduzzaman_SEU
Chromatography_Md. Asaduzzaman_SEUChromatography_Md. Asaduzzaman_SEU
Chromatography_Md. Asaduzzaman_SEU
ShOuro DeXter
 

Similar a Paper Chromatography (20)

Paper chromatography
Paper chromatographyPaper chromatography
Paper chromatography
 
Paper-Chromatography.ppt
Paper-Chromatography.pptPaper-Chromatography.ppt
Paper-Chromatography.ppt
 
Chemistry review c1 c5
Chemistry review c1 c5Chemistry review c1 c5
Chemistry review c1 c5
 
Chromatography
ChromatographyChromatography
Chromatography
 
chromatography .pptx
chromatography .pptxchromatography .pptx
chromatography .pptx
 
Paper Chromatography, principles, introduction and applications
Paper Chromatography, principles, introduction and applicationsPaper Chromatography, principles, introduction and applications
Paper Chromatography, principles, introduction and applications
 
Chromatography ankit
Chromatography ankitChromatography ankit
Chromatography ankit
 
Chromatography by narayan sarkar and simi baruah
Chromatography by  narayan sarkar and simi baruahChromatography by  narayan sarkar and simi baruah
Chromatography by narayan sarkar and simi baruah
 
Chromatography
ChromatographyChromatography
Chromatography
 
Chromatography p.c
Chromatography p.cChromatography p.c
Chromatography p.c
 
Basics of Chromatography.ppt
Basics of Chromatography.pptBasics of Chromatography.ppt
Basics of Chromatography.ppt
 
Chromatography by narayan sarkar and simi baruah new version
Chromatography by  narayan sarkar and simi baruah  new versionChromatography by  narayan sarkar and simi baruah  new version
Chromatography by narayan sarkar and simi baruah new version
 
Paper chromatography
Paper chromatographyPaper chromatography
Paper chromatography
 
Chromatography
ChromatographyChromatography
Chromatography
 
Chromatography.pptx
Chromatography.pptxChromatography.pptx
Chromatography.pptx
 
Chromatography.pdf
Chromatography.pdfChromatography.pdf
Chromatography.pdf
 
1.chromatography
1.chromatography1.chromatography
1.chromatography
 
Chromatography_Md. Asaduzzaman_SEU
Chromatography_Md. Asaduzzaman_SEUChromatography_Md. Asaduzzaman_SEU
Chromatography_Md. Asaduzzaman_SEU
 
Paper chromatography by ck
Paper chromatography by ckPaper chromatography by ck
Paper chromatography by ck
 
CHROMATOGRAPHIC TECHNIQUES.pptx
CHROMATOGRAPHIC TECHNIQUES.pptxCHROMATOGRAPHIC TECHNIQUES.pptx
CHROMATOGRAPHIC TECHNIQUES.pptx
 

Más de David Genis (20)

WCRage Handbook
WCRage HandbookWCRage Handbook
WCRage Handbook
 
Wc rage handbook
Wc rage handbookWc rage handbook
Wc rage handbook
 
Metric conversion house
Metric conversion houseMetric conversion house
Metric conversion house
 
The everyday metric system
The everyday metric systemThe everyday metric system
The everyday metric system
 
Experimental Design
Experimental DesignExperimental Design
Experimental Design
 
Class policies and procedures
Class policies and proceduresClass policies and procedures
Class policies and procedures
 
Class policies and procedures
Class policies and proceduresClass policies and procedures
Class policies and procedures
 
Class policies and procedures
Class policies and proceduresClass policies and procedures
Class policies and procedures
 
Class policies and procedures
Class policies and proceduresClass policies and procedures
Class policies and procedures
 
Work
WorkWork
Work
 
Electricity
ElectricityElectricity
Electricity
 
Energy forms and_changes
Energy forms and_changesEnergy forms and_changes
Energy forms and_changes
 
0708 types of_energy
0708 types of_energy0708 types of_energy
0708 types of_energy
 
Work
WorkWork
Work
 
Work
WorkWork
Work
 
Cub simple lesson01_presentation
Cub simple lesson01_presentationCub simple lesson01_presentation
Cub simple lesson01_presentation
 
Ttl7 09
Ttl7 09Ttl7 09
Ttl7 09
 
Lightning noaa
Lightning noaaLightning noaa
Lightning noaa
 
Presentation3
Presentation3Presentation3
Presentation3
 
Humidity
HumidityHumidity
Humidity
 

Último

Histor y of HAM Radio presentation slide
Histor y of HAM Radio presentation slideHistor y of HAM Radio presentation slide
Histor y of HAM Radio presentation slide
vu2urc
 
Artificial Intelligence: Facts and Myths
Artificial Intelligence: Facts and MythsArtificial Intelligence: Facts and Myths
Artificial Intelligence: Facts and Myths
Joaquim Jorge
 
+971581248768>> SAFE AND ORIGINAL ABORTION PILLS FOR SALE IN DUBAI AND ABUDHA...
+971581248768>> SAFE AND ORIGINAL ABORTION PILLS FOR SALE IN DUBAI AND ABUDHA...+971581248768>> SAFE AND ORIGINAL ABORTION PILLS FOR SALE IN DUBAI AND ABUDHA...
+971581248768>> SAFE AND ORIGINAL ABORTION PILLS FOR SALE IN DUBAI AND ABUDHA...
?#DUbAI#??##{{(☎️+971_581248768%)**%*]'#abortion pills for sale in dubai@
 

Último (20)

04-2024-HHUG-Sales-and-Marketing-Alignment.pptx
04-2024-HHUG-Sales-and-Marketing-Alignment.pptx04-2024-HHUG-Sales-and-Marketing-Alignment.pptx
04-2024-HHUG-Sales-and-Marketing-Alignment.pptx
 
Understanding Discord NSFW Servers A Guide for Responsible Users.pdf
Understanding Discord NSFW Servers A Guide for Responsible Users.pdfUnderstanding Discord NSFW Servers A Guide for Responsible Users.pdf
Understanding Discord NSFW Servers A Guide for Responsible Users.pdf
 
Histor y of HAM Radio presentation slide
Histor y of HAM Radio presentation slideHistor y of HAM Radio presentation slide
Histor y of HAM Radio presentation slide
 
ProductAnonymous-April2024-WinProductDiscovery-MelissaKlemke
ProductAnonymous-April2024-WinProductDiscovery-MelissaKlemkeProductAnonymous-April2024-WinProductDiscovery-MelissaKlemke
ProductAnonymous-April2024-WinProductDiscovery-MelissaKlemke
 
TrustArc Webinar - Stay Ahead of US State Data Privacy Law Developments
TrustArc Webinar - Stay Ahead of US State Data Privacy Law DevelopmentsTrustArc Webinar - Stay Ahead of US State Data Privacy Law Developments
TrustArc Webinar - Stay Ahead of US State Data Privacy Law Developments
 
Advantages of Hiring UIUX Design Service Providers for Your Business
Advantages of Hiring UIUX Design Service Providers for Your BusinessAdvantages of Hiring UIUX Design Service Providers for Your Business
Advantages of Hiring UIUX Design Service Providers for Your Business
 
Connector Corner: Accelerate revenue generation using UiPath API-centric busi...
Connector Corner: Accelerate revenue generation using UiPath API-centric busi...Connector Corner: Accelerate revenue generation using UiPath API-centric busi...
Connector Corner: Accelerate revenue generation using UiPath API-centric busi...
 
Axa Assurance Maroc - Insurer Innovation Award 2024
Axa Assurance Maroc - Insurer Innovation Award 2024Axa Assurance Maroc - Insurer Innovation Award 2024
Axa Assurance Maroc - Insurer Innovation Award 2024
 
Driving Behavioral Change for Information Management through Data-Driven Gree...
Driving Behavioral Change for Information Management through Data-Driven Gree...Driving Behavioral Change for Information Management through Data-Driven Gree...
Driving Behavioral Change for Information Management through Data-Driven Gree...
 
From Event to Action: Accelerate Your Decision Making with Real-Time Automation
From Event to Action: Accelerate Your Decision Making with Real-Time AutomationFrom Event to Action: Accelerate Your Decision Making with Real-Time Automation
From Event to Action: Accelerate Your Decision Making with Real-Time Automation
 
Artificial Intelligence: Facts and Myths
Artificial Intelligence: Facts and MythsArtificial Intelligence: Facts and Myths
Artificial Intelligence: Facts and Myths
 
GenCyber Cyber Security Day Presentation
GenCyber Cyber Security Day PresentationGenCyber Cyber Security Day Presentation
GenCyber Cyber Security Day Presentation
 
Developing An App To Navigate The Roads of Brazil
Developing An App To Navigate The Roads of BrazilDeveloping An App To Navigate The Roads of Brazil
Developing An App To Navigate The Roads of Brazil
 
Powerful Google developer tools for immediate impact! (2023-24 C)
Powerful Google developer tools for immediate impact! (2023-24 C)Powerful Google developer tools for immediate impact! (2023-24 C)
Powerful Google developer tools for immediate impact! (2023-24 C)
 
presentation ICT roal in 21st century education
presentation ICT roal in 21st century educationpresentation ICT roal in 21st century education
presentation ICT roal in 21st century education
 
Workshop - Best of Both Worlds_ Combine KG and Vector search for enhanced R...
Workshop - Best of Both Worlds_ Combine  KG and Vector search for  enhanced R...Workshop - Best of Both Worlds_ Combine  KG and Vector search for  enhanced R...
Workshop - Best of Both Worlds_ Combine KG and Vector search for enhanced R...
 
Strategize a Smooth Tenant-to-tenant Migration and Copilot Takeoff
Strategize a Smooth Tenant-to-tenant Migration and Copilot TakeoffStrategize a Smooth Tenant-to-tenant Migration and Copilot Takeoff
Strategize a Smooth Tenant-to-tenant Migration and Copilot Takeoff
 
The 7 Things I Know About Cyber Security After 25 Years | April 2024
The 7 Things I Know About Cyber Security After 25 Years | April 2024The 7 Things I Know About Cyber Security After 25 Years | April 2024
The 7 Things I Know About Cyber Security After 25 Years | April 2024
 
+971581248768>> SAFE AND ORIGINAL ABORTION PILLS FOR SALE IN DUBAI AND ABUDHA...
+971581248768>> SAFE AND ORIGINAL ABORTION PILLS FOR SALE IN DUBAI AND ABUDHA...+971581248768>> SAFE AND ORIGINAL ABORTION PILLS FOR SALE IN DUBAI AND ABUDHA...
+971581248768>> SAFE AND ORIGINAL ABORTION PILLS FOR SALE IN DUBAI AND ABUDHA...
 
How to Troubleshoot Apps for the Modern Connected Worker
How to Troubleshoot Apps for the Modern Connected WorkerHow to Troubleshoot Apps for the Modern Connected Worker
How to Troubleshoot Apps for the Modern Connected Worker
 

Paper Chromatography

  • 1.  
  • 2.
  • 3.
  • 4.
  • 5.
  • 6.
  • 7. Illustration of Chromatography Mixture Components Separation Stationary Phase Mobile Phase Components Affinity to Stationary Phase Affinity to Mobile Phase Blue ---------------- Insoluble in Mobile Phase Black         Red        Yellow          
  • 8.  
  • 9.
  • 10. (A) uses charge, (B) uses pores, and (C) uses covalent bonds to create the differential affinities among the mixture components for the stationary phase.
  • 11.
  • 12. Paper Chromatography Experiment What Color is that Sharpie?
  • 13.
  • 14.
  • 15.
  • 16.
  • 17.
  • 20.  
  • 21. Observing the Chromatograms Concentration of Isopropanol 0% 20% 50% 70% 100%
  • 22.
  • 23. Blue Dye Concentration of Isopropanol 0% 20% 50% 70% 100% 1. Dye separated – blue 2. Not very soluble in low concentrations of isopropanol 3. Completely soluble in high concentrations of isopropanol
  • 24. Green Dye Concentration of Isopropanol 0% 20% 50% 70% 100% 1. Dye separated – blue and yellow 2. Blue – Soluble in concentrations of isopropanol >20% 3. Yellow – Soluble in concentrations of isopropanol >0%
  • 25.