These slides are from 3DSIG 2014, presented on July 11.
I describe our investigation of internal symmetry in protein structures. This is quite common (24% of domains), and has many implications for function, folding, and evolution.
I introduce the CE-Symm method, described in
Myers-Turnbull, D., Bliven, S. E., Rose, P. W., Aziz, Z. K., Youkharibache, P., Bourne, P. E., & Prlić, A. (2014). Systematic Detection of Internal Symmetry in Proteins Using CE-Symm. Journal of Molecular Biology, 426(11), 2255–2268. doi:10.1016/j.jmb.2014.03.010
I discuss the results from running CE-Symm across the PDB, as well as some particularly compelling examples.
See also my poster by the same title for more details.
3DSIG 2014 Presentation: Systematic detection of internal symmetry in proteins
1. Spencer Bliven
July 11, 2014
3DSIG 2014
Myers-Turnbull, D., Bliven, S. E., Rose, P. W., Aziz, Z. K., Youkharibache, P., Bourne, P.
E., & Prlić, A. (2014). Systematic Detection of Internal Symmetry in Proteins Using CE-
Symm. Journal of Molecular Biology, 426(11), 2255–2268. PMID 24681267
6. ! Evolution
! Identify duplications & fusions
! Many examples of homologous quaternary symmetric/
internally symmetric proteins
! Tradeoff between monomer & oligomer
Lee and Blaber. PNAS (2011) vol. 108 (1) pp. 126-30
7. E. Coli DNA polymerase III beta
subunit [1mmi]
! 2 chains (C2 crystal axis)
Human proliferating cell nuclear
antigen [1VYM]
! 3 chains (C3 crystal axis)
8. E. Coli DNA polymerase III beta
subunit [1mmi]
! 2 chains
! 6 domains (pseudo C6)
Human proliferating cell nuclear
antigen [1VYM]
! 3 chains
! 6 domains (pseudo C6)
9. ! 2-3 chains
! 6 domains
! 12 structural repeats (pseudo D6)
Ancient 12-mer?
Ancient 6-mer
Bacterial DimerEukaryotic/Archaeal/
Viral Trimer
Kelman, Z., & O'Donnell, M. (1995). Nucleic Acids Research, 23(18), 3613–3620.
Neuwald, A. F., & Poleksic, A. (2000). Nucleic Acids Research, 28(18), 3570–3580.
10. ! Extends Combinatorial
Extension (CE) algorithm
for structural alignment
! Web server:
source.rcsb.org/
jfatcatserver/symmetry.jsp
! Download & Source code:
github.com/rcsb/symmetry
(LGPL)
Shindyalov, I. N., & Bourne, P. E. (1998). Protein Engineering,
11(9), 739–747.
Jia, Y., Dewey, T. G., Shindyalov, I. N., & Bourne, P. E. (2004).
J Comput Biol, 11(5), 787–799.
11. Fibroblast Growth
Factor [3JUT]
120° 120°
Myers-Turnbull, D., Bliven, S. E., Rose, P. W., Aziz, Z. K., Youkharibache, P., Bourne, P.
E., & Prlić, A. (2014). Journal of Molecular Biology, 426(11), 2255–2268.
12. Fibroblast Growth
Factor [3JUT]
120° 120°
Myers-Turnbull, D., Bliven, S. E., Rose, P. W., Aziz, Z. K., Youkharibache, P., Bourne, P.
E., & Prlić, A. (2014). Journal of Molecular Biology, 426(11), 2255–2268.
15. ! All domains from SCOPe 2.03
! Interactive results:
source.rcsb.org/jfatcatserver/scopResults.jsp
! Underestimate based on conservative thresholds
SCOP Class Superfamilies % Symmetric
α 507 18.5%
β 354 24.6%
α/β 244 16.8%
α+β 551 14.3%
Multi-domain 66 4.5%
Membrane 109 23.8%
Overall 1831 18.0%
16. ! PTS sorbitol transporter subunit IIA
! Novel fold
! Solved by the Protein Structure Initiative
! Structural alignment reveals a conserved sequence
motif between halves
[2F9H]
17. ! 18-24% of domains have internal symmetry
! Symmetry gives clues about duplication events
! Symmetry is deeply tied to protein function
! CE-Symm can accurately detect internal symmetry
d1su3a2 D1pt2a_ d1c5ka1 d1k3ia3 d1h9ya2
18. ! UC San Diego/RCSB
! Douglas Myers-Turnbull
! Andreas Prlić
! Peter Rose
! Zaid Aziz
! Milton Saier
! RCSB & Bourne Lab
members
! NIH
! Philip Bourne
! Philippe Youkharibache
! David Landsman
! Paul Scherrer Institute
! Guido Capitani & Lab
members
Resources:
! source.rcsb.org/jfatcatserver/
symmetry.jsp
! github.com/rcsb/symmetry
! Poster 25
! www.slideshare.net/sbliven
! Funding: NSF, NIH, DOE,
Open Science Grid
19.
20. Glyoxalase I from
Clostridium
acetobutylicum [3HDP]
(Nickel; Dimer)
Glyoxalase I from E.
coli [1F9Z]
(Nickel; Dimer)
1,2-dihydroxy-
naphthalene
dioxygenase from
Pseudomonas sp.
strain C18 [2EHZ]
(Iron; Octamer)
21. Glyoxalase I from
Clostridium
acetobutylicum [3HDP]
(Nickel; Dimer)
Glyoxalase I from E.
coli [1F9Z]
(Nickel; Dimer)
1,2-dihydroxy-
naphthalene
dioxygenase from
Pseudomonas sp.
strain C18 [2EHZ]
(Iron; Octamer)