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Enzymes found at home
Biological washing powders
Enzyme power!
Qn : How would enzymes help to clean clothes with stains on them? Qn:  Can any enzyme be used in biological washing powders?
* Characteristics of enzymes
Some characteristics of enzymes learnt earlier…. ,[object Object],[object Object],[object Object],[object Object]
Effect of temperature on enzyme action Note :   For every increase in 10 °C in temperature, the rate of reaction doubles (until the optimum temp. is reached) (temp. at which enzyme is most active) denaturation
Why does enzyme activity increase as temperature increase? ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Why does enzyme activity decrease at higher temperature? ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
 
Denaturation ,[object Object],[object Object],[object Object]
Effects of pH on enzyme action (optimum pH) (enzyme activity ↓ )
Points to note on pH: ,[object Object],[object Object],[object Object]
Different enzymes = different  optimum pH
Reaction rate vs substrate concentration curve What do you observe?
Limiting factor ,[object Object],[object Object],[object Object]
[substrate]  ↑ ; rate of reaction ↑ till X Further ↑  ; no ↑  in rate [saturation of enzymes (limiting factor); amt. of products formed per unit time is the same] More enzyme more products formed per unit time till Y (enzyme conc. becomes the limiting factor)
Co-enzymes ,[object Object],[object Object],[object Object]
Applying study tip (mneumonics) ,[object Object],[object Object],[object Object],[object Object]
Summary (Using Mnemonics) ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],pecific (lock and key hypothesis) inute amounts (renewable) eversible reactions emperature o-enzymes may be needed lter rates of chemical reactions (lowers activation energy) H nzyme & substrate concentrations
Overview  Biological Molecules Water and Living Organisms  Carbohydrates, Fats and Proteins  Enzymes  Lock & Key hypothesis Mode of Action Factors affecting enzyme reactions Speed up chemical Rxn Not chemically altered at the end Of Rxn Lowering Activation Energy Specificity Temperature pH
Quiz!
Quiz… ,[object Object],Enzymes are affected by temperature. Regulating temperature ensures that the enzymes work within its temperature range to prevent denaturation.
Quiz ,[object Object],[object Object],[object Object],[object Object]

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  • 4. Qn : How would enzymes help to clean clothes with stains on them? Qn: Can any enzyme be used in biological washing powders?
  • 6.
  • 7. Effect of temperature on enzyme action Note : For every increase in 10 °C in temperature, the rate of reaction doubles (until the optimum temp. is reached) (temp. at which enzyme is most active) denaturation
  • 8.
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  • 10.  
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  • 12. Effects of pH on enzyme action (optimum pH) (enzyme activity ↓ )
  • 13.
  • 14. Different enzymes = different optimum pH
  • 15. Reaction rate vs substrate concentration curve What do you observe?
  • 16.
  • 17. [substrate] ↑ ; rate of reaction ↑ till X Further ↑ ; no ↑ in rate [saturation of enzymes (limiting factor); amt. of products formed per unit time is the same] More enzyme more products formed per unit time till Y (enzyme conc. becomes the limiting factor)
  • 18.
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  • 20.
  • 21. Overview Biological Molecules Water and Living Organisms Carbohydrates, Fats and Proteins Enzymes Lock & Key hypothesis Mode of Action Factors affecting enzyme reactions Speed up chemical Rxn Not chemically altered at the end Of Rxn Lowering Activation Energy Specificity Temperature pH
  • 22. Quiz!
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  • 24.