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Design and Analysis Strategies for DNA microarray data: hits to targets
Organization of the presentation ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Molecular based discover ,[object Object],[object Object],[object Object]
[object Object],[object Object]
Microarrays ,[object Object],[object Object],[object Object],[object Object],[object Object]
Applications of microarrays ,[object Object],[object Object],[object Object],[object Object],[object Object]
Microarray ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Affymetrix ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Preprocessing methods (BMC Bioinformatics  2006,  7 :105)
Preprocessing ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
common experimental inquires ,[object Object],[object Object],[object Object],[object Object],[object Object]
LIMMA : Linear Models for microarray analysis  ( Subramanian, Tamayo, et al.  ( 2005, PNAS 102, 15545-15550  ) ) ,[object Object],[object Object],[object Object],[object Object],[object Object]
Examples of comparisons
mock experimental design ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
steps involved ,[object Object],[object Object],[object Object],[object Object]
Interpretation of results ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Significance Analysis of Microarrays ,[object Object],[object Object],[object Object],[object Object]
Experimental designs supported by SAM (Chu, G., Narasimhan, B., Tibshirani, R. & Tusher, V. (2002), Signicance analysis of microarrays (sam) software)
Sample input format (Chu, G., Narasimhan, B., Tibshirani, R. & Tusher, V. (2002), Signicance analysis of microarrays (sam) software)
SAM statistics  (Chu, G., Narasimhan, B., Tibshirani, R. & Tusher, V. (2002), Signicance analysis of microarrays (sam) software)
 
SAM plot
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Gene set enrichment analysis (GSEA) ,[object Object],[object Object],[object Object],[object Object],[object Object]
Methodology ,[object Object],[object Object],[object Object],[object Object]
Subramanian, Tamayo, et al. ( 2005, PNAS 102, 15545-15550 )
Leading edge subset Subramanian, Tamayo, et al.  ( 2005, PNAS 102, 15545-15550 )
Subramanian, Tamayo, et al.  ( 2005, PNAS 102, 15545-15550 )
Novel methodology based on gene set enrichment ,[object Object],[object Object],[object Object]
Caveats ,[object Object],[object Object]
Enriched gene sets Phenotype  (http://www.broad.mit.edu/gsea/resources/gsea_pnas_results/p53_C2.Gsea/gsea_report_for_WT_1130958999391.html)
Enriched gene sets in mutant  (http://www.broad.mit.edu/gsea/resources/gsea_pnas_results/p53_C2.Gsea/gsea_report_for_MUT_1130958999391.html)
RNA interference ,[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object]
Endocytotic pathways ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Gene Ontology (Description) ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
 
biomaRt ( Bioconductor interface to BioMart Software Suit [ http://www.biomart.org/ ] )  (The biomaRt user’s guide Steffen Durinck, Wolfgang Huber)
 
 
GO based functional characterization of gene sets using topGO ,[object Object],[object Object],[object Object]
Alexa et al.  Bioinformatics ,  13, 1600-1607,  2006
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
A.Alexa et al.  Bioinformatics , 13, 1600-1607,  2006
 
 
 
 
 
 
Clone ID ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
 
Relational Database Development ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
 
References ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Acknowledgements ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

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